Photobiomodulation Therapy Decreases Oxidative Stress in the Lung Tissue after Formaldehyde Exposure: Role of Oxidant/Antioxidant Enzymes
Formaldehyde is ubiquitous pollutant that induces oxidative stress in the lung. Several lung diseases have been associated with oxidative stress and their control is necessary. Photobiomodulation therapy (PBMT) has been highlighted as a promissory treatment, but its mechanisms need to be better inve...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/9303126 |
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| author | Rodrigo Silva Macedo Mayara Peres Leal Tarcio Teodoro Braga Éric Diego Barioni Stephanie de Oliveira Duro Anna Carolina Ratto Tempestini Horliana Niels Olsen Saraiva Câmara Tânia Marcourakis Sandra Helena Poliselli Farsky Adriana Lino-dos-Santos-Franco |
| author_facet | Rodrigo Silva Macedo Mayara Peres Leal Tarcio Teodoro Braga Éric Diego Barioni Stephanie de Oliveira Duro Anna Carolina Ratto Tempestini Horliana Niels Olsen Saraiva Câmara Tânia Marcourakis Sandra Helena Poliselli Farsky Adriana Lino-dos-Santos-Franco |
| author_sort | Rodrigo Silva Macedo |
| collection | DOAJ |
| description | Formaldehyde is ubiquitous pollutant that induces oxidative stress in the lung. Several lung diseases have been associated with oxidative stress and their control is necessary. Photobiomodulation therapy (PBMT) has been highlighted as a promissory treatment, but its mechanisms need to be better investigated. Our objective was to evaluate the effects of PBMT on the oxidative stress generated by FA exposure. Male Wistar rats were submitted to FA exposure of 1% or vehicle (3 days) and treated or not with PBMT (1 and 5 h after each FA exposure). Rats treated only with laser were used as control. Twenty-four hours after the last FA exposure, we analyzed the effects of PBMT on the generation of nitrites and hydrogen peroxide, oxidative burst, glutathione reductase, peroxidase, S-transferase enzyme activities, the gene expression of nitric oxide, cyclooxygenase, superoxide dismutase, the catalase enzyme, and heme oxygenase-1. PBMT reduced the generation of nitrites and hydrogen peroxide and increased oxidative burst in the lung cells. A decreased level of oxidant enzymes was observed which were concomitantly related to an increased level of antioxidants. This study provides new information about the antioxidant mechanisms of PBMT in the lung and might constitute an important tool for lung disease treatment. |
| format | Article |
| id | doaj-art-f46e05a8ef3e4e2fbd311d76e92613f1 |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-f46e05a8ef3e4e2fbd311d76e92613f12025-08-20T02:22:10ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/93031269303126Photobiomodulation Therapy Decreases Oxidative Stress in the Lung Tissue after Formaldehyde Exposure: Role of Oxidant/Antioxidant EnzymesRodrigo Silva Macedo0Mayara Peres Leal1Tarcio Teodoro Braga2Éric Diego Barioni3Stephanie de Oliveira Duro4Anna Carolina Ratto Tempestini Horliana5Niels Olsen Saraiva Câmara6Tânia Marcourakis7Sandra Helena Poliselli Farsky8Adriana Lino-dos-Santos-Franco9Postgraduate Program in Biophotonics Applied to Health Sciences, University Nove de Julho (UNINOVE), Rua Vergueiro, 235/249 Liberdade, 01504-001 São Paulo, SP, BrazilPostgraduate Program in Biophotonics Applied to Health Sciences, University Nove de Julho (UNINOVE), Rua Vergueiro, 235/249 Liberdade, 01504-001 São Paulo, SP, BrazilDepartment of Immunology, University of São Paulo, São Paulo, BrazilDepartment of Clinical and Toxicological Analyses, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, BrazilDepartment of Clinical and Toxicological Analyses, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, BrazilPostgraduate Program in Biophotonics Applied to Health Sciences, University Nove de Julho (UNINOVE), Rua Vergueiro, 235/249 Liberdade, 01504-001 São Paulo, SP, BrazilDepartment of Immunology, University of São Paulo, São Paulo, BrazilDepartment of Clinical and Toxicological Analyses, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, BrazilDepartment of Clinical and Toxicological Analyses, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, BrazilPostgraduate Program in Biophotonics Applied to Health Sciences, University Nove de Julho (UNINOVE), Rua Vergueiro, 235/249 Liberdade, 01504-001 São Paulo, SP, BrazilFormaldehyde is ubiquitous pollutant that induces oxidative stress in the lung. Several lung diseases have been associated with oxidative stress and their control is necessary. Photobiomodulation therapy (PBMT) has been highlighted as a promissory treatment, but its mechanisms need to be better investigated. Our objective was to evaluate the effects of PBMT on the oxidative stress generated by FA exposure. Male Wistar rats were submitted to FA exposure of 1% or vehicle (3 days) and treated or not with PBMT (1 and 5 h after each FA exposure). Rats treated only with laser were used as control. Twenty-four hours after the last FA exposure, we analyzed the effects of PBMT on the generation of nitrites and hydrogen peroxide, oxidative burst, glutathione reductase, peroxidase, S-transferase enzyme activities, the gene expression of nitric oxide, cyclooxygenase, superoxide dismutase, the catalase enzyme, and heme oxygenase-1. PBMT reduced the generation of nitrites and hydrogen peroxide and increased oxidative burst in the lung cells. A decreased level of oxidant enzymes was observed which were concomitantly related to an increased level of antioxidants. This study provides new information about the antioxidant mechanisms of PBMT in the lung and might constitute an important tool for lung disease treatment.http://dx.doi.org/10.1155/2016/9303126 |
| spellingShingle | Rodrigo Silva Macedo Mayara Peres Leal Tarcio Teodoro Braga Éric Diego Barioni Stephanie de Oliveira Duro Anna Carolina Ratto Tempestini Horliana Niels Olsen Saraiva Câmara Tânia Marcourakis Sandra Helena Poliselli Farsky Adriana Lino-dos-Santos-Franco Photobiomodulation Therapy Decreases Oxidative Stress in the Lung Tissue after Formaldehyde Exposure: Role of Oxidant/Antioxidant Enzymes Mediators of Inflammation |
| title | Photobiomodulation Therapy Decreases Oxidative Stress in the Lung Tissue after Formaldehyde Exposure: Role of Oxidant/Antioxidant Enzymes |
| title_full | Photobiomodulation Therapy Decreases Oxidative Stress in the Lung Tissue after Formaldehyde Exposure: Role of Oxidant/Antioxidant Enzymes |
| title_fullStr | Photobiomodulation Therapy Decreases Oxidative Stress in the Lung Tissue after Formaldehyde Exposure: Role of Oxidant/Antioxidant Enzymes |
| title_full_unstemmed | Photobiomodulation Therapy Decreases Oxidative Stress in the Lung Tissue after Formaldehyde Exposure: Role of Oxidant/Antioxidant Enzymes |
| title_short | Photobiomodulation Therapy Decreases Oxidative Stress in the Lung Tissue after Formaldehyde Exposure: Role of Oxidant/Antioxidant Enzymes |
| title_sort | photobiomodulation therapy decreases oxidative stress in the lung tissue after formaldehyde exposure role of oxidant antioxidant enzymes |
| url | http://dx.doi.org/10.1155/2016/9303126 |
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