AEBP1 Promotes Glioblastoma Progression and Activates the Classical NF-κB Pathway
Objective. Our study was aimed at investigating the mechanistic consequences of the upregulation of adipocyte enhancer-binding protein 1 (AEBP1) in glioblastoma (GBM). Methods. The expression of AEBP1 in GBM was assessed by bioinformatics analysis and qRT-PCR; the effects of AEBP1 on GBM cell prolif...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Behavioural Neurology |
| Online Access: | http://dx.doi.org/10.1155/2020/8890452 |
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| _version_ | 1832565413826789376 |
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| author | Kai Guo Lei Song Jianyong Chang Peicheng Cao Qi Liu |
| author_facet | Kai Guo Lei Song Jianyong Chang Peicheng Cao Qi Liu |
| author_sort | Kai Guo |
| collection | DOAJ |
| description | Objective. Our study was aimed at investigating the mechanistic consequences of the upregulation of adipocyte enhancer-binding protein 1 (AEBP1) in glioblastoma (GBM). Methods. The expression of AEBP1 in GBM was assessed by bioinformatics analysis and qRT-PCR; the effects of AEBP1 on GBM cell proliferation, migration, invasion, and tumor growth in vitro and in vivo were detected by a CCK-8 assay, colony formation assay, scratch assay, Transwell assay, and subcutaneous tumor formation, respectively. The activation of related signaling pathways was monitored using western blot. Results. Tumor-related databases and bioinformatics analysis revealed that AEBP1 was highly expressed in GBM and indicated poor outcome of patients; its high expression that was also confirmed in GBM tissues and cell lines was closely related to the tumor size. The results of in vitro experiments showed that AEBP1 could significantly promote GBM cell proliferation, migration, and invasion; in vivo experiments suggested that AEBP1 could contribute to the growth of GBM tumors. AEBP1 could upregulate the level of IκBα phosphorylation, decrease IκBα expression, activate the NF-κB signaling pathway, and promote the expression of downstream oncogenes. Conclusion. Upregulated AEBP1 in GBM promotes GBM cell proliferation, migration, and invasion and facilitates tumor growth in vivo by activating the classical NF-κB pathway. |
| format | Article |
| id | doaj-art-f482d3aea4fc4cb7b2d2dd216496ac91 |
| institution | Kabale University |
| issn | 0953-4180 1875-8584 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Behavioural Neurology |
| spelling | doaj-art-f482d3aea4fc4cb7b2d2dd216496ac912025-02-03T01:07:57ZengWileyBehavioural Neurology0953-41801875-85842020-01-01202010.1155/2020/88904528890452AEBP1 Promotes Glioblastoma Progression and Activates the Classical NF-κB PathwayKai Guo0Lei Song1Jianyong Chang2Peicheng Cao3Qi Liu4Department of Neurosurgery, Weifang People’s Hospital, Weifang, Shandong, 261000, ChinaDepartment of Neurosurgery, Weifang People’s Hospital, Weifang, Shandong, 261000, ChinaDepartment of Neurosurgery, Weifang People’s Hospital, Weifang, Shandong, 261000, ChinaDepartment of Neurosurgery, Weifang People’s Hospital, Weifang, Shandong, 261000, ChinaDepartment of Neurosurgery, Weifang People’s Hospital, Weifang, Shandong, 261000, ChinaObjective. Our study was aimed at investigating the mechanistic consequences of the upregulation of adipocyte enhancer-binding protein 1 (AEBP1) in glioblastoma (GBM). Methods. The expression of AEBP1 in GBM was assessed by bioinformatics analysis and qRT-PCR; the effects of AEBP1 on GBM cell proliferation, migration, invasion, and tumor growth in vitro and in vivo were detected by a CCK-8 assay, colony formation assay, scratch assay, Transwell assay, and subcutaneous tumor formation, respectively. The activation of related signaling pathways was monitored using western blot. Results. Tumor-related databases and bioinformatics analysis revealed that AEBP1 was highly expressed in GBM and indicated poor outcome of patients; its high expression that was also confirmed in GBM tissues and cell lines was closely related to the tumor size. The results of in vitro experiments showed that AEBP1 could significantly promote GBM cell proliferation, migration, and invasion; in vivo experiments suggested that AEBP1 could contribute to the growth of GBM tumors. AEBP1 could upregulate the level of IκBα phosphorylation, decrease IκBα expression, activate the NF-κB signaling pathway, and promote the expression of downstream oncogenes. Conclusion. Upregulated AEBP1 in GBM promotes GBM cell proliferation, migration, and invasion and facilitates tumor growth in vivo by activating the classical NF-κB pathway.http://dx.doi.org/10.1155/2020/8890452 |
| spellingShingle | Kai Guo Lei Song Jianyong Chang Peicheng Cao Qi Liu AEBP1 Promotes Glioblastoma Progression and Activates the Classical NF-κB Pathway Behavioural Neurology |
| title | AEBP1 Promotes Glioblastoma Progression and Activates the Classical NF-κB Pathway |
| title_full | AEBP1 Promotes Glioblastoma Progression and Activates the Classical NF-κB Pathway |
| title_fullStr | AEBP1 Promotes Glioblastoma Progression and Activates the Classical NF-κB Pathway |
| title_full_unstemmed | AEBP1 Promotes Glioblastoma Progression and Activates the Classical NF-κB Pathway |
| title_short | AEBP1 Promotes Glioblastoma Progression and Activates the Classical NF-κB Pathway |
| title_sort | aebp1 promotes glioblastoma progression and activates the classical nf κb pathway |
| url | http://dx.doi.org/10.1155/2020/8890452 |
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