DNA from <i>Lactobacillus paragasseri</i> SBT2055 Activates Plasmacytoid Dendritic Cells and Induces IFN-α via TLR9
Previously, we reported that <i>Lactobacillus paragasseri</i> SBT2055 (LG2055) activates plasmacytoid dendritic cells (pDCs) and induces interferon alpha (IFN-α) in vitro. Our clinical trial suggested that LG2055 intake may enhance pDC activity, supporting immune maintenance and reducing...
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MDPI AG
2025-06-01
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| Online Access: | https://www.mdpi.com/2076-2607/13/7/1440 |
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| author | Eiji Kobatake Toshinobu Arai |
| author_facet | Eiji Kobatake Toshinobu Arai |
| author_sort | Eiji Kobatake |
| collection | DOAJ |
| description | Previously, we reported that <i>Lactobacillus paragasseri</i> SBT2055 (LG2055) activates plasmacytoid dendritic cells (pDCs) and induces interferon alpha (IFN-α) in vitro. Our clinical trial suggested that LG2055 intake may enhance pDC activity, supporting immune maintenance and reducing subjective common cold symptoms. However, the precise mechanisms remain unclear. In this study, we investigated how LG2055 engages with pDCs to stimulate IFN-α production. We evaluated LG2055-induced pDC activation using flow cytometry, ELISA, and phagocytosis assays. Human peripheral blood mononuclear cells (PBMCs) were stimulated with LG2055 and its components to evaluate immune responses. An in vitro M cell model was used to examine LG2055 translocation. We found that DNA extracted from LG2055 activated pDCs and enhanced IFN-α production via Toll-like receptor 9 (TLR9). Phagocytosis assays demonstrated that LG2055 DNA was internalized by PBMC-derived pDCs, enabling TLR9-mediated signaling. Additionally, LG2055 translocated across M cells in vitro, suggesting potential transport into Peyer’s patches, where it may interact with pDCs. These findings demonstrate that intestinal LG2055 can translocate across M cells, interact with pDCs, and exert immune-stimulatory effects to enhance host antiviral immunity. This study provides mechanistic insight into how dietary components support immune health and could inform the development of novel functional foods or therapeutic strategies. |
| format | Article |
| id | doaj-art-f38d8ff57fed4e7c936090aa0fb0e31b |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-f38d8ff57fed4e7c936090aa0fb0e31b2025-08-20T02:47:14ZengMDPI AGMicroorganisms2076-26072025-06-01137144010.3390/microorganisms13071440DNA from <i>Lactobacillus paragasseri</i> SBT2055 Activates Plasmacytoid Dendritic Cells and Induces IFN-α via TLR9Eiji Kobatake0Toshinobu Arai1Milk Science Research Institute, MEGMILK SNOW BRAND Co., Ltd., Saitama 350-1165, JapanMilk Science Research Institute, MEGMILK SNOW BRAND Co., Ltd., Saitama 350-1165, JapanPreviously, we reported that <i>Lactobacillus paragasseri</i> SBT2055 (LG2055) activates plasmacytoid dendritic cells (pDCs) and induces interferon alpha (IFN-α) in vitro. Our clinical trial suggested that LG2055 intake may enhance pDC activity, supporting immune maintenance and reducing subjective common cold symptoms. However, the precise mechanisms remain unclear. In this study, we investigated how LG2055 engages with pDCs to stimulate IFN-α production. We evaluated LG2055-induced pDC activation using flow cytometry, ELISA, and phagocytosis assays. Human peripheral blood mononuclear cells (PBMCs) were stimulated with LG2055 and its components to evaluate immune responses. An in vitro M cell model was used to examine LG2055 translocation. We found that DNA extracted from LG2055 activated pDCs and enhanced IFN-α production via Toll-like receptor 9 (TLR9). Phagocytosis assays demonstrated that LG2055 DNA was internalized by PBMC-derived pDCs, enabling TLR9-mediated signaling. Additionally, LG2055 translocated across M cells in vitro, suggesting potential transport into Peyer’s patches, where it may interact with pDCs. These findings demonstrate that intestinal LG2055 can translocate across M cells, interact with pDCs, and exert immune-stimulatory effects to enhance host antiviral immunity. This study provides mechanistic insight into how dietary components support immune health and could inform the development of novel functional foods or therapeutic strategies.https://www.mdpi.com/2076-2607/13/7/1440plasmacytoid dendritic cellstype I interferoninterferon-αlactic acid bacteria<i>Lactobacillus paragasseri</i> SBT2055Toll-like receptor 9 |
| spellingShingle | Eiji Kobatake Toshinobu Arai DNA from <i>Lactobacillus paragasseri</i> SBT2055 Activates Plasmacytoid Dendritic Cells and Induces IFN-α via TLR9 Microorganisms plasmacytoid dendritic cells type I interferon interferon-α lactic acid bacteria <i>Lactobacillus paragasseri</i> SBT2055 Toll-like receptor 9 |
| title | DNA from <i>Lactobacillus paragasseri</i> SBT2055 Activates Plasmacytoid Dendritic Cells and Induces IFN-α via TLR9 |
| title_full | DNA from <i>Lactobacillus paragasseri</i> SBT2055 Activates Plasmacytoid Dendritic Cells and Induces IFN-α via TLR9 |
| title_fullStr | DNA from <i>Lactobacillus paragasseri</i> SBT2055 Activates Plasmacytoid Dendritic Cells and Induces IFN-α via TLR9 |
| title_full_unstemmed | DNA from <i>Lactobacillus paragasseri</i> SBT2055 Activates Plasmacytoid Dendritic Cells and Induces IFN-α via TLR9 |
| title_short | DNA from <i>Lactobacillus paragasseri</i> SBT2055 Activates Plasmacytoid Dendritic Cells and Induces IFN-α via TLR9 |
| title_sort | dna from i lactobacillus paragasseri i sbt2055 activates plasmacytoid dendritic cells and induces ifn α via tlr9 |
| topic | plasmacytoid dendritic cells type I interferon interferon-α lactic acid bacteria <i>Lactobacillus paragasseri</i> SBT2055 Toll-like receptor 9 |
| url | https://www.mdpi.com/2076-2607/13/7/1440 |
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