Production of HMF-derivatives from wine residues using Saccharomyces cerevisiae as whole-cell biocatalyst
Abstract Background There is an urgent need to develop bioprocesses independent of fossil resources to address resource depletion and mitigate environmental harm. Transitioning to a bio-based economy requires prioritizing chemical production processes that utilize renewable resources, ensuring susta...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2025-01-01
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| Series: | Bioresources and Bioprocessing |
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| Online Access: | https://doi.org/10.1186/s40643-025-00840-5 |
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| author | Joana T. Cunha Aloia Romaní Lucília Domingues |
| author_facet | Joana T. Cunha Aloia Romaní Lucília Domingues |
| author_sort | Joana T. Cunha |
| collection | DOAJ |
| description | Abstract Background There is an urgent need to develop bioprocesses independent of fossil resources to address resource depletion and mitigate environmental harm. Transitioning to a bio-based economy requires prioritizing chemical production processes that utilize renewable resources, ensuring sustainability and environmental responsibility. 5-Hydroxymethylfurfural (HMF) and its derivatives are promising building blocks, ranked among the top 12 bio-based molecules derived from biomass. This study investigates the potential of wine residues as substrates for HMF production and explores the yeast Saccharomyces cerevisiae, a robust industrial microbial cell factory, as a whole-cell biocatalyst for converting HMF into high-value compounds, offering an alternative to chemical synthesis. Findings Several S. cerevisiae strains were compared for their ability to convert HMF, demonstrating varying capacities for oxidation or reduction. For the first time, HMF derivatives with potential industrial applications were produced using an HMF-rich hydrolysate obtained from sustainable processing of wine-growing waste, such as grape pomace and must surplus. The selected yeast strain was engineered to express the oxidoreductase enzyme of HMF/Furfural from Cupriavidua basilensis strain HMF14, resulting in a 15-fold increase in the accumulation of oxidized derivatives such as 2,5-furandicarboxylic acid (FDCA). Conclusions These findings highlight the potential of leveraging wine residues and engineered S. cerevisiae strains to develop sustainable bioprocesses for producing valuable HMF derivatives, thereby contributing to the advancement of bio-based chemical production. Graphical Abstract |
| format | Article |
| id | doaj-art-f4ee7bd1cc4549aaa0941ab1b39fdd00 |
| institution | Kabale University |
| issn | 2197-4365 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj-art-f4ee7bd1cc4549aaa0941ab1b39fdd002025-02-02T12:06:20ZengSpringerOpenBioresources and Bioprocessing2197-43652025-01-0112111010.1186/s40643-025-00840-5Production of HMF-derivatives from wine residues using Saccharomyces cerevisiae as whole-cell biocatalystJoana T. Cunha0Aloia Romaní1Lucília Domingues2CEB - Centre of Biological Engineering, University of MinhoDepartamento de Enxeñaría Química, Facultade de Ciencias, Universidade de VigoCEB - Centre of Biological Engineering, University of MinhoAbstract Background There is an urgent need to develop bioprocesses independent of fossil resources to address resource depletion and mitigate environmental harm. Transitioning to a bio-based economy requires prioritizing chemical production processes that utilize renewable resources, ensuring sustainability and environmental responsibility. 5-Hydroxymethylfurfural (HMF) and its derivatives are promising building blocks, ranked among the top 12 bio-based molecules derived from biomass. This study investigates the potential of wine residues as substrates for HMF production and explores the yeast Saccharomyces cerevisiae, a robust industrial microbial cell factory, as a whole-cell biocatalyst for converting HMF into high-value compounds, offering an alternative to chemical synthesis. Findings Several S. cerevisiae strains were compared for their ability to convert HMF, demonstrating varying capacities for oxidation or reduction. For the first time, HMF derivatives with potential industrial applications were produced using an HMF-rich hydrolysate obtained from sustainable processing of wine-growing waste, such as grape pomace and must surplus. The selected yeast strain was engineered to express the oxidoreductase enzyme of HMF/Furfural from Cupriavidua basilensis strain HMF14, resulting in a 15-fold increase in the accumulation of oxidized derivatives such as 2,5-furandicarboxylic acid (FDCA). Conclusions These findings highlight the potential of leveraging wine residues and engineered S. cerevisiae strains to develop sustainable bioprocesses for producing valuable HMF derivatives, thereby contributing to the advancement of bio-based chemical production. Graphical Abstracthttps://doi.org/10.1186/s40643-025-00840-5HMFFDCABiocatalysisSaccharomyces cerevisiaeMicrowave heating technologyWine byproducts |
| spellingShingle | Joana T. Cunha Aloia Romaní Lucília Domingues Production of HMF-derivatives from wine residues using Saccharomyces cerevisiae as whole-cell biocatalyst Bioresources and Bioprocessing HMF FDCA Biocatalysis Saccharomyces cerevisiae Microwave heating technology Wine byproducts |
| title | Production of HMF-derivatives from wine residues using Saccharomyces cerevisiae as whole-cell biocatalyst |
| title_full | Production of HMF-derivatives from wine residues using Saccharomyces cerevisiae as whole-cell biocatalyst |
| title_fullStr | Production of HMF-derivatives from wine residues using Saccharomyces cerevisiae as whole-cell biocatalyst |
| title_full_unstemmed | Production of HMF-derivatives from wine residues using Saccharomyces cerevisiae as whole-cell biocatalyst |
| title_short | Production of HMF-derivatives from wine residues using Saccharomyces cerevisiae as whole-cell biocatalyst |
| title_sort | production of hmf derivatives from wine residues using saccharomyces cerevisiae as whole cell biocatalyst |
| topic | HMF FDCA Biocatalysis Saccharomyces cerevisiae Microwave heating technology Wine byproducts |
| url | https://doi.org/10.1186/s40643-025-00840-5 |
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