Genetic Diversity Analysis of Mulberry Silkworm (Bombyx Mori L.) Hybrids and Breeds in Azerbaijan Using RAPD Markers
In order to study the genetic diversity of silkworms (Bombyx mori L.) breeding in Azerbaijan, the following breeds and hybrids were analyzed: SHZEM 4 x GE 143, Chingiz x Yagub, Yagub x Chingiz, GE 143, Yagub, SHZEM 4 belted, Chingiz, GE 143 x SHZEM 4 using RAPD molecular markers. For this purpose, s...
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EDP Sciences
2025-01-01
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| Series: | BIO Web of Conferences |
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| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2025/02/bioconf_mblc2024_01009.pdf |
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| author | Mammadov Ayaz Aghayeva Saltanat Bakırov Guduret Gulubeyova Mahpuba Ismayılova Gular |
| author_facet | Mammadov Ayaz Aghayeva Saltanat Bakırov Guduret Gulubeyova Mahpuba Ismayılova Gular |
| author_sort | Mammadov Ayaz |
| collection | DOAJ |
| description | In order to study the genetic diversity of silkworms (Bombyx mori L.) breeding in Azerbaijan, the following breeds and hybrids were analyzed: SHZEM 4 x GE 143, Chingiz x Yagub, Yagub x Chingiz, GE 143, Yagub, SHZEM 4 belted, Chingiz, GE 143 x SHZEM 4 using RAPD molecular markers. For this purpose, silkworm larvae were taken for DNA extraction and nuclear genome DNA was isolated from various parts of the silkworm (silk gland, epidermis, etc.). Four RAPD primers (BGN 04, BGL 02, BGY 06, BGA 02) were used in the study, resulting in 200-1200 bp 34 dots representing fragments. The average number of amplicons for each primer was 6-11, and 82-100% of the amplicons were polymorphic. The study found that RAPD markers do not fully reflect the similarity between the local breed and hybrids. |
| format | Article |
| id | doaj-art-f4478bc5bb664e18999652585f9c763f |
| institution | Kabale University |
| issn | 2117-4458 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj-art-f4478bc5bb664e18999652585f9c763f2025-02-05T10:42:41ZengEDP SciencesBIO Web of Conferences2117-44582025-01-011510100910.1051/bioconf/202515101009bioconf_mblc2024_01009Genetic Diversity Analysis of Mulberry Silkworm (Bombyx Mori L.) Hybrids and Breeds in Azerbaijan Using RAPD MarkersMammadov Ayaz0Aghayeva Saltanat1Bakırov Guduret2Gulubeyova Mahpuba3Ismayılova Gular4Department of Natural Sciences, Western Caspian UniversityDepartment of Natural Sciences, Western Caspian UniversityDepartment of Silkworms Breeding, Sheki Regional Scientific Center of ANASDepartment of Breeding and Nutrition of Agricultural Animals of the Azerbaijan State Agrarian UniversityGenetic Resources Institute of the Ministry of Science and EducationIn order to study the genetic diversity of silkworms (Bombyx mori L.) breeding in Azerbaijan, the following breeds and hybrids were analyzed: SHZEM 4 x GE 143, Chingiz x Yagub, Yagub x Chingiz, GE 143, Yagub, SHZEM 4 belted, Chingiz, GE 143 x SHZEM 4 using RAPD molecular markers. For this purpose, silkworm larvae were taken for DNA extraction and nuclear genome DNA was isolated from various parts of the silkworm (silk gland, epidermis, etc.). Four RAPD primers (BGN 04, BGL 02, BGY 06, BGA 02) were used in the study, resulting in 200-1200 bp 34 dots representing fragments. The average number of amplicons for each primer was 6-11, and 82-100% of the amplicons were polymorphic. The study found that RAPD markers do not fully reflect the similarity between the local breed and hybrids.https://www.bio-conferences.org/articles/bioconf/pdf/2025/02/bioconf_mblc2024_01009.pdfsilkwormbreedshybridshomozygotehereditary |
| spellingShingle | Mammadov Ayaz Aghayeva Saltanat Bakırov Guduret Gulubeyova Mahpuba Ismayılova Gular Genetic Diversity Analysis of Mulberry Silkworm (Bombyx Mori L.) Hybrids and Breeds in Azerbaijan Using RAPD Markers BIO Web of Conferences silkworm breeds hybrids homozygote hereditary |
| title | Genetic Diversity Analysis of Mulberry Silkworm (Bombyx Mori L.) Hybrids and Breeds in Azerbaijan Using RAPD Markers |
| title_full | Genetic Diversity Analysis of Mulberry Silkworm (Bombyx Mori L.) Hybrids and Breeds in Azerbaijan Using RAPD Markers |
| title_fullStr | Genetic Diversity Analysis of Mulberry Silkworm (Bombyx Mori L.) Hybrids and Breeds in Azerbaijan Using RAPD Markers |
| title_full_unstemmed | Genetic Diversity Analysis of Mulberry Silkworm (Bombyx Mori L.) Hybrids and Breeds in Azerbaijan Using RAPD Markers |
| title_short | Genetic Diversity Analysis of Mulberry Silkworm (Bombyx Mori L.) Hybrids and Breeds in Azerbaijan Using RAPD Markers |
| title_sort | genetic diversity analysis of mulberry silkworm bombyx mori l hybrids and breeds in azerbaijan using rapd markers |
| topic | silkworm breeds hybrids homozygote hereditary |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2025/02/bioconf_mblc2024_01009.pdf |
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