Phenylethanol Glycoside from <i>Cistanche tubulosa</i> Attenuates BSA-Induced Liver Fibrosis in Rats by Modulating the Gut Microbiota–Liver Axis
This study aimed to investigate the effect of phenylethanol glycoside from <i>Cistanche tubulosa</i> (CPhGs) on the prevention of bovine serum albumin (BSA)-induced hepatic fibrosis in rats. Investigation of the mechanisms of the anti-hepatic fibrosis effect was focused on CPhGs’ influen...
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MDPI AG
2024-08-01
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| author | Xinxin Qi Hongguang Sun Jincun Liu Meili Cong Xinxuan Zhang Yuxin Yan Zhaolin Xia Tao Liu Jun Zhao |
| author_facet | Xinxin Qi Hongguang Sun Jincun Liu Meili Cong Xinxuan Zhang Yuxin Yan Zhaolin Xia Tao Liu Jun Zhao |
| author_sort | Xinxin Qi |
| collection | DOAJ |
| description | This study aimed to investigate the effect of phenylethanol glycoside from <i>Cistanche tubulosa</i> (CPhGs) on the prevention of bovine serum albumin (BSA)-induced hepatic fibrosis in rats. Investigation of the mechanisms of the anti-hepatic fibrosis effect was focused on CPhGs’ influence on the “gut–liver” regulation, including the gut microbiota, intestinal barrier, systemic lipopolysaccharide (LPS) concentration, and LPS-related signaling pathway. The results show that CPhGs restored the diversity of gut microbiota, increased the relative abundance of <i>Bacteroidetes</i>, and decreased the relative abundance of <i>Firmicutes</i> and <i>Proteobacteria</i> in the fibrotic rats. In addition, CPhGs promoted the enrichment of probiotics such as <i>Blautia</i>, <i>Oscillospira</i>, <i>Ruminococcus</i>, <i>Odoribacter</i>, <i>Bacteroides</i>, and <i>Parabacteroides</i> in intestines of these rats. Furthermore, CPhGs reduced histopathological injury in the intestine and restored the tight junctions of the intestine by increasing the expression of ZO-1, occludin, and E-cadherin. CPhGs efficiently reduced serum LPS and liver lipopolysaccharide-binding protein (LBP) levels and inhibited the LPS-TLR4/MyD88/NF-κB pathway, which is related to protein expression in the liver. Correlation analysis confirmed that these beneficial bacteria were negatively associated with pathological damage, while LPS and harmful bacteria were positively associated with liver injury. Our fecal microbiota transplantation (FMT) experiment confirmed that gut microbiota is an important part of disease progression and that CPhGs is useful for the prevention and treatment of hepatic fibrosis. Our data demonstrate that the anti-hepatic fibrosis mechanism of CPhGs was mediated by regulation of the “gut–liver” axis. These results can stimulate consideration for its use in clinical practices. |
| format | Article |
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| institution | OA Journals |
| issn | 1424-8247 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | MDPI AG |
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| series | Pharmaceuticals |
| spelling | doaj-art-f4ac2a62bb204d328fdb3df78b72fb0f2025-08-20T01:55:46ZengMDPI AGPharmaceuticals1424-82472024-08-01179114910.3390/ph17091149Phenylethanol Glycoside from <i>Cistanche tubulosa</i> Attenuates BSA-Induced Liver Fibrosis in Rats by Modulating the Gut Microbiota–Liver AxisXinxin Qi0Hongguang Sun1Jincun Liu2Meili Cong3Xinxuan Zhang4Yuxin Yan5Zhaolin Xia6Tao Liu7Jun Zhao8School of Public Health, Xinjiang Medical University, Urumqi 830011, ChinaSchool of Public Health, Xinjiang Medical University, Urumqi 830011, ChinaSchool of Public Health, Xinjiang Medical University, Urumqi 830011, ChinaSchool of Public Health, Xinjiang Medical University, Urumqi 830011, ChinaSchool of Public Health, Xinjiang Medical University, Urumqi 830011, ChinaSchool of Public Health, Xinjiang Medical University, Urumqi 830011, ChinaSchool