Description of Streptococcus thalassemiae sp. nov., a Bacterium Isolated from Human Blood
Blood is a precious biological liquid that is normally sterile. Therefore, bacteria in the bloodstream are shown a priori anomaly. A blood culture is systematically performed to diagnose the cause of the bacteremia. Indeed, a patient received in our service had a thalassemia major and underwent a ge...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2023/3802590 |
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| author | Fatou Samba Diouf Mamadou Beye Mapenda Gaye Babacar Mbaye Stephane Alibar Mariema Sarr Gregory Dubourg Jean-Christophe Lagier Cheikh Sokhna Florence Fenollar Pierre-Edouard Fournier Cheikh Ibrahima Lo |
| author_facet | Fatou Samba Diouf Mamadou Beye Mapenda Gaye Babacar Mbaye Stephane Alibar Mariema Sarr Gregory Dubourg Jean-Christophe Lagier Cheikh Sokhna Florence Fenollar Pierre-Edouard Fournier Cheikh Ibrahima Lo |
| author_sort | Fatou Samba Diouf |
| collection | DOAJ |
| description | Blood is a precious biological liquid that is normally sterile. Therefore, bacteria in the bloodstream are shown a priori anomaly. A blood culture is systematically performed to diagnose the cause of the bacteremia. Indeed, a patient received in our service had a thalassemia major and underwent a genoidentical transplant. Then, a blood test was performed to diagnose a four-day fever. In this context, we have isolated strain Marseille-Q2617 from the blood sample. It revealed a new bacterial strain that belongs to the genus Streptococcus. It is a Gram-positive coccus, nonmotile, and nonspore forming. The major fatty acid found is hexadecanoic acid, with 49.5%. A taxonomic method was used to characterize the strain by studying their phenotypic, phylogenetic, and genomic characteristics. In addition, sequence analysis of the 16S rRNA gene shows that the strain Marseille-Q2617 has 99.94% sequence similarity to Streptococcus mitis. Average nucleotide identity (ANI) analysis for strain Marseille-Q2617T showed the highest similarity of 92.9% with S. mitis. The DNA-DNA hybridization value obtained (50.2%) between strain Marseille-Q2607 and S. mitis, its closest related species, was below the recommended threshold (<70%). Strain Marseille-Q2617T has a genome size of 2.02 Mbp with 40.5 mol% of G + C content. Based on these results, we propose a new species of the genus Streptococcus, for which the name Streptococcus thalassemiae sp. nov., Marseille-Q2617T (=CSUR Q2617 = CECT 30109) was proposed. |
| format | Article |
| id | doaj-art-f4fa6dc7a03e4b3c80ace8dd92bde2e4 |
| institution | Kabale University |
| issn | 1687-9198 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-f4fa6dc7a03e4b3c80ace8dd92bde2e42025-08-20T03:25:23ZengWileyInternational Journal of Microbiology1687-91982023-01-01202310.1155/2023/3802590Description of Streptococcus thalassemiae sp. nov., a Bacterium Isolated from Human BloodFatou Samba Diouf0Mamadou Beye1Mapenda Gaye2Babacar Mbaye3Stephane Alibar4Mariema Sarr5Gregory Dubourg6Jean-Christophe Lagier7Cheikh Sokhna8Florence Fenollar9Pierre-Edouard Fournier10Cheikh Ibrahima Lo11Aix Marseille UniversityIHU-Mediterranean InfectionAix Marseille UniversityAix Marseille UniversityAix Marseille UniversityAix Marseille UniversityAix Marseille UniversityAix Marseille UniversityAix Marseille UniversityIHU-Mediterranean InfectionIHU-Mediterranean InfectionIHU-Mediterranean InfectionBlood is a precious biological liquid that is normally sterile. Therefore, bacteria in the bloodstream are shown a priori anomaly. A blood culture is systematically performed to diagnose the cause of the bacteremia. Indeed, a patient received in our service had a thalassemia major and underwent a genoidentical transplant. Then, a blood test was performed to diagnose a four-day fever. In this context, we have isolated strain Marseille-Q2617 from the blood sample. It revealed a new bacterial strain that belongs to the genus Streptococcus. It is a Gram-positive coccus, nonmotile, and nonspore forming. The major fatty acid found is hexadecanoic acid, with 49.5%. A taxonomic method was used to characterize the strain by studying their phenotypic, phylogenetic, and genomic characteristics. In addition, sequence analysis of the 16S rRNA gene shows that the strain Marseille-Q2617 has 99.94% sequence similarity to Streptococcus mitis. Average nucleotide identity (ANI) analysis for strain Marseille-Q2617T showed the highest similarity of 92.9% with S. mitis. The DNA-DNA hybridization value obtained (50.2%) between strain Marseille-Q2607 and S. mitis, its closest related species, was below the recommended threshold (<70%). Strain Marseille-Q2617T has a genome size of 2.02 Mbp with 40.5 mol% of G + C content. Based on these results, we propose a new species of the genus Streptococcus, for which the name Streptococcus thalassemiae sp. nov., Marseille-Q2617T (=CSUR Q2617 = CECT 30109) was proposed.http://dx.doi.org/10.1155/2023/3802590 |
| spellingShingle | Fatou Samba Diouf Mamadou Beye Mapenda Gaye Babacar Mbaye Stephane Alibar Mariema Sarr Gregory Dubourg Jean-Christophe Lagier Cheikh Sokhna Florence Fenollar Pierre-Edouard Fournier Cheikh Ibrahima Lo Description of Streptococcus thalassemiae sp. nov., a Bacterium Isolated from Human Blood International Journal of Microbiology |
| title | Description of Streptococcus thalassemiae sp. nov., a Bacterium Isolated from Human Blood |
| title_full | Description of Streptococcus thalassemiae sp. nov., a Bacterium Isolated from Human Blood |
| title_fullStr | Description of Streptococcus thalassemiae sp. nov., a Bacterium Isolated from Human Blood |
| title_full_unstemmed | Description of Streptococcus thalassemiae sp. nov., a Bacterium Isolated from Human Blood |
| title_short | Description of Streptococcus thalassemiae sp. nov., a Bacterium Isolated from Human Blood |
| title_sort | description of streptococcus thalassemiae sp nov a bacterium isolated from human blood |
| url | http://dx.doi.org/10.1155/2023/3802590 |
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