Genome-Wide Identification and Characterization of bHLH Transcription Factors and Their Expression Profile in Potato (<i>Solanum tuberosum</i> L.)

The <i>bHLH</i> gene family, one of the most abundant transcription factor families in plants, plays crucial roles in stress resistance, growth, and development. To explore the characteristics of the potato <i>bHLH</i> gene family members, this study identified and analyzed a...

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Main Authors: Jiali Ye, Lingzhi Zhang, Shuaikang Liu, Yi Diao, Lina Yin, Xiping Deng, Shiwen Wang
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Agronomy
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Online Access:https://www.mdpi.com/2073-4395/15/5/1070
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Summary:The <i>bHLH</i> gene family, one of the most abundant transcription factor families in plants, plays crucial roles in stress resistance, growth, and development. To explore the characteristics of the potato <i>bHLH</i> gene family members, this study identified and analyzed a total of 134 <i>bHLH</i> genes. Using bioinformatics approaches, we examined their physicochemical properties, conserved structural domains, motifs, and cis-acting elements. Additionally, a phylogenetic analysis was conducted, comparing the bHLH proteins of potato with those of the model plant <i>Arabidopsis thaliana</i>. The study also investigated the expression patterns of <i>StbHLH</i> genes under different environmental conditions and growth stages. The potato <i>bHLH</i> gene family is enriched with various cis-acting elements associated with stress response and plant hormone signaling. The expression patterns of <i>StbHLH</i> genes varied significantly across different conditions, revealing their potential roles in stress resistance and developmental processes. For example, under drought and re-watering treatments, distinct expression patterns were observed, with specific genes showing upregulation or downregulation at different time points. <i>StbHLH025</i> regulates tissue development and stress response in potato. These findings not only reveal the diversity and complexity of the potato <i>bHLH</i> gene family but also provide valuable insights for future research into the functions of <i>StbHLH</i> genes, particularly their roles in potato stress resistance and developmental processes.
ISSN:2073-4395