Oral Vaccination Based on DNA-Chitosan Nanoparticles against Schistosoma mansoni Infection
The development of a vaccine would be essential for the control of schistosomiasis, which is recognized as the most important human helminth infection in terms of morbidity and mortality. A new approach of oral vaccination with DNA-chitosan nanoparticles appears interesting because of their great st...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/938457 |
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| author | Carolina R. Oliveira Cíntia M. F. Rezende Marina R. Silva Olga M. Borges Ana P. Pêgo Alfredo M. Goes |
| author_facet | Carolina R. Oliveira Cíntia M. F. Rezende Marina R. Silva Olga M. Borges Ana P. Pêgo Alfredo M. Goes |
| author_sort | Carolina R. Oliveira |
| collection | DOAJ |
| description | The development of a vaccine would be essential for the control of schistosomiasis, which is recognized as the most important human helminth infection in terms of morbidity and mortality. A new approach of oral vaccination with DNA-chitosan nanoparticles appears interesting because of their great stability and the ease of target accessibility, besides chitosan immunostimulatory properties. Here we described that chitosan nanoparticles loaded with plasmid DNA encoding Rho1-GTPase protein of Schistosoma mansoni, prepared at different molar ratios of primary amines to DNA phosphate anion (N/P), were able to complex electrostatically with DNA and condense it into positively charged nanostructures. Nanoparticles were able to maintain zeta potential and size characteristics in media that simulate gastric (SGF) and intestinal fluids (SIF). Further in vivo studies showed that oral immunization was not able to induce high levels of specific antibodies but induced high levels of the modulatory cytokine IL-10. This resulted in a significative reduce of liver pathology, although it could not protect mice of infection challenge with S. mansoni worms. Mice immunized only with chitosan nanoparticles presented 47% of protection against parasite infection, suggesting an important role of chitosan in inducing a protective immune response against schistosomiasis, which will be more explored in further studies. |
| format | Article |
| id | doaj-art-f3df5d3c718b4ef1acb3d4701ac82fab |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-f3df5d3c718b4ef1acb3d4701ac82fab2025-08-20T03:25:42ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/938457938457Oral Vaccination Based on DNA-Chitosan Nanoparticles against Schistosoma mansoni InfectionCarolina R. Oliveira0Cíntia M. F. Rezende1Marina R. Silva2Olga M. Borges3Ana P. Pêgo4Alfredo M. Goes5Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos 6627, ICB Q4-167, 31270-901 Belo Horizonte, MG, BrazilDepartamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos 6627, ICB Q4-167, 31270-901 Belo Horizonte, MG, BrazilDepartamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos 6627, ICB Q4-167, 31270-901 Belo Horizonte, MG, BrazilCenter for Neuroscience and Cell Biology (CNC), University of Coimbra, Largo Marquês de Pombal, 3004-517 Coimbra, PortugalInstituto de Engenharia Biomédica (INEB), Universidade do Porto, Rua do Campo Alegre 823, 4150-180 Porto, PortugalDepartamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Presidente Antônio Carlos 6627, ICB Q4-167, 31270-901 Belo Horizonte, MG, BrazilThe development of a vaccine would be essential for the control of schistosomiasis, which is recognized as the most important human helminth infection in terms of morbidity and mortality. A new approach of oral vaccination with DNA-chitosan nanoparticles appears interesting because of their great stability and the ease of target accessibility, besides chitosan immunostimulatory properties. Here we described that chitosan nanoparticles loaded with plasmid DNA encoding Rho1-GTPase protein of Schistosoma mansoni, prepared at different molar ratios of primary amines to DNA phosphate anion (N/P), were able to complex electrostatically with DNA and condense it into positively charged nanostructures. Nanoparticles were able to maintain zeta potential and size characteristics in media that simulate gastric (SGF) and intestinal fluids (SIF). Further in vivo studies showed that oral immunization was not able to induce high levels of specific antibodies but induced high levels of the modulatory cytokine IL-10. This resulted in a significative reduce of liver pathology, although it could not protect mice of infection challenge with S. mansoni worms. Mice immunized only with chitosan nanoparticles presented 47% of protection against parasite infection, suggesting an important role of chitosan in inducing a protective immune response against schistosomiasis, which will be more explored in further studies.http://dx.doi.org/10.1100/2012/938457 |
| spellingShingle | Carolina R. Oliveira Cíntia M. F. Rezende Marina R. Silva Olga M. Borges Ana P. Pêgo Alfredo M. Goes Oral Vaccination Based on DNA-Chitosan Nanoparticles against Schistosoma mansoni Infection The Scientific World Journal |
| title | Oral Vaccination Based on DNA-Chitosan Nanoparticles against Schistosoma mansoni Infection |
| title_full | Oral Vaccination Based on DNA-Chitosan Nanoparticles against Schistosoma mansoni Infection |
| title_fullStr | Oral Vaccination Based on DNA-Chitosan Nanoparticles against Schistosoma mansoni Infection |
| title_full_unstemmed | Oral Vaccination Based on DNA-Chitosan Nanoparticles against Schistosoma mansoni Infection |
| title_short | Oral Vaccination Based on DNA-Chitosan Nanoparticles against Schistosoma mansoni Infection |
| title_sort | oral vaccination based on dna chitosan nanoparticles against schistosoma mansoni infection |
| url | http://dx.doi.org/10.1100/2012/938457 |
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