Detection of multidrug-resistant methicillin-resistant Staphylococcus aureus from healthy black Bengal goat in Bangladesh
Introduction: The emergence of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is a growing public health concern. The objective of this study was to determine the prevalence and multi-drug resistant (MDR) profiles of MRSA in goats in Bangladesh. Methodology: A total of...
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The Journal of Infection in Developing Countries
2024-12-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/19701 |
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| author | Sarbani Biswas Md. Ariful Islam Jahidul Islam Mst. Minara Khatun Md. Zaminur Rahman |
| author_facet | Sarbani Biswas Md. Ariful Islam Jahidul Islam Mst. Minara Khatun Md. Zaminur Rahman |
| author_sort | Sarbani Biswas |
| collection | DOAJ |
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Introduction: The emergence of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is a growing public health concern. The objective of this study was to determine the prevalence and multi-drug resistant (MDR) profiles of MRSA in goats in Bangladesh.
Methodology: A total of 150 samples from goats comprised of rectal swab (n = 50), nasal swab (n = 50), and milk (n = 50) were collected. Isolation of S. aureus from samples was conducted onto mannitol salt agar (MSA). Identification of S. aureus was performed by cultural characteristics, Gram staining, biochemical tests (catalase, coagulase, indole, methyl red, and Voges-Proskaur), and nuc gene-specific PCR assay. The MRSA was identified by cefoxitin disc diffusion test and mecA gene-specific PCR assay. The MDR profiles of MRSA were performed against ampicillin, amoxicillin, gentamicin, cefoxitin, vancomycin, azithromycin, cefotaxime, ciprofloxacin and nalidixic acid by disc diffusion method.
Results: The overall prevalence of S. aureus was 35.3% and MRSA was 7.3%. The prevalence of MRSA was 12% in rectal swabs, 8% in nasal swabs, and 2% in milk. The highest resistance of MRSA was against ampicillin (91%) followed by azithromycin (55%), amoxycillin (36%), nalidixic acid (27%), ciprofloxacin (18%) and cefotaxime (9%). Most MRSA isolates (90.9%) exhibited resistance to at least three classes of antibiotics and were MDR.
Conclusions: This study shows that goats may harbor MDR-MRSA, posing a risk to public health.
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| format | Article |
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| issn | 1972-2680 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-f46ba82a6da647cbae1375b8a0e5e5da2025-08-20T02:27:22ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802024-12-01181210.3855/jidc.19701Detection of multidrug-resistant methicillin-resistant Staphylococcus aureus from healthy black Bengal goat in BangladeshSarbani Biswas0Md. Ariful Islam1Jahidul Islam2Mst. Minara Khatun3Md. Zaminur Rahman4Department of Microbiology & Hygiene, Bangladesh Agricultural University, Mymensingh-2202, BangladeshDepartment of Microbiology & Hygiene, Bangladesh Agricultural University, Mymensingh-2202, BangladeshDepartment of Microbiology & Hygiene, Bangladesh Agricultural University, Mymensingh-2202, BangladeshDepartment of Microbiology & Hygiene, Bangladesh Agricultural University, Mymensingh-2202, BangladeshDepartment of Microbiology & Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh Introduction: The emergence of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is a growing public health concern. The objective of this study was to determine the prevalence and multi-drug resistant (MDR) profiles of MRSA in goats in Bangladesh. Methodology: A total of 150 samples from goats comprised of rectal swab (n = 50), nasal swab (n = 50), and milk (n = 50) were collected. Isolation of S. aureus from samples was conducted onto mannitol salt agar (MSA). Identification of S. aureus was performed by cultural characteristics, Gram staining, biochemical tests (catalase, coagulase, indole, methyl red, and Voges-Proskaur), and nuc gene-specific PCR assay. The MRSA was identified by cefoxitin disc diffusion test and mecA gene-specific PCR assay. The MDR profiles of MRSA were performed against ampicillin, amoxicillin, gentamicin, cefoxitin, vancomycin, azithromycin, cefotaxime, ciprofloxacin and nalidixic acid by disc diffusion method. Results: The overall prevalence of S. aureus was 35.3% and MRSA was 7.3%. The prevalence of MRSA was 12% in rectal swabs, 8% in nasal swabs, and 2% in milk. The highest resistance of MRSA was against ampicillin (91%) followed by azithromycin (55%), amoxycillin (36%), nalidixic acid (27%), ciprofloxacin (18%) and cefotaxime (9%). Most MRSA isolates (90.9%) exhibited resistance to at least three classes of antibiotics and were MDR. Conclusions: This study shows that goats may harbor MDR-MRSA, posing a risk to public health. https://jidc.org/index.php/journal/article/view/19701Methicillin-resistant Staphylococcus aureusMRSAhealthy goatpublic health |
| spellingShingle | Sarbani Biswas Md. Ariful Islam Jahidul Islam Mst. Minara Khatun Md. Zaminur Rahman Detection of multidrug-resistant methicillin-resistant Staphylococcus aureus from healthy black Bengal goat in Bangladesh Journal of Infection in Developing Countries Methicillin-resistant Staphylococcus aureus MRSA healthy goat public health |
| title | Detection of multidrug-resistant methicillin-resistant Staphylococcus aureus from healthy black Bengal goat in Bangladesh |
| title_full | Detection of multidrug-resistant methicillin-resistant Staphylococcus aureus from healthy black Bengal goat in Bangladesh |
| title_fullStr | Detection of multidrug-resistant methicillin-resistant Staphylococcus aureus from healthy black Bengal goat in Bangladesh |
| title_full_unstemmed | Detection of multidrug-resistant methicillin-resistant Staphylococcus aureus from healthy black Bengal goat in Bangladesh |
| title_short | Detection of multidrug-resistant methicillin-resistant Staphylococcus aureus from healthy black Bengal goat in Bangladesh |
| title_sort | detection of multidrug resistant methicillin resistant staphylococcus aureus from healthy black bengal goat in bangladesh |
| topic | Methicillin-resistant Staphylococcus aureus MRSA healthy goat public health |
| url | https://jidc.org/index.php/journal/article/view/19701 |
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