Detection of O25b-ST131 clone in extended spectrum beta-lactamase-producing E. coli from urinary tract infections in Mexico
Introduction: Escherichia coli has emerged as an important pathogen in urinary tract infections (UTIs) due to the rapid acquisition of antibiotic resistance genes. This enhances the ability of E. coli to colonize and creates therapeutic challenges within the healthcare system. This study aimed to i...
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The Journal of Infection in Developing Countries
2024-12-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/18854 |
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| author | Josefina Duran-Bedolla Juan Téllez-Sosa Ana María González-Villoria Humberto Barrios-Camacho |
| author_facet | Josefina Duran-Bedolla Juan Téllez-Sosa Ana María González-Villoria Humberto Barrios-Camacho |
| author_sort | Josefina Duran-Bedolla |
| collection | DOAJ |
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Introduction: Escherichia coli has emerged as an important pathogen in urinary tract infections (UTIs) due to the rapid acquisition of antibiotic resistance genes. This enhances the ability of E. coli to colonize and creates therapeutic challenges within the healthcare system. This study aimed to identify the extended spectrum beta-lactamase (ESBL) and O25b-ST131 pandemic clones in E. coli isolated from two hospitals in Mexico.
Methodology: Bacterial identification and antibiotic susceptibility tests were conducted using the VITEK 2 system. The ESBL and plasmid-mediated quinolone resistance (PMQR) genes were identified by polymerase chain reaction (PCR). E. coli genotyping was carried out by the phylogenetic group analysis and O25b-ST131 identification.
Results: A total of 103 unique E. coli clinical isolates were analyzed from a pool of 1,002 strains; 75% obtained from UTIs and vaginal secretions. Multi-resistant antibiotic profiles were observed. Notably, the presence of the aac(6`)lb-cr and qnr genes was associated with 100% ciprofloxacin resistance, when ESBL was present. Additionally, the B2 phylogenetic group was identified, with 23% of isolates belonging to the O25b-ST131 clone.
Conclusions: Our research revealed a 10% prevalence of ESBL, in contrast to global prevalence rates. The resistance profiles suggest that the effectiveness of these commonly used antibiotics in treating E. coli-associated UTIs or vaginal infections has decreased significantly. Excessive use of antimicrobial agents contributes to the regional variation. Our results underscore the importance of monitoring the molecular epidemiology, antibiotic resistance, and transmission dynamics of the O25b-ST131 E. coli clone.
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| format | Article |
| id | doaj-art-f447571356ee44dea4e7bfc009669b2f |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-f447571356ee44dea4e7bfc009669b2f2025-08-20T03:52:39ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802024-12-01181210.3855/jidc.18854Detection of O25b-ST131 clone in extended spectrum beta-lactamase-producing E. coli from urinary tract infections in MexicoJosefina Duran-Bedolla0Juan Téllez-Sosa1Ana María González-Villoria2Humberto Barrios-Camacho3Instituto Nacional de Salud Pública (INSP), Centro de Investigación Sobre Enfermedades Infecciosas (CISEI), Departamento de Diagnóstico Epidemiológico. Cuernavaca, Morelos, MéxicoInstituto Nacional de Salud Pública (INSP), Centro de Investigación Sobre Enfermedades Infecciosas (CISEI), Departamento de Diagnóstico Epidemiológico. Cuernavaca, Morelos, MéxicoDivisión de Estudios de Posgrado. Universidad de la Sierra Sur, Oaxaca, MéxicoInstituto Nacional de Salud Pública (INSP), Centro de Investigación Sobre Enfermedades Infecciosas (CISEI), Departamento de Diagnóstico Epidemiológico. Cuernavaca, Morelos, México Introduction: Escherichia coli has emerged as an important pathogen in urinary tract infections (UTIs) due to the rapid acquisition of antibiotic resistance genes. This enhances the ability of E. coli to colonize and creates therapeutic challenges within the healthcare system. This study aimed to identify the extended spectrum beta-lactamase (ESBL) and O25b-ST131 pandemic clones in E. coli isolated from two hospitals in Mexico. Methodology: Bacterial identification and antibiotic susceptibility tests were conducted using the VITEK 2 system. The ESBL and plasmid-mediated quinolone resistance (PMQR) genes were identified by polymerase chain reaction (PCR). E. coli genotyping was carried out by the phylogenetic group analysis and O25b-ST131 identification. Results: A total of 103 unique E. coli clinical isolates were analyzed from a pool of 1,002 strains; 75% obtained from UTIs and vaginal secretions. Multi-resistant antibiotic profiles were observed. Notably, the presence of the aac(6`)lb-cr and qnr genes was associated with 100% ciprofloxacin resistance, when ESBL was present. Additionally, the B2 phylogenetic group was identified, with 23% of isolates belonging to the O25b-ST131 clone. Conclusions: Our research revealed a 10% prevalence of ESBL, in contrast to global prevalence rates. The resistance profiles suggest that the effectiveness of these commonly used antibiotics in treating E. coli-associated UTIs or vaginal infections has decreased significantly. Excessive use of antimicrobial agents contributes to the regional variation. Our results underscore the importance of monitoring the molecular epidemiology, antibiotic resistance, and transmission dynamics of the O25b-ST131 E. coli clone. https://jidc.org/index.php/journal/article/view/18854UTIESBLEscherichia coliST131 |
| spellingShingle | Josefina Duran-Bedolla Juan Téllez-Sosa Ana María González-Villoria Humberto Barrios-Camacho Detection of O25b-ST131 clone in extended spectrum beta-lactamase-producing E. coli from urinary tract infections in Mexico Journal of Infection in Developing Countries UTI ESBL Escherichia coli ST131 |
| title | Detection of O25b-ST131 clone in extended spectrum beta-lactamase-producing E. coli from urinary tract infections in Mexico |
| title_full | Detection of O25b-ST131 clone in extended spectrum beta-lactamase-producing E. coli from urinary tract infections in Mexico |
| title_fullStr | Detection of O25b-ST131 clone in extended spectrum beta-lactamase-producing E. coli from urinary tract infections in Mexico |
| title_full_unstemmed | Detection of O25b-ST131 clone in extended spectrum beta-lactamase-producing E. coli from urinary tract infections in Mexico |
| title_short | Detection of O25b-ST131 clone in extended spectrum beta-lactamase-producing E. coli from urinary tract infections in Mexico |
| title_sort | detection of o25b st131 clone in extended spectrum beta lactamase producing e coli from urinary tract infections in mexico |
| topic | UTI ESBL Escherichia coli ST131 |
| url | https://jidc.org/index.php/journal/article/view/18854 |
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