Generation of heterozygous KCNA2 knockout induced pluripotent stem cell line
The KCNA2 gene is the voltage-gated ion channel from both functional and structural perspectives. KCNA2 is involved in diverse functions including the regulation of neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contrac...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506124002940 |
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| author | Wei shan Xiaomeng Cui Dayan Wang Baiqiang Wang Xiangge Guo Xumeng Wang Jiaxuan Wang Yanting Li Guipeng An Qian Ren |
| author_facet | Wei shan Xiaomeng Cui Dayan Wang Baiqiang Wang Xiangge Guo Xumeng Wang Jiaxuan Wang Yanting Li Guipeng An Qian Ren |
| author_sort | Wei shan |
| collection | DOAJ |
| description | The KCNA2 gene is the voltage-gated ion channel from both functional and structural perspectives. KCNA2 is involved in diverse functions including the regulation of neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. To investigate the relevant pathophysiological mechanisms, we generated heterozygous KCNA2 knockout human induced pluripotent stem cell (iPSC) line via CRISPR/Cas9 gene editing. The generated iPSCs had a normal karyotype, were free of genetically integrated epitaxial plasmids, expressed pluripotency markers, and maintained trilineage differentiation potential. |
| format | Article |
| id | doaj-art-f4bb966ee84c4e00bd2a8798eed4475c |
| institution | OA Journals |
| issn | 1873-5061 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj-art-f4bb966ee84c4e00bd2a8798eed4475c2025-08-20T01:59:35ZengElsevierStem Cell Research1873-50612024-12-018110359610.1016/j.scr.2024.103596Generation of heterozygous KCNA2 knockout induced pluripotent stem cell lineWei shan0Xiaomeng Cui1Dayan Wang2Baiqiang Wang3Xiangge Guo4Xumeng Wang5Jiaxuan Wang6Yanting Li7Guipeng An8Qian Ren9Department of neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China; National Center for Clinical Medicine of Neurological Diseases, Beijing 10070, ChinaDepartment of neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China; Department of Neurology, Zibo City Hospital Combined of Traditional Chinese and Western Medicine, Zibo 255000, ChinaDepartment of neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100070, China; Department of Neurology, Avant-garde Hospital of Jilin Province, Changchun 130012, ChinaKey Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese Ministry of Health and Chinese Academy of Medical Sciences, The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, Jinan 100034, China; Department of Cardiology, Qilu Hospital of Shandong University, Jinan 100034, ChinaDepartment of Human Anatomy, Hebei Medical University, Shijiazhuang 050017, China; International Cooperation Laboratory of Stem Cell Research, Institute of Medical and Health Science, Hebei Medical University, Shijiazhuang 050017, ChinaDepartment of Human Anatomy, Hebei Medical University, Shijiazhuang 050017, China; International Cooperation Laboratory of Stem Cell Research, Institute of Medical and Health Science, Hebei Medical University, Shijiazhuang 050017, ChinaDepartment of Human Anatomy, Hebei Medical University, Shijiazhuang 050017, China; International Cooperation Laboratory of Stem Cell Research, Institute of Medical and Health Science, Hebei Medical University, Shijiazhuang 050017, ChinaDepartment of Human Anatomy, Hebei Medical University, Shijiazhuang 050017, China; International Cooperation Laboratory of Stem Cell Research, Institute of Medical and Health Science, Hebei Medical University, Shijiazhuang 050017, ChinaKey Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese Ministry of Health and Chinese Academy of Medical Sciences, The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Qilu Hospital of Shandong University, Jinan 100034, China; Department of Cardiology, Qilu Hospital of Shandong University, Jinan 100034, China; Corresponding authors.Department of Human Anatomy, Hebei Medical University, Shijiazhuang 050017, China; International Cooperation Laboratory of Stem Cell Research, Institute of Medical and Health Science, Hebei Medical University, Shijiazhuang 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Shijiazhuang 050017, China; Corresponding authors.The KCNA2 gene is the voltage-gated ion channel from both functional and structural perspectives. KCNA2 is involved in diverse functions including the regulation of neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. To investigate the relevant pathophysiological mechanisms, we generated heterozygous KCNA2 knockout human induced pluripotent stem cell (iPSC) line via CRISPR/Cas9 gene editing. The generated iPSCs had a normal karyotype, were free of genetically integrated epitaxial plasmids, expressed pluripotency markers, and maintained trilineage differentiation potential.http://www.sciencedirect.com/science/article/pii/S1873506124002940 |
| spellingShingle | Wei shan Xiaomeng Cui Dayan Wang Baiqiang Wang Xiangge Guo Xumeng Wang Jiaxuan Wang Yanting Li Guipeng An Qian Ren Generation of heterozygous KCNA2 knockout induced pluripotent stem cell line Stem Cell Research |
| title | Generation of heterozygous KCNA2 knockout induced pluripotent stem cell line |
| title_full | Generation of heterozygous KCNA2 knockout induced pluripotent stem cell line |
| title_fullStr | Generation of heterozygous KCNA2 knockout induced pluripotent stem cell line |
| title_full_unstemmed | Generation of heterozygous KCNA2 knockout induced pluripotent stem cell line |
| title_short | Generation of heterozygous KCNA2 knockout induced pluripotent stem cell line |
| title_sort | generation of heterozygous kcna2 knockout induced pluripotent stem cell line |
| url | http://www.sciencedirect.com/science/article/pii/S1873506124002940 |
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