Identification and Characterization of <i>Fusarium incarnatum</i> Causing Leaf Spot and Fruit Rot on Luffa in China

In 2022, an outbreak of fungal rot disease affected luffa crops in Shanghai and Zhejiang Province. Infected plants exhibited symptoms including yellowing, chlorosis, wilting, and water-soaked occurred on leaves and fruits. Dark brown, concave lesions developed, often accompanied by white or pale pin...

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Bibliographic Details
Main Authors: Xia Chen, Hao Liu, Lanlan Dong, Junrui Shi, Zhonghua Ma, Leiyan Yan, Yanni Yin
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/14/6/845
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Summary:In 2022, an outbreak of fungal rot disease affected luffa crops in Shanghai and Zhejiang Province. Infected plants exhibited symptoms including yellowing, chlorosis, wilting, and water-soaked occurred on leaves and fruits. Dark brown, concave lesions developed, often accompanied by white or pale pink mold under moist conditions. Fourteen pathogen strains, morphologically resembling <i>Fusarium</i> species, were isolated. Molecular analysis confirmed <i>Fusarium incarnatum</i> as the causative agent. Pathogenicity tests on luffa plants fulfilled Koch’s postulates, with inoculated plants displaying the same symptoms. Re-isolation of the fungus from the inoculated plants confirmed its role in the disease. To our knowledge, this is the first report of <i>F. incarnatum</i> causing leaf spot and fruit rot on luffa in China. Moreover, the soil bacterial strain <i>Bacillus velezensis</i> BV171 displayed strong inhibition of <i>F. incarnatum</i> mycelia growth and promoted the growth of sponge gourd plants. These findings lay the foundation for the development of diagnostic tools, disease management strategies, and the breeding of resistant luffa varieties.
ISSN:2223-7747