Elucidating the effects of manganese on the growth and cadmium accumulation of OsNRAMP5 mutant rice
Low-cadmium (Cd)-accumulating rice (Oryza sativa L.) cultivated by OsNRAMP5 mutation is a promising approach for ensuring food safety and human health. However, the impacts of reduced manganese (Mn) uptake caused by OsNRAMP5 mutation on rice growth and Cd accumulation under varying Mn levels remains...
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| Language: | English |
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Elsevier
2025-02-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S014765132500199X |
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| author | Xian’an Yu Qiuqin Zhou Xin-Yuan Huang Mingxue Chen Jiawen Zhou Tong Zhou Pengjie Hu Xia Pan Miaomiao Cheng Yongming Luo Peter Christie Longhua Wu |
| author_facet | Xian’an Yu Qiuqin Zhou Xin-Yuan Huang Mingxue Chen Jiawen Zhou Tong Zhou Pengjie Hu Xia Pan Miaomiao Cheng Yongming Luo Peter Christie Longhua Wu |
| author_sort | Xian’an Yu |
| collection | DOAJ |
| description | Low-cadmium (Cd)-accumulating rice (Oryza sativa L.) cultivated by OsNRAMP5 mutation is a promising approach for ensuring food safety and human health. However, the impacts of reduced manganese (Mn) uptake caused by OsNRAMP5 mutation on rice growth and Cd accumulation under varying Mn levels remains unknown. Here, hydroponics and pot experiments were used to investigate the growth and Cd accumulation of an OsNRAMP5 mutant rice, Zhong’an 7 (ZA7), and the wild type, Zhongzao 35 (ZZ35), under different Mn supply conditions, and the Mn thresholds for ZA7 were determined. There was lower biomass of ZA7 than ZZ35 under hydroponic conditions with 0–0.05 μM Mn supply, and this was alleviated at Mn concentrations ranging from 1 to 200 μM. In pot Experiment I the grain yields of ZA7 in five soils increased from 5.92 to 15.0 g pot−1 as the inherent soil available Mn ranged from 3.17 to 400 mg kg−1. In pot Experiment II the exogenous MnSO4 significantly increased ZA7 grain yields by 135–173 % and reduced grain Cd concentrations by 50.3–72.9 % (p < 0.05). Furthermore, both hydroponic and pot experiments show that Cd concentrations in ZA7 were significantly lower than those in ZZ35, and decreased further with increasing Mn supply. Finally, the relationship between soil available-Mn (diethylene-triamine-pentaacetic acid-extractable, DTPA-Mn), which is directly related to plant Mn uptake, and ZA7 grain yields fitted well. The optimum DTPA-Mn content for maximum yield of ZA7 occurred at 263 mg kg−1 and the minimum DTPA-Mn threshold ensuring 90 % of the optimum grain yield was 165 mg kg−1. Thus, the results demonstrated the greater Mn-deficiency sensitivity and higher Mn requirement of ZA7 compared to ZZ35, shedding new light on the wider application of OsNRAMP5 mutant rice under different Mn conditions. |
| format | Article |
| id | doaj-art-f4b8b304153b42548716803c87d8a842 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-f4b8b304153b42548716803c87d8a8422025-02-08T04:59:23ZengElsevierEcotoxicology and Environmental Safety0147-65132025-02-01291117863Elucidating the effects of manganese on the growth and cadmium accumulation of OsNRAMP5 mutant riceXian’an Yu0Qiuqin Zhou1Xin-Yuan Huang2Mingxue Chen3Jiawen Zhou4Tong Zhou5Pengjie Hu6Xia Pan7Miaomiao Cheng8Yongming Luo9Peter Christie10Longhua Wu11State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 211135, China; University of the Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 211135, ChinaState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, ChinaRice Product Quality Supervision and Inspection Center, China National Rice Research Institute, Hangzhou 310006, ChinaState Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 211135, China; Corresponding