Tumour-infiltrating lymphocyte therapy landscape: prospects and challenges

Tumour-infiltrating lymphocyte (TIL) therapy has emerged as a promising adoptive cell transfer strategy for solid tumours. The recent accelerated approval of lifileucel by the Food and Drug Administration marks a significant milestone in the clinical application of TIL therapy. This review comprehen...

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Main Authors: Arkadiusz Z Dudek, Jeffrey Johnson, Ruqin Chen, Ali Rezazadeh
Format: Article
Language:English
Published: BMJ Publishing Group 2025-08-01
Series:BMJ Oncology
Online Access:https://bmjoncology.bmj.com/content/4/1/e000566.full
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author Arkadiusz Z Dudek
Jeffrey Johnson
Ruqin Chen
Ali Rezazadeh
author_facet Arkadiusz Z Dudek
Jeffrey Johnson
Ruqin Chen
Ali Rezazadeh
author_sort Arkadiusz Z Dudek
collection DOAJ
description Tumour-infiltrating lymphocyte (TIL) therapy has emerged as a promising adoptive cell transfer strategy for solid tumours. The recent accelerated approval of lifileucel by the Food and Drug Administration marks a significant milestone in the clinical application of TIL therapy. This review comprehensively examines the historical development, biology, clinical efficacy, safety and limitations of TIL therapy. We explore advancements in TIL manufacturing, including novel culture techniques, genetic modifications and automation, to enhance scalability and effectiveness. Despite promising results, TIL therapy faces challenges such as high-dose interleukin-2 toxicity, complex manufacturing processes and immune evasion mechanisms. Emerging strategies, including checkpoint inhibitor combinations, engineered TIL constructs and metabolic reprogramming, aim to improve TIL therapeutic efficacy. This review provides insights into the evolving landscape of TIL therapy and its potential to enhance current cancer immunotherapy.
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issn 2752-7948
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publishDate 2025-08-01
publisher BMJ Publishing Group
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series BMJ Oncology
spelling doaj-art-f4e5b595332c47b79cff288b2eeddeea2025-08-20T03:38:39ZengBMJ Publishing GroupBMJ Oncology2752-79482025-08-014110.1136/bmjonc-2024-000566Tumour-infiltrating lymphocyte therapy landscape: prospects and challengesArkadiusz Z Dudek0Jeffrey Johnson1Ruqin Chen2Ali Rezazadeh3Aff2 grid.185648.60000000121750319University of Illinois Cancer Center Chicago IL USA1School of Pharmacy, University of Wisconsin, Madison, WI, USADepartment of Medicine, Mayo Clinic, Jacksonville, Florida, USAMedical Oncology, Mayo Clinic, Rochester, Minnesota, USATumour-infiltrating lymphocyte (TIL) therapy has emerged as a promising adoptive cell transfer strategy for solid tumours. The recent accelerated approval of lifileucel by the Food and Drug Administration marks a significant milestone in the clinical application of TIL therapy. This review comprehensively examines the historical development, biology, clinical efficacy, safety and limitations of TIL therapy. We explore advancements in TIL manufacturing, including novel culture techniques, genetic modifications and automation, to enhance scalability and effectiveness. Despite promising results, TIL therapy faces challenges such as high-dose interleukin-2 toxicity, complex manufacturing processes and immune evasion mechanisms. Emerging strategies, including checkpoint inhibitor combinations, engineered TIL constructs and metabolic reprogramming, aim to improve TIL therapeutic efficacy. This review provides insights into the evolving landscape of TIL therapy and its potential to enhance current cancer immunotherapy.https://bmjoncology.bmj.com/content/4/1/e000566.full
spellingShingle Arkadiusz Z Dudek
Jeffrey Johnson
Ruqin Chen
Ali Rezazadeh
Tumour-infiltrating lymphocyte therapy landscape: prospects and challenges
BMJ Oncology
title Tumour-infiltrating lymphocyte therapy landscape: prospects and challenges
title_full Tumour-infiltrating lymphocyte therapy landscape: prospects and challenges
title_fullStr Tumour-infiltrating lymphocyte therapy landscape: prospects and challenges
title_full_unstemmed Tumour-infiltrating lymphocyte therapy landscape: prospects and challenges
title_short Tumour-infiltrating lymphocyte therapy landscape: prospects and challenges
title_sort tumour infiltrating lymphocyte therapy landscape prospects and challenges
url https://bmjoncology.bmj.com/content/4/1/e000566.full
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