of Public Health, Xinjiang Medical University, Urumqi 830011, ChinaSchool of Public Health, Xinjiang Medical University, Urumqi 830011, ChinaSchool of Public Health, Xinjiang Medical University, Urumqi 830011, ChinaThis study aimed to investigate the effect of phenylethanol glycoside from <i>Cistanche tubulosa</i> (CPhGs) on the prevention of bovine serum albumin (BSA)-induced hepatic fibrosis in rats. Investigation of the mechanisms of the anti-hepatic fibrosis effect was focused on CPhGs’ influence on the “gut–liver” regulation, including the gut microbiota, intestinal barrier, systemic lipopolysaccharide (LPS) concentration, and LPS-related signaling pathway. The results show that CPhGs restored the diversity of gut microbiota, increased the relative abundance of <i>Bacteroidetes</i>, and decreased the relative abundance of <i>Firmicutes</i> and <i>Proteobacteria</i> in the fibrotic rats. In addition, CPhGs promoted the enrichment of probiotics such as <i>Blautia</i>, <i>Oscillospira</i>, <i>Ruminococcus</i>, <i>Odoribacter</i>, <i>Bacteroides</i>, and <i>Parabacteroides</i> in intestines of these rats. Furthermore, CPhGs reduced histopathological injury in the intestine and restored the tight junctions of the intestine by increasing the expression of ZO-1, occludin, and E-cadherin. CPhGs efficiently reduced serum LPS and liver lipopolysaccharide-binding protein (LBP) levels and inhibited the LPS-TLR4/MyD88/NF-κB pathway, which is related to protein expression in the liver. Correlation analysis confirmed that these beneficial bacteria were negatively associated with pathological damage, while LPS and harmful bacteria were positively associated with liver injury. Our fecal microbiota transplantation (FMT) experiment confirmed that gut microbiota is an important part of disease progression and that CPhGs is useful for the prevention and treatment of hepatic fibrosis. Our data demonstrate that the anti-hepatic fibrosis mechanism of CPhGs was mediated by regulation of the “gut–liver” axis. These results can stimulate consideration for its use in clinical practices.https://www.mdpi.com/1424-8247/17/9/1149<i>Cistanche tubulosa</i>phenylethanol glycosidesliver fibrosisgut microbiotafecal microbiota transplantation |
| spellingShingle | Xinxin Qi Hongguang Sun Jincun Liu Meili Cong Xinxuan Zhang Yuxin Yan Zhaolin Xia Tao Liu Jun Zhao Phenylethanol Glycoside from <i>Cistanche tubulosa</i> Attenuates BSA-Induced Liver Fibrosis in Rats by Modulating the Gut Microbiota–Liver Axis Pharmaceuticals <i>Cistanche tubulosa</i> phenylethanol glycosides liver fibrosis gut microbiota fecal microbiota transplantation |
| title | Phenylethanol Glycoside from <i>Cistanche tubulosa</i> Attenuates BSA-Induced Liver Fibrosis in Rats by Modulating the Gut Microbiota–Liver Axis |
| title_full | Phenylethanol Glycoside from <i>Cistanche tubulosa</i> Attenuates BSA-Induced Liver Fibrosis in Rats by Modulating the Gut Microbiota–Liver Axis |
| title_fullStr | Phenylethanol Glycoside from <i>Cistanche tubulosa</i> Attenuates BSA-Induced Liver Fibrosis in Rats by Modulating the Gut Microbiota–Liver Axis |
| title_full_unstemmed | Phenylethanol Glycoside from <i>Cistanche tubulosa</i> Attenuates BSA-Induced Liver Fibrosis in Rats by Modulating the Gut Microbiota–Liver Axis |
| title_short | Phenylethanol Glycoside from <i>Cistanche tubulosa</i> Attenuates BSA-Induced Liver Fibrosis in Rats by Modulating the Gut Microbiota–Liver Axis |
| title_sort | phenylethanol glycoside from i cistanche tubulosa i attenuates bsa induced liver fibrosis in rats by modulating the gut microbiota liver axis |
| topic | <i>Cistanche tubulosa</i> phenylethanol glycosides liver fibrosis gut microbiota fecal microbiota transplantation |
| url | https://www.mdpi.com/1424-8247/17/9/1149 |
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