Genomic insights into a clinical Salmonella Typhimurium isolate carrying plasmid-mediated blaNDM-5
Objective: Highly carbapenem-resistant Salmonella has emerged worldwide in recent years and is largely associated with the multiform transmission of resistance genes, which poses a huge challenge in clinical practice. Our study delves into the resistance mechanisms and epidemiology of blaNDM-carryin...
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Elsevier
2025-01-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716524004508 |
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| author | Fan Huang Genglin Guo Lu Feng Tongbo Cai Xu Huang |
| author_facet | Fan Huang Genglin Guo Lu Feng Tongbo Cai Xu Huang |
| author_sort | Fan Huang |
| collection | DOAJ |
| description | Objective: Highly carbapenem-resistant Salmonella has emerged worldwide in recent years and is largely associated with the multiform transmission of resistance genes, which poses a huge challenge in clinical practice. Our study delves into the resistance mechanisms and epidemiology of blaNDM-carrying plasmids. Methods: Whole-genome sequencing was utilised to analyse the molecular characteristics and antimicrobial resistance mechanisms of Salmonella isolates recovered from the faeces of a paediatric patient at the Children's Hospital of Nanjing Medical University. Moreover, we conducted an epidemiologic analysis and focused on studying the mechanisms of plasmid-mediated blaNDM transmission, incorporating genomes deposited in the NCBI Pathogen Detection database. Results: The clinical isolate 23S9 belonged to serovar Typhimurium, antigenic profile 4:i:-, ST34, and carried pNDM_23S9 harbouring several antimicrobial resistance genes, including aac(6′)-Ib-cr6, OXA-1, catB3, arr-3, qacEdeltal and blaNDM. Comparative analysis revealed that blaNDM-5 can exist in different plasmids of different isolates, proving its transmission through plasmids. Furthermore, blaNDM-carrying isolates are mostly resistant to beta-lactams, aminoglycosides, sulphonamide, macrolides, and trimethoprim. Conclusions: These findings provided thorough and intuitive insights into the intercontinental spread of blaNDM-carrying Salmonella. Ongoing surveillance is essential for effectively monitoring the worldwide dissemination of this high-risk carbapenem-resistant Salmonella. |
| format | Article |
| id | doaj-art-f459b45f08fb4083ad6e6ecfef6ad3b6 |
| institution | Kabale University |
| issn | 2213-7165 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-f459b45f08fb4083ad6e6ecfef6ad3b62025-02-08T05:00:25ZengElsevierJournal of Global Antimicrobial Resistance2213-71652025-01-01409095Genomic insights into a clinical Salmonella Typhimurium isolate carrying plasmid-mediated blaNDM-5Fan Huang0Genglin Guo1Lu Feng2Tongbo Cai3Xu Huang4Department of Clinical Laboratory, Children's Hospital of Nanjing Medical University, Nanjing, ChinaShandong Institute of Sericulture, Jiaodong Innovation Center, Shandong Academy of Agricultural Sciences, Yantai, ChinaSchool of Mechanical Engineering, Tongji University, Shanghai, ChinaCollege of Computer and Information Engineering, Henan Normal University, Xinxiang, ChinaDepartment of Clinical Laboratory, Children's Hospital of Nanjing Medical University, Nanjing, China; Corresponding author at: Department of Clinical Laboratory, Children’s Hospital of Nanjing Medical University, Nanjing 210000, China.Objective: Highly carbapenem-resistant Salmonella has emerged worldwide in recent years and is largely associated with the multiform transmission of resistance genes, which poses a huge challenge in clinical practice. Our study delves into the resistance mechanisms and epidemiology of blaNDM-carrying plasmids. Methods: Whole-genome sequencing was utilised to analyse the molecular characteristics and antimicrobial resistance mechanisms of Salmonella isolates recovered from the faeces of a paediatric patient at the Children's Hospital of Nanjing Medical University. Moreover, we conducted an epidemiologic analysis and focused on studying the mechanisms of plasmid-mediated blaNDM transmission, incorporating genomes deposited in the NCBI Pathogen Detection database. Results: The clinical isolate 23S9 belonged to serovar Typhimurium, antigenic profile 4:i:-, ST34, and carried pNDM_23S9 harbouring several antimicrobial resistance genes, including aac(6′)-Ib-cr6, OXA-1, catB3, arr-3, qacEdeltal and blaNDM. Comparative analysis revealed that blaNDM-5 can exist in different plasmids of different isolates, proving its transmission through plasmids. Furthermore, blaNDM-carrying isolates are mostly resistant to beta-lactams, aminoglycosides, sulphonamide, macrolides, and trimethoprim. Conclusions: These findings provided thorough and intuitive insights into the intercontinental spread of blaNDM-carrying Salmonella. Ongoing surveillance is essential for effectively monitoring the worldwide dissemination of this high-risk carbapenem-resistant Salmonella.http://www.sciencedirect.com/science/article/pii/S2213716524004508Salmonella TyphimuriumBlaNDM-5Whole-genome sequencing |
| spellingShingle | Fan Huang Genglin Guo Lu Feng Tongbo Cai Xu Huang Genomic insights into a clinical Salmonella Typhimurium isolate carrying plasmid-mediated blaNDM-5 Journal of Global Antimicrobial Resistance Salmonella Typhimurium BlaNDM-5 Whole-genome sequencing |
| title | Genomic insights into a clinical Salmonella Typhimurium isolate carrying plasmid-mediated blaNDM-5 |
| title_full | Genomic insights into a clinical Salmonella Typhimurium isolate carrying plasmid-mediated blaNDM-5 |
| title_fullStr | Genomic insights into a clinical Salmonella Typhimurium isolate carrying plasmid-mediated blaNDM-5 |
| title_full_unstemmed | Genomic insights into a clinical Salmonella Typhimurium isolate carrying plasmid-mediated blaNDM-5 |
| title_short | Genomic insights into a clinical Salmonella Typhimurium isolate carrying plasmid-mediated blaNDM-5 |
| title_sort | genomic insights into a clinical salmonella typhimurium isolate carrying plasmid mediated blandm 5 |
| topic | Salmonella Typhimurium BlaNDM-5 Whole-genome sequencing |
| url | http://www.sciencedirect.com/science/article/pii/S2213716524004508 |
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