CircMYO9A inhibits influenza A virus replication by dampening haemagglutinin cleavage via increasing SERPINE1/PAI-1 expression
Circular RNAs (circRNAs) represent a class of widespread and diverse covalently closed circular endogenous RNAs that play critical roles in regulating gene expression in mammals. However, the roles and regulatory mechanisms of circRNAs during influenza A virus (IAV) infection remain largely unexplor...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2025.2502007 |
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| author | Yiqing Zheng Xiaoting Zhang Zhiyuan Liu Menglu Fan Lulu Deng Jihui Ping |
| author_facet | Yiqing Zheng Xiaoting Zhang Zhiyuan Liu Menglu Fan Lulu Deng Jihui Ping |
| author_sort | Yiqing Zheng |
| collection | DOAJ |
| description | Circular RNAs (circRNAs) represent a class of widespread and diverse covalently closed circular endogenous RNAs that play critical roles in regulating gene expression in mammals. However, the roles and regulatory mechanisms of circRNAs during influenza A virus (IAV) infection remain largely unexplored. In this study, we screened the circRNA transcription profiles of WSN-infected cells to identify circRNAs involved in viral replication and identified a novel differentially expressed circular RNA, circMYO9A. Mechanistically, circMYO9A acts as a competing endogenous RNA (ceRNA) for SERPINE1/PAI-1 by sponging miR-6059-3p, thereby increasing SERPINE1/PAI-1 expression, which restricts IAV haemagglutinin cleavage and subsequently reduces the infectivity of progeny viruses. Importantly, our findings demonstrate that circMYO9A significantly inhibits viral replication in the lungs of infected mice, potentially increasing their survival during IAV infection. These results demonstrate that circRNAs play crucial roles in inhibiting IAV replication and provide novel insights into potential therapeutic strategies involving circRNAs. |
| format | Article |
| id | doaj-art-f367955a5e034cffae82de25614cab4c |
| institution | OA Journals |
| issn | 2222-1751 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-f367955a5e034cffae82de25614cab4c2025-08-20T01:55:21ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512025-12-0114110.1080/22221751.2025.2502007CircMYO9A inhibits influenza A virus replication by dampening haemagglutinin cleavage via increasing SERPINE1/PAI-1 expressionYiqing Zheng0Xiaoting Zhang1Zhiyuan Liu2Menglu Fan3Lulu Deng4Jihui Ping5MOE Joint International Research Laboratory of Animal Health and Food Safety, Engineering Center of Animal Immunity of Jiangsu Province, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People’s Republic of ChinaMOE Joint International Research Laboratory of Animal Health and Food Safety, Engineering Center of Animal Immunity of Jiangsu Province, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People’s Republic of ChinaMOE Joint International Research Laboratory of Animal Health and Food Safety, Engineering Center of Animal Immunity of Jiangsu Province, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People’s Republic of ChinaMOE Joint International Research Laboratory of Animal Health and Food Safety, Engineering Center of Animal Immunity of Jiangsu Province, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People’s Republic of ChinaMOE Joint International Research Laboratory of Animal Health and Food Safety, Engineering Center of Animal Immunity of Jiangsu Province, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People’s Republic of ChinaMOE Joint International Research Laboratory of Animal Health and Food Safety, Engineering Center of Animal Immunity of Jiangsu Province, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, People’s Republic of ChinaCircular RNAs (circRNAs) represent a class of widespread and diverse covalently closed circular endogenous RNAs that play critical roles in regulating gene expression in mammals. However, the roles and regulatory mechanisms of circRNAs during influenza A virus (IAV) infection remain largely unexplored. In this study, we screened the circRNA transcription profiles of WSN-infected cells to identify circRNAs involved in viral replication and identified a novel differentially expressed circular RNA, circMYO9A. Mechanistically, circMYO9A acts as a competing endogenous RNA (ceRNA) for SERPINE1/PAI-1 by sponging miR-6059-3p, thereby increasing SERPINE1/PAI-1 expression, which restricts IAV haemagglutinin cleavage and subsequently reduces the infectivity of progeny viruses. Importantly, our findings demonstrate that circMYO9A significantly inhibits viral replication in the lungs of infected mice, potentially increasing their survival during IAV infection. These results demonstrate that circRNAs play crucial roles in inhibiting IAV replication and provide novel insights into potential therapeutic strategies involving circRNAs.https://www.tandfonline.com/doi/10.1080/22221751.2025.2502007Influenza viruscircMYO9ASERPINE1CeRNAhaemagglutinin cleavage |
| spellingShingle | Yiqing Zheng Xiaoting Zhang Zhiyuan Liu Menglu Fan Lulu Deng Jihui Ping CircMYO9A inhibits influenza A virus replication by dampening haemagglutinin cleavage via increasing SERPINE1/PAI-1 expression Emerging Microbes and Infections Influenza virus circMYO9A SERPINE1 CeRNA haemagglutinin cleavage |
| title | CircMYO9A inhibits influenza A virus replication by dampening haemagglutinin cleavage via increasing SERPINE1/PAI-1 expression |
| title_full | CircMYO9A inhibits influenza A virus replication by dampening haemagglutinin cleavage via increasing SERPINE1/PAI-1 expression |
| title_fullStr | CircMYO9A inhibits influenza A virus replication by dampening haemagglutinin cleavage via increasing SERPINE1/PAI-1 expression |
| title_full_unstemmed | CircMYO9A inhibits influenza A virus replication by dampening haemagglutinin cleavage via increasing SERPINE1/PAI-1 expression |
| title_short | CircMYO9A inhibits influenza A virus replication by dampening haemagglutinin cleavage via increasing SERPINE1/PAI-1 expression |
| title_sort | circmyo9a inhibits influenza a virus replication by dampening haemagglutinin cleavage via increasing serpine1 pai 1 expression |
| topic | Influenza virus circMYO9A SERPINE1 CeRNA haemagglutinin cleavage |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2025.2502007 |
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