Blockade of Piezo2 Pathway Attenuates Inflammatory and Neuropathic Pain in the Orofacial Area
Although previous studies suggest that Piezo2 regulates chronic pain in the orofacial area, few studies have reported the direct evidence of Piezo2’s involvement in inflammatory and neuropathic pain in the orofacial region. In this study, we used male Sprague Dawley rats to investigate the role of t...
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Pain Research and Management |
| Online Access: | http://dx.doi.org/10.1155/2024/9179928 |
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| author | Min-Jeong Jo Jo-Young Son Yu-Mi Kim Jin-Sook Ju Min-Kyoung Park Min-Kyung Lee Dong-Kuk Ahn |
| author_facet | Min-Jeong Jo Jo-Young Son Yu-Mi Kim Jin-Sook Ju Min-Kyoung Park Min-Kyung Lee Dong-Kuk Ahn |
| author_sort | Min-Jeong Jo |
| collection | DOAJ |
| description | Although previous studies suggest that Piezo2 regulates chronic pain in the orofacial area, few studies have reported the direct evidence of Piezo2’s involvement in inflammatory and neuropathic pain in the orofacial region. In this study, we used male Sprague Dawley rats to investigate the role of the Piezo2 pathway in the development of inflammatory and neuropathic pain. The present study used interleukin (IL)-1β–induced pronociception as an inflammatory pain model. Subcutaneous injection of IL-1β produced significant mechanical allodynia and thermal hyperalgesia. Subcutaneous injection of a Piezo2 inhibitor significantly blocked mechanical allodynia and thermal hyperalgesia induced by subcutaneously injected IL-1β. Furthermore, the present study also used a neuropathic pain model caused by the misplacement of a dental implant, leading to notable mechanical allodynia as a consequence of inferior alveolar nerve injury. Western blot analysis revealed increased levels of Piezo2 in the trigeminal ganglion and the trigeminal subnucleus caudalis after inferior alveolar nerve injury. Furthermore, subcutaneous and intracisternal injections of a Piezo2 inhibitor blocked neuropathic mechanical allodynia. These results suggest that the Piezo2 pathway plays a critical role in the development of inflammatory and neuropathic pain in the orofacial area. Therefore, blocking the Piezo2 pathway could be the foundation for developing new therapeutic strategies to treat orofacial pain conditions. |
| format | Article |
| id | doaj-art-f41362fedfe04dab923d9f6de0e44b4b |
| institution | Kabale University |
| issn | 1918-1523 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Pain Research and Management |
| spelling | doaj-art-f41362fedfe04dab923d9f6de0e44b4b2025-02-03T12:01:45ZengWileyPain Research and Management1918-15232024-01-01202410.1155/2024/9179928Blockade of Piezo2 Pathway Attenuates Inflammatory and Neuropathic Pain in the Orofacial AreaMin-Jeong Jo0Jo-Young Son1Yu-Mi Kim2Jin-Sook Ju3Min-Kyoung Park4Min-Kyung Lee5Dong-Kuk Ahn6Department of Oral PhysiologyDepartment of Oral PhysiologyDepartment of Oral PhysiologyDepartment of Oral PhysiologyDepartment of Dental HygieneDepartment of Dental HygieneDepartment of Oral PhysiologyAlthough previous studies suggest that Piezo2 regulates chronic pain in the orofacial area, few studies have reported the direct evidence of Piezo2’s involvement in inflammatory and neuropathic pain in the orofacial region. In this study, we used male Sprague Dawley rats to investigate the role of the Piezo2 pathway in the development of inflammatory and neuropathic pain. The present study used interleukin (IL)-1β–induced pronociception as an inflammatory pain model. Subcutaneous injection of IL-1β produced significant mechanical allodynia and thermal hyperalgesia. Subcutaneous injection of a Piezo2 inhibitor significantly blocked mechanical allodynia and thermal hyperalgesia induced by subcutaneously injected IL-1β. Furthermore, the present study also used a neuropathic pain model caused by the misplacement of a dental implant, leading to notable mechanical allodynia as a consequence of inferior alveolar nerve injury. Western blot analysis revealed increased levels of Piezo2 in the trigeminal ganglion and the trigeminal subnucleus caudalis after inferior alveolar nerve injury. Furthermore, subcutaneous and intracisternal injections of a Piezo2 inhibitor blocked neuropathic mechanical allodynia. These results suggest that the Piezo2 pathway plays a critical role in the development of inflammatory and neuropathic pain in the orofacial area. Therefore, blocking the Piezo2 pathway could be the foundation for developing new therapeutic strategies to treat orofacial pain conditions.http://dx.doi.org/10.1155/2024/9179928 |
| spellingShingle | Min-Jeong Jo Jo-Young Son Yu-Mi Kim Jin-Sook Ju Min-Kyoung Park Min-Kyung Lee Dong-Kuk Ahn Blockade of Piezo2 Pathway Attenuates Inflammatory and Neuropathic Pain in the Orofacial Area Pain Research and Management |
| title | Blockade of Piezo2 Pathway Attenuates Inflammatory and Neuropathic Pain in the Orofacial Area |
| title_full | Blockade of Piezo2 Pathway Attenuates Inflammatory and Neuropathic Pain in the Orofacial Area |
| title_fullStr | Blockade of Piezo2 Pathway Attenuates Inflammatory and Neuropathic Pain in the Orofacial Area |
| title_full_unstemmed | Blockade of Piezo2 Pathway Attenuates Inflammatory and Neuropathic Pain in the Orofacial Area |
| title_short | Blockade of Piezo2 Pathway Attenuates Inflammatory and Neuropathic Pain in the Orofacial Area |
| title_sort | blockade of piezo2 pathway attenuates inflammatory and neuropathic pain in the orofacial area |
| url | http://dx.doi.org/10.1155/2024/9179928 |
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