authors.State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 211135, ChinaState Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 211135, ChinaCollege of Optoelectronic Manufacturing, Zhejiang Industry & Trade Vocational College, Wenzhou 325003, ChinaCenter for Sustainable Farming Systems, Food Futures Institute, Murdoch University, Perth, Western Australia, AustraliaState Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 211135, ChinaState Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 211135, ChinaState Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 211135, China; Corresponding authors.Low-cadmium (Cd)-accumulating rice (Oryza sativa L.) cultivated by OsNRAMP5 mutation is a promising approach for ensuring food safety and human health. However, the impacts of reduced manganese (Mn) uptake caused by OsNRAMP5 mutation on rice growth and Cd accumulation under varying Mn levels remains unknown. Here, hydroponics and pot experiments were used to investigate the growth and Cd accumulation of an OsNRAMP5 mutant rice, Zhong’an 7 (ZA7), and the wild type, Zhongzao 35 (ZZ35), under different Mn supply conditions, and the Mn thresholds for ZA7 were determined. There was lower biomass of ZA7 than ZZ35 under hydroponic conditions with 0–0.05 μM Mn supply, and this was alleviated at Mn concentrations ranging from 1 to 200 μM. In pot Experiment I the grain yields of ZA7 in five soils increased from 5.92 to 15.0 g pot−1 as the inherent soil available Mn ranged from 3.17 to 400 mg kg−1. In pot Experiment II the exogenous MnSO4 significantly increased ZA7 grain yields by 135–173 % and reduced grain Cd concentrations by 50.3–72.9 % (p < 0.05). Furthermore, both hydroponic and pot experiments show that Cd concentrations in ZA7 were significantly lower than those in ZZ35, and decreased further with increasing Mn supply. Finally, the relationship between soil available-Mn (diethylene-triamine-pentaacetic acid-extractable, DTPA-Mn), which is directly related to plant Mn uptake, and ZA7 grain yields fitted well. The optimum DTPA-Mn content for maximum yield of ZA7 occurred at 263 mg kg−1 and the minimum DTPA-Mn threshold ensuring 90 % of the optimum grain yield was 165 mg kg−1. Thus, the results demonstrated the greater Mn-deficiency sensitivity and higher Mn requirement of ZA7 compared to ZZ35, shedding new light on the wider application of OsNRAMP5 mutant rice under different Mn conditions.http://www.sciencedirect.com/science/article/pii/S014765132500199XOryza sativaOsNRAMP5 mutationManganeseYieldCadmium uptake |
| spellingShingle | Xian’an Yu Qiuqin Zhou Xin-Yuan Huang Mingxue Chen Jiawen Zhou Tong Zhou Pengjie Hu Xia Pan Miaomiao Cheng Yongming Luo Peter Christie Longhua Wu Elucidating the effects of manganese on the growth and cadmium accumulation of OsNRAMP5 mutant rice Ecotoxicology and Environmental Safety Oryza sativa OsNRAMP5 mutation Manganese Yield Cadmium uptake |
| title | Elucidating the effects of manganese on the growth and cadmium accumulation of OsNRAMP5 mutant rice |
| title_full | Elucidating the effects of manganese on the growth and cadmium accumulation of OsNRAMP5 mutant rice |
| title_fullStr | Elucidating the effects of manganese on the growth and cadmium accumulation of OsNRAMP5 mutant rice |
| title_full_unstemmed | Elucidating the effects of manganese on the growth and cadmium accumulation of OsNRAMP5 mutant rice |
| title_short | Elucidating the effects of manganese on the growth and cadmium accumulation of OsNRAMP5 mutant rice |
| title_sort | elucidating the effects of manganese on the growth and cadmium accumulation of osnramp5 mutant rice |
| topic | Oryza sativa OsNRAMP5 mutation Manganese Yield Cadmium uptake |
| url | http://www.sciencedirect.com/science/article/pii/S014765132500199X |
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