Pan-cancer analysis of CLDN1 and its functional study in gastric cancer
Objective This study employs bioinformatics methods to analyze the expression of Claudin 1 (CLDN1) across various types of cancer and its functional role in gastric cancer. Methods The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases were used to evaluate the expression pat...
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| Format: | Article |
| Language: | zho |
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Lanzhou University Press
2024-12-01
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| Series: | 生物医学转化 |
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| Online Access: | http://swyxzh.ijournals.cn/swyxzh/article/html/20240409 |
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| _version_ | 1841543730127962112 |
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| author | Zhu Weijing Zhang Jiaxing Wei Yucai Yang Shengrui Wang Wenxuan Li Yumin |
| author_facet | Zhu Weijing Zhang Jiaxing Wei Yucai Yang Shengrui Wang Wenxuan Li Yumin |
| author_sort | Zhu Weijing |
| collection | DOAJ |
| description | Objective This study employs bioinformatics methods to analyze the expression of Claudin 1 (CLDN1) across various types of cancer and its functional role in gastric cancer. Methods The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases were used to evaluate the expression patterns of CLDN1 in various cancer types and their association with clinical pathology, patient prognosis, and immune characteristics. Western blotting, Polymerase Chain Reaction(PCR), and cell function assays were used to examine the expression and function of CLDN1 in gastric cancer cells. Results CLDN1 was significantly upregulated in 13 types of tumors and downregulated in 7, with a strong correlation to tumor stage and grade. It demonstrated good diagnostic potential and was associated with poor prognosis in 8 cancer types. Additionally, CLDN1 showed the highest mutation rate in lung squamous cell carcinoma. Immune analysis revealed significant correlations between CLDN1 expression and immune infiltration, immune checkpoint gene expression, tumor mutation burden, and microsatellite instability across most cancer types. CLDN1 was highly expressed in MKN45 cells, and its knockdown significantly reduced cell proliferation and migration. Conclusion CLDN1 exhibits significant expression heterogeneity across various cancers, with strong associations with diagnosis, prognosis, clinical characteristics, and immune profiling. It represents a potential diagnostic marker and therapeutic target in cancer treatment. |
| format | Article |
| id | doaj-art-f4a06421c3204430b1b5a7e1b20b03e7 |
| institution | Kabale University |
| issn | 2096-8965 |
| language | zho |
| publishDate | 2024-12-01 |
| publisher | Lanzhou University Press |
| record_format | Article |
| series | 生物医学转化 |
| spelling | doaj-art-f4a06421c3204430b1b5a7e1b20b03e72025-01-13T07:29:08ZzhoLanzhou University Press生物医学转化2096-89652024-12-0154708110.12287/j.issn.2096-8965.20240409Pan-cancer analysis of CLDN1 and its functional study in gastric cancerZhu Weijing0Zhang Jiaxing1Wei Yucai2Yang Shengrui3Wang Wenxuan4Li Yumin5Department of General Surgery, The Second Hospital & Clinical Medical School, Lanzhou University,Lanzhou 730030 , Gansu, ChinaDepartment of General Surgery, The Second Hospital & Clinical Medical School, Lanzhou University,Lanzhou 730030 , Gansu, ChinaDepartment of Surgical Oncology, The Second Hospital & Clinical Medical School, Lanzhou University,Lanzhou 730030 , Gansu, ChinaDepartment of General Surgery, The Second Hospital & Clinical Medical School, Lanzhou University,Lanzhou 730030 , Gansu, ChinaDepartment of General Surgery, The Second Hospital & Clinical Medical School, Lanzhou University,Lanzhou 730030 , Gansu, ChinaDepartment of General Surgery, The Second Hospital & Clinical Medical School, Lanzhou University,Lanzhou 730030 , Gansu, China ；Department of Surgical Oncology, The Second Hospital & Clinical Medical School, Lanzhou University,Lanzhou 730030 , Gansu, China ；Environmental Oncology Center, Lanzhou University, Lanzhou 730030 , Gansu, China ；Gansu Provincial Key Laboratory of Environmental Oncology, Lanzhou 730030 , Gansu, ChinaObjective This study employs bioinformatics methods to analyze the expression of Claudin 1 (CLDN1) across various types of cancer and its functional role in gastric cancer. Methods The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases were used to evaluate the expression patterns of CLDN1 in various cancer types and their association with clinical pathology, patient prognosis, and immune characteristics. Western blotting, Polymerase Chain Reaction(PCR), and cell function assays were used to examine the expression and function of CLDN1 in gastric cancer cells. Results CLDN1 was significantly upregulated in 13 types of tumors and downregulated in 7, with a strong correlation to tumor stage and grade. It demonstrated good diagnostic potential and was associated with poor prognosis in 8 cancer types. Additionally, CLDN1 showed the highest mutation rate in lung squamous cell carcinoma. Immune analysis revealed significant correlations between CLDN1 expression and immune infiltration, immune checkpoint gene expression, tumor mutation burden, and microsatellite instability across most cancer types. CLDN1 was highly expressed in MKN45 cells, and its knockdown significantly reduced cell proliferation and migration. Conclusion CLDN1 exhibits significant expression heterogeneity across various cancers, with strong associations with diagnosis, prognosis, clinical characteristics, and immune profiling. It represents a potential diagnostic marker and therapeutic target in cancer treatment.http://swyxzh.ijournals.cn/swyxzh/article/html/20240409claudin 1pan-cancer analysisexpression levelimmune infiltrationgastric cancer |
| spellingShingle | Zhu Weijing Zhang Jiaxing Wei Yucai Yang Shengrui Wang Wenxuan Li Yumin Pan-cancer analysis of CLDN1 and its functional study in gastric cancer 生物医学转化 claudin 1 pan-cancer analysis expression level immune infiltration gastric cancer |
| title | Pan-cancer analysis of CLDN1 and its functional study in gastric cancer |
| title_full | Pan-cancer analysis of CLDN1 and its functional study in gastric cancer |
| title_fullStr | Pan-cancer analysis of CLDN1 and its functional study in gastric cancer |
| title_full_unstemmed | Pan-cancer analysis of CLDN1 and its functional study in gastric cancer |
| title_short | Pan-cancer analysis of CLDN1 and its functional study in gastric cancer |
| title_sort | pan cancer analysis of cldn1 and its functional study in gastric cancer |
| topic | claudin 1 pan-cancer analysis expression level immune infiltration gastric cancer |
| url | http://swyxzh.ijournals.cn/swyxzh/article/html/20240409 |
| work_keys_str_mv | AT zhuweijing pancanceranalysisofcldn1anditsfunctionalstudyingastriccancer AT zhangjiaxing pancanceranalysisofcldn1anditsfunctionalstudyingastriccancer AT weiyucai pancanceranalysisofcldn1anditsfunctionalstudyingastriccancer AT yangshengrui pancanceranalysisofcldn1anditsfunctionalstudyingastriccancer AT wangwenxuan pancanceranalysisofcldn1anditsfunctionalstudyingastriccancer AT liyumin pancanceranalysisofcldn1anditsfunctionalstudyingastriccancer |