Antibacterial, antifungal, and antibiofilm activities of biogenic zinc nanoparticles against pathogenic microorganisms
IntroductionThe increasing resistance to antimicrobial drugs has prompted global efforts to combat pathogenic bacteria and fungi. The World Health Organization’s recent report underscores the urgent need for innovative antimicrobial strategies to address infections caused by Staphylococcus aureus, E...
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1545119/full |
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| author | Eliana Daniela Lopez Venditti Eliana Daniela Lopez Venditti Karina Fernanda Crespo Andrada Karina Fernanda Crespo Andrada Pamela Soledad Bustos Manuela Maldonado Torales Manuela Maldonado Torales Iván Manrrique Hughes Iván Manrrique Hughes María Gabriela Paraje María Gabriela Paraje Natalia Guiñazú Natalia Guiñazú |
| author_facet | Eliana Daniela Lopez Venditti Eliana Daniela Lopez Venditti Karina Fernanda Crespo Andrada Karina Fernanda Crespo Andrada Pamela Soledad Bustos Manuela Maldonado Torales Manuela Maldonado Torales Iván Manrrique Hughes Iván Manrrique Hughes María Gabriela Paraje María Gabriela Paraje Natalia Guiñazú Natalia Guiñazú |
| author_sort | Eliana Daniela Lopez Venditti |
| collection | DOAJ |
| description | IntroductionThe increasing resistance to antimicrobial drugs has prompted global efforts to combat pathogenic bacteria and fungi. The World Health Organization’s recent report underscores the urgent need for innovative antimicrobial strategies to address infections caused by Staphylococcus aureus, Escherichia coli, Candida albicans, and Candida tropicalis. This study presents a comparative evaluation of the effects of biogenically synthesized zinc nanoparticles (ZnNPs) from Pseudomonas aeruginosa, highlighting their effectiveness against both planktonic and sessile forms of these tested pathogens.MethodsThe antimicrobial effects were assessed using the Kirby-Bauer disk diffusion method, broth microdilution, and time-kill assays. Biofilm formation and eradication were evaluated through crystal violet staining, resazurin assays, and colony-forming unit quantification. Additionally, the oxidative and nitrosative stress toxicity mechanisms triggered by ZnNPs, particularly those related to cellular stress, were investigated.ResultsThe results demonstrated that ZnNPs exhibit concentration-dependent inhibitory effects on both prokaryotic and eukaryotic microorganisms. ZnNPs inhibit biofilm formation by up to 50% in E. coli and yeast species, and up to 80% in S. aureus.DiscussionThese antibiofilm activities were attributed to disruptions in cellular stress metabolism, primarily driven by nitrosative stress through enhanced production of reactive nitrogen intermediates. ZnNPs synthesized through green methods offer significant advantages due to their biocompatibility and potential biomedical applications. These findings advance our understanding of ZnNPs in combating biofilm-associated infections, offering promising strategies to address pathogenic bacteria and fungi, which pose a critical threat to global health. |
| format | Article |
| id | doaj-art-f4ac19d08ca64c529e99a6cee10453fa |
| institution | DOAJ |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-f4ac19d08ca64c529e99a6cee10453fa2025-08-20T03:11:46ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-07-011510.3389/fcimb.2025.15451191545119Antibacterial, antifungal, and antibiofilm activities of biogenic zinc nanoparticles against pathogenic microorganismsEliana Daniela Lopez Venditti0Eliana Daniela Lopez Venditti1Karina Fernanda Crespo Andrada2Karina Fernanda Crespo Andrada3Pamela Soledad Bustos4Manuela Maldonado Torales5Manuela Maldonado Torales6Iván Manrrique Hughes7Iván Manrrique Hughes8María Gabriela Paraje9María Gabriela Paraje10Natalia Guiñazú11Natalia Guiñazú12Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue (CITAAC), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Neuquén, ArgentinaDepartamento de Ciencias del Ambiente y la Salud, Facultad de Ciencias del Ambiente y la Salud, Universidad Nacional del Comahue, Neuquén, ArgentinaCátedra de Microbiología, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba, ArgentinaInstituto Multidisciplinario de Biología Vegetal (IMBIV), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Córdoba, ArgentinaDepartamento de Ciencias Farmacéuticas, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre y Medina Allende, Córdoba, ArgentinaCátedra de Microbiología, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba, ArgentinaInstituto Multidisciplinario