Genetic Diversity Analysis of Mulberry Silkworm (Bombyx Mori L.) Hybrids and Breeds in Azerbaijan Using RAPD Markers
In order to study the genetic diversity of silkworms (Bombyx mori L.) breeding in Azerbaijan, the following breeds and hybrids were analyzed: SHZEM 4 x GE 143, Chingiz x Yagub, Yagub x Chingiz, GE 143, Yagub, SHZEM 4 belted, Chingiz, GE 143 x SHZEM 4 using RAPD molecular markers. For this purpose, s...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2025-01-01
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| Series: | BIO Web of Conferences |
| Subjects: | |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2025/02/bioconf_mblc2024_01009.pdf |
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| Summary: | In order to study the genetic diversity of silkworms (Bombyx mori L.) breeding in Azerbaijan, the following breeds and hybrids were analyzed: SHZEM 4 x GE 143, Chingiz x Yagub, Yagub x Chingiz, GE 143, Yagub, SHZEM 4 belted, Chingiz, GE 143 x SHZEM 4 using RAPD molecular markers. For this purpose, silkworm larvae were taken for DNA extraction and nuclear genome DNA was isolated from various parts of the silkworm (silk gland, epidermis, etc.). Four RAPD primers (BGN 04, BGL 02, BGY 06, BGA 02) were used in the study, resulting in 200-1200 bp 34 dots representing fragments. The average number of amplicons for each primer was 6-11, and 82-100% of the amplicons were polymorphic. The study found that RAPD markers do not fully reflect the similarity between the local breed and hybrids. |
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| ISSN: | 2117-4458 |