Transcriptome Analysis of Long Noncoding RNAs in Toll-Like Receptor 3-Activated Mesenchymal Stem Cells
Mesenchymal stem cells (MSCs) possess great immunomodulatory capacity which lays the foundation for their therapeutic effects in a variety of diseases. Recently, toll-like receptors (TLR) have been shown to modulate MSC functions; however, the underlying molecular mechanisms are poorly understood. E...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/6205485 |
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| author | Shihua Wang Xiaoxia Li Robert Chunhua Zhao |
| author_facet | Shihua Wang Xiaoxia Li Robert Chunhua Zhao |
| author_sort | Shihua Wang |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSCs) possess great immunomodulatory capacity which lays the foundation for their therapeutic effects in a variety of diseases. Recently, toll-like receptors (TLR) have been shown to modulate MSC functions; however, the underlying molecular mechanisms are poorly understood. Emerging evidence suggests that long noncoding RNAs (lncRNAs) are an important class of regulators involved in a wide range of biological processes. To explore the potential involvement of lncRNAs in TLR stimulated MSCs, we performed a comprehensive lncRNA and mRNA profiling through microarray. 10.2% of lncRNAs (1733 out of 16967) and 15.1% of mRNA transcripts (1760 out of 11632) were significantly differentially expressed (absolute fold-change ≥5
, P value ≤0.05) in TLR3 stimulated MSCs. Furthermore, we characterized the differentially expressed lncRNAs through their classes and length distribution and correlated them with differentially expressed mRNA. Here, we are the first to determine genome-wide lncRNAs expression patterns in TLR3 stimulated MSCs by microarray and this work could provide a comprehensive framework of the transcriptome landscapes of TLR3 stimulated MSCs. |
| format | Article |
| id | doaj-art-f3a561d198314733932c59e693af4bbf |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-f3a561d198314733932c59e693af4bbf2025-02-03T05:48:13ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/62054856205485Transcriptome Analysis of Long Noncoding RNAs in Toll-Like Receptor 3-Activated Mesenchymal Stem CellsShihua Wang0Xiaoxia Li1Robert Chunhua Zhao2Center of Excellence in Tissue Engineering, Institute of Basic Medical Sciences and Peking Union Medical College Hospital, School of Basic Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100005, ChinaCenter of Excellence in Tissue Engineering, Institute of Basic Medical Sciences and Peking Union Medical College Hospital, School of Basic Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100005, ChinaCenter of Excellence in Tissue Engineering, Institute of Basic Medical Sciences and Peking Union Medical College Hospital, School of Basic Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100005, ChinaMesenchymal stem cells (MSCs) possess great immunomodulatory capacity which lays the foundation for their therapeutic effects in a variety of diseases. Recently, toll-like receptors (TLR) have been shown to modulate MSC functions; however, the underlying molecular mechanisms are poorly understood. Emerging evidence suggests that long noncoding RNAs (lncRNAs) are an important class of regulators involved in a wide range of biological processes. To explore the potential involvement of lncRNAs in TLR stimulated MSCs, we performed a comprehensive lncRNA and mRNA profiling through microarray. 10.2% of lncRNAs (1733 out of 16967) and 15.1% of mRNA transcripts (1760 out of 11632) were significantly differentially expressed (absolute fold-change ≥5 , P value ≤0.05) in TLR3 stimulated MSCs. Furthermore, we characterized the differentially expressed lncRNAs through their classes and length distribution and correlated them with differentially expressed mRNA. Here, we are the first to determine genome-wide lncRNAs expression patterns in TLR3 stimulated MSCs by microarray and this work could provide a comprehensive framework of the transcriptome landscapes of TLR3 stimulated MSCs.http://dx.doi.org/10.1155/2016/6205485 |
| spellingShingle | Shihua Wang Xiaoxia Li Robert Chunhua Zhao Transcriptome Analysis of Long Noncoding RNAs in Toll-Like Receptor 3-Activated Mesenchymal Stem Cells Stem Cells International |
| title | Transcriptome Analysis of Long Noncoding RNAs in Toll-Like Receptor 3-Activated Mesenchymal Stem Cells |
| title_full | Transcriptome Analysis of Long Noncoding RNAs in Toll-Like Receptor 3-Activated Mesenchymal Stem Cells |
| title_fullStr | Transcriptome Analysis of Long Noncoding RNAs in Toll-Like Receptor 3-Activated Mesenchymal Stem Cells |
| title_full_unstemmed | Transcriptome Analysis of Long Noncoding RNAs in Toll-Like Receptor 3-Activated Mesenchymal Stem Cells |
| title_short | Transcriptome Analysis of Long Noncoding RNAs in Toll-Like Receptor 3-Activated Mesenchymal Stem Cells |
| title_sort | transcriptome analysis of long noncoding rnas in toll like receptor 3 activated mesenchymal stem cells |
| url | http://dx.doi.org/10.1155/2016/6205485 |
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