Activation of Wnt/β-catenin in neural progenitor cells regulates blood–brain barrier development and promotes neuroinflammation
Abstract The central nervous system (CNS) requires specialized blood vessels to support neural function within specific microenvironments. During neurovascular development, endothelial Wnt/β-catenin signaling is required for BBB development within the brain parenchyma, whereas fenestrated blood vess...
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| Format: | Article |
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-85784-8 |
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| author | Dylan J. Sebo Irshad Ali Audrey R. Fetsko Aubrey A. Trimbach Michael R. Taylor |
| author_facet | Dylan J. Sebo Irshad Ali Audrey R. Fetsko Aubrey A. Trimbach Michael R. Taylor |
| author_sort | Dylan J. Sebo |
| collection | DOAJ |
| description | Abstract The central nervous system (CNS) requires specialized blood vessels to support neural function within specific microenvironments. During neurovascular development, endothelial Wnt/β-catenin signaling is required for BBB development within the brain parenchyma, whereas fenestrated blood vessels that lack BBB properties do not require Wnt/β-catenin signaling. Here, we used zebrafish to further characterize this phenotypic heterogeneity of the CNS vasculature. Using transgenic reporters of Wnt/β-catenin transcriptional activity, we found an inverse correlation between activated Wnt/β-catenin signaling in endothelial cells (ECs) versus non-ECs within these distinct microenvironments. Our results indicated that the level of Wnt/β-catenin signaling in non-ECs may regulate Wnt/β-catenin activity in adjacent ECs. To further test this concept, we generated a transgenic Tet-On inducible system to drive constitutively active β-catenin expression in neural progenitor cells (NPCs). We found that dose-dependent activation of Wnt/β-catenin in NPCs caused severe deficiency in CNS angiogenesis and BBB development. Additionally, we discovered a significant increase in the proliferation of microglia and infiltration of peripheral neutrophils indicative of a stereotypical neuroinflammatory response. In conclusion, our results demonstrate the importance of proper Wnt/β-catenin signaling within specific CNS microenvironments and highlights the potentially deleterious consequences of aberrant Wnt activation. |
| format | Article |
| id | doaj-art-f3ab9866c8f749e5bd69a3fabf51b348 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-f3ab9866c8f749e5bd69a3fabf51b3482025-02-02T12:21:36ZengNature PortfolioScientific Reports2045-23222025-01-0115112010.1038/s41598-025-85784-8Activation of Wnt/β-catenin in neural progenitor cells regulates blood–brain barrier development and promotes neuroinflammationDylan J. Sebo0Irshad Ali1Audrey R. Fetsko2Aubrey A. Trimbach3Michael R. Taylor4School of Pharmacy, Division of Pharmaceutical Sciences, University of Wisconsin-MadisonSchool of Pharmacy, Division of Pharmaceutical Sciences, University of Wisconsin-MadisonSchool of Pharmacy, Division of Pharmaceutical Sciences, University of Wisconsin-MadisonSchool of Pharmacy, Division of Pharmaceutical Sciences, University of Wisconsin-MadisonSchool of Pharmacy, Division of Pharmaceutical Sciences, University of Wisconsin-MadisonAbstract The central nervous system (CNS) requires specialized blood vessels to support neural function within specific microenvironments. During neurovascular development, endothelial Wnt/β-catenin signaling is required for BBB development within the brain parenchyma, whereas fenestrated blood vessels that lack BBB properties do not require Wnt/β-catenin signaling. Here, we used zebrafish to further characterize this phenotypic heterogeneity of the CNS vasculature. Using transgenic reporters of Wnt/β-catenin transcriptional activity, we found an inverse correlation between activated Wnt/β-catenin signaling in endothelial cells (ECs) versus non-ECs within these distinct microenvironments. Our results indicated that the level of Wnt/β-catenin signaling in non-ECs may regulate Wnt/β-catenin activity in adjacent ECs. To further test this concept, we generated a transgenic Tet-On inducible system to drive constitutively active β-catenin expression in neural progenitor cells (NPCs). We found that dose-dependent activation of Wnt/β-catenin in NPCs caused severe deficiency in CNS angiogenesis and BBB development. Additionally, we discovered a significant increase in the proliferation of microglia and infiltration of peripheral neutrophils indicative of a stereotypical neuroinflammatory response. In conclusion, our results demonstrate the importance of proper Wnt/β-catenin signaling within specific CNS microenvironments and highlights the potentially deleterious consequences of aberrant Wnt activation.https://doi.org/10.1038/s41598-025-85784-8Blood–Brain barrier (BBB)Endothelial cells (ECs)Neural progenitor cells (NPCs)NeuroinflammationWnt/β-cateninZebrafish |
| spellingShingle | Dylan J. Sebo Irshad Ali Audrey R. Fetsko Aubrey A. Trimbach Michael R. Taylor Activation of Wnt/β-catenin in neural progenitor cells regulates blood–brain barrier development and promotes neuroinflammation Scientific Reports Blood–Brain barrier (BBB) Endothelial cells (ECs) Neural progenitor cells (NPCs) Neuroinflammation Wnt/β-catenin Zebrafish |
| title | Activation of Wnt/β-catenin in neural progenitor cells regulates blood–brain barrier development and promotes neuroinflammation |
| title_full | Activation of Wnt/β-catenin in neural progenitor cells regulates blood–brain barrier development and promotes neuroinflammation |
| title_fullStr | Activation of Wnt/β-catenin in neural progenitor cells regulates blood–brain barrier development and promotes neuroinflammation |
| title_full_unstemmed | Activation of Wnt/β-catenin in neural progenitor cells regulates blood–brain barrier development and promotes neuroinflammation |
| title_short | Activation of Wnt/β-catenin in neural progenitor cells regulates blood–brain barrier development and promotes neuroinflammation |
| title_sort | activation of wnt β catenin in neural progenitor cells regulates blood brain barrier development and promotes neuroinflammation |
| topic | Blood–Brain barrier (BBB) Endothelial cells (ECs) Neural progenitor cells (NPCs) Neuroinflammation Wnt/β-catenin Zebrafish |
| url | https://doi.org/10.1038/s41598-025-85784-8 |
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