AG-NGS: A powerful and user-friendly computing application for the semi-automated preparation of next-generation sequencing libraries using open liquid handling platforms
Protocol Summary We have developed automatic genomics NGS (AG-NGS), a computing application that allows an open liquid handling platform to be transformed into a library preparation station without losing the potential of an open platform. Implementation of AG-NGS does not require programming experi...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2017-01-01
|
| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/000114504 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850152504667406336 |
|---|---|
| author | Sergio Callejas Rebeca Álvarez Alberto Benguria Ana Dopazo |
| author_facet | Sergio Callejas Rebeca Álvarez Alberto Benguria Ana Dopazo |
| author_sort | Sergio Callejas |
| collection | DOAJ |
| description | Protocol Summary We have developed automatic genomics NGS (AG-NGS), a computing application that allows an open liquid handling platform to be transformed into a library preparation station without losing the potential of an open platform. Implementation of AG-NGS does not require programming experience, and the application has also been designed to minimize implementation costs. Automated library preparation with AG-NGS generated high-quality libraries from different samples, demonstrating its efficiency, and all quality control parameters fell within the range of optimal values. |
| format | Article |
| id | doaj-art-f4bce2d8b95f4043be18fa2edbb7b853 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-f4bce2d8b95f4043be18fa2edbb7b8532025-08-20T02:25:58ZengTaylor & Francis GroupBioTechniques0736-62051940-98182017-01-01621xvi10.2144/000114504AG-NGS: A powerful and user-friendly computing application for the semi-automated preparation of next-generation sequencing libraries using open liquid handling platformsSergio Callejas0Rebeca Álvarez1Alberto Benguria2Ana Dopazo31Spanish National Center for Cardiovascular Research (CNIC), Madrid, Spain1Spanish National Center for Cardiovascular Research (CNIC), Madrid, Spain1Spanish National Center for Cardiovascular Research (CNIC), Madrid, Spain1Spanish National Center for Cardiovascular Research (CNIC), Madrid, SpainProtocol Summary We have developed automatic genomics NGS (AG-NGS), a computing application that allows an open liquid handling platform to be transformed into a library preparation station without losing the potential of an open platform. Implementation of AG-NGS does not require programming experience, and the application has also been designed to minimize implementation costs. Automated library preparation with AG-NGS generated high-quality libraries from different samples, demonstrating its efficiency, and all quality control parameters fell within the range of optimal values.https://www.future-science.com/doi/10.2144/000114504 |
| spellingShingle | Sergio Callejas Rebeca Álvarez Alberto Benguria Ana Dopazo AG-NGS: A powerful and user-friendly computing application for the semi-automated preparation of next-generation sequencing libraries using open liquid handling platforms BioTechniques |
| title | AG-NGS: A powerful and user-friendly computing application for the semi-automated preparation of next-generation sequencing libraries using open liquid handling platforms |
| title_full | AG-NGS: A powerful and user-friendly computing application for the semi-automated preparation of next-generation sequencing libraries using open liquid handling platforms |
| title_fullStr | AG-NGS: A powerful and user-friendly computing application for the semi-automated preparation of next-generation sequencing libraries using open liquid handling platforms |
| title_full_unstemmed | AG-NGS: A powerful and user-friendly computing application for the semi-automated preparation of next-generation sequencing libraries using open liquid handling platforms |
| title_short | AG-NGS: A powerful and user-friendly computing application for the semi-automated preparation of next-generation sequencing libraries using open liquid handling platforms |
| title_sort | ag ngs a powerful and user friendly computing application for the semi automated preparation of next generation sequencing libraries using open liquid handling platforms |
| url | https://www.future-science.com/doi/10.2144/000114504 |
| work_keys_str_mv | AT sergiocallejas agngsapowerfulanduserfriendlycomputingapplicationforthesemiautomatedpreparationofnextgenerationsequencinglibrariesusingopenliquidhandlingplatforms AT rebecaalvarez agngsapowerfulanduserfriendlycomputingapplicationforthesemiautomatedpreparationofnextgenerationsequencinglibrariesusingopenliquidhandlingplatforms AT albertobenguria agngsapowerfulanduserfriendlycomputingapplicationforthesemiautomatedpreparationofnextgenerationsequencinglibrariesusingopenliquidhandlingplatforms AT anadopazo agngsapowerfulanduserfriendlycomputingapplicationforthesemiautomatedpreparationofnextgenerationsequencinglibrariesusingopenliquidhandlingplatforms |