The type I-E CRISPR-Cas system influences the acquisition of blaKPC-IncF plasmid in Klebsiella pneumonia
Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-KP) have disseminated worldwide and emerged as major threats to public health. Of epidemiological significance, the international pandemic of KPC-KP is primarily associated with CG258 isolates and blaKPC-IncF plasmids. CRISPR-Cas...
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Taylor & Francis Group
2020-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2020.1763209 |
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| author | Ying Zhou Yu Tang Pan Fu Dongxing Tian Lianhua Yu Yunkun Huang Gang Li Meng Li Yong Wang Zehua Yang Xiaogang Xu Zhe Yin Dongsheng Zhou Laurent Poirel Xiaofei Jiang |
| author_facet | Ying Zhou Yu Tang Pan Fu Dongxing Tian Lianhua Yu Yunkun Huang Gang Li Meng Li Yong Wang Zehua Yang Xiaogang Xu Zhe Yin Dongsheng Zhou Laurent Poirel Xiaofei Jiang |
| author_sort | Ying Zhou |
| collection | DOAJ |
| description | Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-KP) have disseminated worldwide and emerged as major threats to public health. Of epidemiological significance, the international pandemic of KPC-KP is primarily associated with CG258 isolates and blaKPC-IncF plasmids. CRISPR-Cas system is an adaptive immune system that can hinder gene expansion driven by horizontal gene transfer. Because of blaKPC-IncF plasmids are favored by CG258 K. pneumoniae, it was of interest to examine the co-distribution of CRISPR and blaKPC-IncF plasmids in such isolates. We collected 459 clinical K. pneumoniae isolates in China and collected 203 global whole-genome sequences in GenBank to determine the prevalence of CRISPR-Cas systems. We observed that CRISPR-Cas system was significantly scarce in the CG258 lineage and blaKPC-positive isolates. Furthermore, the results of conjugation and plasmid stability assay fully demonstrated the CRIPSR-Cas system in K. pneumoniae could effectively hindered blaKPC-IncF plasmids invasion and existence. Notably, most blaKPC-IncF plasmids were also proved to be good targets of CRISPR owing to carry matched and functional protospacers and PAMs. Overall, our work suggests that type I-E CRISPR-Cas systems could impact the spread of blaKPC in K. pneumoniae populations, and the scarcity of CRISPR-Cas system was one of potential factors leading to the propagation of blaKPC-IncF plasmids in CG258 K. pneumoniae. |
| format | Article |
| id | doaj-art-f45b025284fe40abbcb28f393e64ccff |
| institution | OA Journals |
| issn | 2222-1751 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-f45b025284fe40abbcb28f393e64ccff2025-08-20T02:12:15ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-01911011102210.1080/22221751.2020.1763209The type I-E CRISPR-Cas system influences the acquisition of blaKPC-IncF plasmid in Klebsiella pneumoniaYing Zhou0Yu Tang1Pan Fu2Dongxing Tian3Lianhua Yu4Yunkun Huang5Gang Li6Meng Li7Yong Wang8Zehua Yang9Xiaogang Xu10Zhe Yin11Dongsheng Zhou12Laurent Poirel13Xiaofei Jiang14Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, People’s Republic of ChinaDepartment of Laboratory Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaDepartment of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, People’s Republic of ChinaDepartment of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, People’s Republic of ChinaDepartment of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, People’s Republic of ChinaDepartment of Laboratory Medicine Kunming Yan’an Hospital, Kunming, People’s Republic of ChinaDepartment of Laboratory Medicine, Jinshan Hospital, Shanghai Medical College, Fudan University, Shanghai, People’s Republic of ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University, Nanning, People’s Republic of ChinaDepartment of Clinical Laboratory, Shandong Provincial Hospital affiliated to Shandong University, Jinan, People’s Republic of ChinaDepartment of Laboratory Medicine, Sixth Hospital of Shanxi Medical University, Taiyuan, People’s Republic of ChinaInstitute of Antibiotics, Huashan Hospital, Fudan University, and Key Laboratory of Clinical Pharmacology of Antibiotics, National Health and Family Planning Commission, Shanghai, People’s Republic of ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, People’s Republic of ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, People’s Republic of ChinaEmerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Fribourg, SwitzerlandDepartment of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, People’s Republic of ChinaKlebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-KP) have disseminated worldwide and emerged as major threats to public health. Of epidemiological significance, the international pandemic of KPC-KP is primarily associated with CG258 isolates and blaKPC-IncF plasmids. CRISPR-Cas system is an adaptive immune system that can hinder gene expansion driven by horizontal gene transfer. Because of blaKPC-IncF plasmids are favored by CG258 K. pneumoniae, it was of interest to examine the co-distribution of CRISPR and blaKPC-IncF plasmids in such isolates. We collected 459 clinical K. pneumoniae isolates in China and collected 203 global whole-genome sequences in GenBank to determine the prevalence of CRISPR-Cas systems. We observed that CRISPR-Cas system was significantly scarce in the CG258 lineage and blaKPC-positive isolates. Furthermore, the results of conjugation and plasmid stability assay fully demonstrated the CRIPSR-Cas system in K. pneumoniae could effectively hindered blaKPC-IncF plasmids invasion and existence. Notably, most blaKPC-IncF plasmids were also proved to be good targets of CRISPR owing to carry matched and functional protospacers and PAMs. Overall, our work suggests that type I-E CRISPR-Cas systems could impact the spread of blaKPC in K. pneumoniae populations, and the scarcity of CRISPR-Cas system was one of potential factors leading to the propagation of blaKPC-IncF plasmids in CG258 K. pneumoniae.https://www.tandfonline.com/doi/10.1080/22221751.2020.1763209CRISPR-CasKlebsiella pneumoniae clonal complex 258carbapenem resistanceplasmidshorizontal gene transfer |
| spellingShingle | Ying Zhou Yu Tang Pan Fu Dongxing Tian Lianhua Yu Yunkun Huang Gang Li Meng Li Yong Wang Zehua Yang Xiaogang Xu Zhe Yin Dongsheng Zhou Laurent Poirel Xiaofei Jiang The type I-E CRISPR-Cas system influences the acquisition of blaKPC-IncF plasmid in Klebsiella pneumonia Emerging Microbes and Infections CRISPR-Cas Klebsiella pneumoniae clonal complex 258 carbapenem resistance plasmids horizontal gene transfer |
| title | The type I-E CRISPR-Cas system influences the acquisition of blaKPC-IncF plasmid in Klebsiella pneumonia |
| title_full | The type I-E CRISPR-Cas system influences the acquisition of blaKPC-IncF plasmid in Klebsiella pneumonia |
| title_fullStr | The type I-E CRISPR-Cas system influences the acquisition of blaKPC-IncF plasmid in Klebsiella pneumonia |
| title_full_unstemmed | The type I-E CRISPR-Cas system influences the acquisition of blaKPC-IncF plasmid in Klebsiella pneumonia |
| title_short | The type I-E CRISPR-Cas system influences the acquisition of blaKPC-IncF plasmid in Klebsiella pneumonia |
| title_sort | type i e crispr cas system influences the acquisition of blakpc incf plasmid in klebsiella pneumonia |
| topic | CRISPR-Cas Klebsiella pneumoniae clonal complex 258 carbapenem resistance plasmids horizontal gene transfer |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2020.1763209 |
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