5-Aminolevulinic acid against strawberry Fusarium wilt: Bidirectional regulation of biocontrol agents and pathogens
Strawberry Fusarium wilt (SFW) is a systematic soil-borne disease caused by Fusarium oxysporum f. sp. fragaria (Fof), which infects the vascular bundles, blocking water and nutrient transport from roots to the aboveground. It is a severe pathogen which spreads rapidly and destroys strawberry product...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2024-11-01
|
| Series: | Horticultural Plant Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014123001784 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850202853397757952 |
|---|---|
| author | Hao Yang Jianting Zhang Haiwen Zhang Rongxiang Cao Donglan Tang Liangju Wang |
| author_facet | Hao Yang Jianting Zhang Haiwen Zhang Rongxiang Cao Donglan Tang Liangju Wang |
| author_sort | Hao Yang |
| collection | DOAJ |
| description | Strawberry Fusarium wilt (SFW) is a systematic soil-borne disease caused by Fusarium oxysporum f. sp. fragaria (Fof), which infects the vascular bundles, blocking water and nutrient transport from roots to the aboveground. It is a severe pathogen which spreads rapidly and destroys strawberry production. Finding a way to control this disease is of great scientific value and practical importance. In this study, three fungi were isolated from the vascular tissues of sick strawberries in the field. After DNA sequencing, they were identified as Fof, Aspergillus fumigatus and Trichoderma harzianum, respectively, among which the first two are pathogens and the third is a probiotic. All fungi were controlled by thiophanate-methyl (TM), a commercial fungicide. On PDA medium, 20 mg·L−1 5-aminolevulinic acid (ALA), a natural non-protein amino acid, promoted T. harzianum proliferation, but inhibited Fof and A. fumigatus. In confrontation test, the growth of Fof or A. fumigatus was inhibited by T. harzianum and exogenous ALA promoted T. harzianum growth but significantly inhibited the pathogen growth. When three species of fungi were separately or combinedly inoculated on healthy strawberry plants, T. harzianum promoted plant growth and development while Fof or A. fumigatus caused growth retardation, where Fof directly caused leaf yellowing and plant wilting. When the plants inoculated with different fungus were treated with ALA, the results turned out that ALA alleviated SFW symptoms by bidirectionally promoting T. harzianum proliferation and inhibiting Fof and A. fumigatus. Thus, ALA might be used in comprehensively controlling SFW in strawberry industry. |
| format | Article |
| id | doaj-art-f47b6ca52bc34061bd000f7caec86f2c |
| institution | OA Journals |
| issn | 2468-0141 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj-art-f47b6ca52bc34061bd000f7caec86f2c2025-08-20T02:11:38ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412024-11-011061349136110.1016/j.hpj.2023.02.0145-Aminolevulinic acid against strawberry Fusarium wilt: Bidirectional regulation of biocontrol agents and pathogensHao Yang0Jianting Zhang1Haiwen Zhang2Rongxiang Cao3Donglan Tang4Liangju Wang5College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 21095, ChinaCollege of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 21095, ChinaCollege of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 21095, ChinaInstitute of Nanjing Agricultural Sciences, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210046, ChinaInstitute of Nanjing Agricultural Sciences, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210046, ChinaCollege of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 21095, China; Corresponding author.Strawberry Fusarium wilt (SFW) is a systematic soil-borne disease caused by Fusarium oxysporum f. sp. fragaria (Fof), which infects the vascular bundles, blocking water and nutrient transport from roots to the aboveground. It is a severe pathogen which spreads rapidly and destroys strawberry production. Finding a way to control this disease is of great scientific value and practical importance. In this study, three fungi were isolated from the vascular tissues of sick strawberries in the field. After DNA sequencing, they were identified as Fof, Aspergillus fumigatus and Trichoderma harzianum, respectively, among which the first two are pathogens and the third is a probiotic. All fungi were controlled by thiophanate-methyl (TM), a commercial fungicide. On PDA medium, 20 mg·L−1 5-aminolevulinic acid (ALA), a natural non-protein amino acid, promoted T. harzianum proliferation, but inhibited Fof and A. fumigatus. In confrontation test, the growth of Fof or A. fumigatus was inhibited by T. harzianum and exogenous ALA promoted T. harzianum growth but significantly inhibited the pathogen growth. When three species of fungi were separately or combinedly inoculated on healthy strawberry plants, T. harzianum promoted plant growth and development while Fof or A. fumigatus caused growth retardation, where Fof directly caused leaf yellowing and plant wilting. When the plants inoculated with different fungus were treated with ALA, the results turned out that ALA alleviated SFW symptoms by bidirectionally promoting T. harzianum proliferation and inhibiting Fof and A. fumigatus. Thus, ALA might be used in comprehensively controlling SFW in strawberry industry.http://www.sciencedirect.com/science/article/pii/S24680141230017845-Aminolevulinic acidAntagonistic effectDisease controlFusarium oxysporum f. sp. fragaria (Fof)Strawberry Fusarium wilt (SFW)Trichoderma harzianum |
| spellingShingle | Hao Yang Jianting Zhang Haiwen Zhang Rongxiang Cao Donglan Tang Liangju Wang 5-Aminolevulinic acid against strawberry Fusarium wilt: Bidirectional regulation of biocontrol agents and pathogens Horticultural Plant Journal 5-Aminolevulinic acid Antagonistic effect Disease control Fusarium oxysporum f. sp. fragaria (Fof) Strawberry Fusarium wilt (SFW) Trichoderma harzianum |
| title | 5-Aminolevulinic acid against strawberry Fusarium wilt: Bidirectional regulation of biocontrol agents and pathogens |
| title_full | 5-Aminolevulinic acid against strawberry Fusarium wilt: Bidirectional regulation of biocontrol agents and pathogens |
| title_fullStr | 5-Aminolevulinic acid against strawberry Fusarium wilt: Bidirectional regulation of biocontrol agents and pathogens |
| title_full_unstemmed | 5-Aminolevulinic acid against strawberry Fusarium wilt: Bidirectional regulation of biocontrol agents and pathogens |
| title_short | 5-Aminolevulinic acid against strawberry Fusarium wilt: Bidirectional regulation of biocontrol agents and pathogens |
| title_sort | 5 aminolevulinic acid against strawberry fusarium wilt bidirectional regulation of biocontrol agents and pathogens |
| topic | 5-Aminolevulinic acid Antagonistic effect Disease control Fusarium oxysporum f. sp. fragaria (Fof) Strawberry Fusarium wilt (SFW) Trichoderma harzianum |
| url | http://www.sciencedirect.com/science/article/pii/S2468014123001784 |
| work_keys_str_mv | AT haoyang 5aminolevulinicacidagainststrawberryfusariumwiltbidirectionalregulationofbiocontrolagentsandpathogens AT jiantingzhang 5aminolevulinicacidagainststrawberryfusariumwiltbidirectionalregulationofbiocontrolagentsandpathogens AT haiwenzhang 5aminolevulinicacidagainststrawberryfusariumwiltbidirectionalregulationofbiocontrolagentsandpathogens AT rongxiangcao 5aminolevulinicacidagainststrawberryfusariumwiltbidirectionalregulationofbiocontrolagentsandpathogens AT donglantang 5aminolevulinicacidagainststrawberryfusariumwiltbidirectionalregulationofbiocontrolagentsandpathogens AT liangjuwang 5aminolevulinicacidagainststrawberryfusariumwiltbidirectionalregulationofbiocontrolagentsandpathogens |