de Biología Vegetal (IMBIV), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Córdoba, ArgentinaCátedra de Microbiología, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba, ArgentinaInstituto Multidisciplinario de Biología Vegetal (IMBIV), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Córdoba, ArgentinaCátedra de Microbiología, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba, ArgentinaInstituto Multidisciplinario de Biología Vegetal (IMBIV), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Córdoba, ArgentinaCentro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue (CITAAC), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Neuquén, ArgentinaDepartamento de Ciencias del Ambiente y la Salud, Facultad de Ciencias del Ambiente y la Salud, Universidad Nacional del Comahue, Neuquén, ArgentinaIntroductionThe increasing resistance to antimicrobial drugs has prompted global efforts to combat pathogenic bacteria and fungi. The World Health Organization’s recent report underscores the urgent need for innovative antimicrobial strategies to address infections caused by Staphylococcus aureus, Escherichia coli, Candida albicans, and Candida tropicalis. This study presents a comparative evaluation of the effects of biogenically synthesized zinc nanoparticles (ZnNPs) from Pseudomonas aeruginosa, highlighting their effectiveness against both planktonic and sessile forms of these tested pathogens.MethodsThe antimicrobial effects were assessed using the Kirby-Bauer disk diffusion method, broth microdilution, and time-kill assays. Biofilm formation and eradication were evaluated through crystal violet staining, resazurin assays, and colony-forming unit quantification. Additionally, the oxidative and nitrosative stress toxicity mechanisms triggered by ZnNPs, particularly those related to cellular stress, were investigated.ResultsThe results demonstrated that ZnNPs exhibit concentration-dependent inhibitory effects on both prokaryotic and eukaryotic microorganisms. ZnNPs inhibit biofilm formation by up to 50% in E. coli and yeast species, and up to 80% in S. aureus.DiscussionThese antibiofilm activities were attributed to disruptions in cellular stress metabolism, primarily driven by nitrosative stress through enhanced production of reactive nitrogen intermediates. ZnNPs synthesized through green methods offer significant advantages due to their biocompatibility and potential biomedical applications. These findings advance our understanding of ZnNPs in combating biofilm-associated infections, offering promising strategies to address pathogenic bacteria and fungi, which pose a critical threat to global health.https://www.frontiersin.org/articles/10.3389/fcimb.2025.1545119/fullbiogenic zinc nanoparticlesantibacterial activityantifungal activitybiofilm associated infectionsoxidative stressnitrosative stress |
| spellingShingle | Eliana Daniela Lopez Venditti Eliana Daniela Lopez Venditti Karina Fernanda Crespo Andrada Karina Fernanda Crespo Andrada Pamela Soledad Bustos Manuela Maldonado Torales Manuela Maldonado Torales Iván Manrrique Hughes Iván Manrrique Hughes María Gabriela Paraje María Gabriela Paraje Natalia Guiñazú Natalia Guiñazú Antibacterial, antifungal, and antibiofilm activities of biogenic zinc nanoparticles against pathogenic microorganisms Frontiers in Cellular and Infection Microbiology biogenic zinc nanoparticles antibacterial activity antifungal activity biofilm associated infections oxidative stress nitrosative stress |
| title | Antibacterial, antifungal, and antibiofilm activities of biogenic zinc nanoparticles against pathogenic microorganisms |
| title_full | Antibacterial, antifungal, and antibiofilm activities of biogenic zinc nanoparticles against pathogenic microorganisms |
| title_fullStr | Antibacterial, antifungal, and antibiofilm activities of biogenic zinc nanoparticles against pathogenic microorganisms |
| title_full_unstemmed | Antibacterial, antifungal, and antibiofilm activities of biogenic zinc nanoparticles against pathogenic microorganisms |
| title_short | Antibacterial, antifungal, and antibiofilm activities of biogenic zinc nanoparticles against pathogenic microorganisms |
| title_sort | antibacterial antifungal and antibiofilm activities of biogenic zinc nanoparticles against pathogenic microorganisms |
| topic | biogenic zinc nanoparticles antibacterial activity antifungal activity biofilm associated infections oxidative stress nitrosative stress |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1545119/full |
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