Lactobacillus plantarum in high methoxylated pectin-based encapsulation systems supports gut epithelial wound healing in vitro
Wound healing and gut epithelial barrier regulation are crucial for intestinal homeostasis. Effective repair of gut epithelial wounds is crucial for re-establishing the mucosal barrier and resolving inflammation. Pectins, versatile polysaccharides in food, protect sensitive components, such as livin...
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Elsevier
2025-06-01
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| Series: | Food Hydrocolloids for Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266702592500024X |
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| author | Felipe Galvez-Jiron Lucas de Freitas Pedrosa Luis Silva Lagos Xin Tang Traudy Wandersleben Rodrigo Navia Stephan Drusch Francisca Acevedo Paul de Vos |
| author_facet | Felipe Galvez-Jiron Lucas de Freitas Pedrosa Luis Silva Lagos Xin Tang Traudy Wandersleben Rodrigo Navia Stephan Drusch Francisca Acevedo Paul de Vos |
| author_sort | Felipe Galvez-Jiron |
| collection | DOAJ |
| description | Wound healing and gut epithelial barrier regulation are crucial for intestinal homeostasis. Effective repair of gut epithelial wounds is crucial for re-establishing the mucosal barrier and resolving inflammation. Pectins, versatile polysaccharides in food, protect sensitive components, such as living bacteria, during gastrointestinal transit and support wound healing by promoting cell proliferation and migration.This study investigated the effects of encapsulated Lactobacillus plantarum WCFS1 on cell proliferation and migration during epithelial wound healing, using pectin-based beads stabilized with alginate or chitosan. Pectin from lemon, with two degrees of methyl esterification (DM18 and DM88), produced four microbead types. T84 cells were incubated with these beads for 24 hours. The results revealed that cell proliferation and migration were influenced by the bead type, the degree of methyl esterification of the pectin, and the inclusion of L. plantarum. Empty DM88 pectin beads improved cell proliferation while reducing migration tendencies, whereas DM18 beads delayed migration.Beads containing L. plantarum demonstrated different effects: DM18 beads enhanced proliferation without inhibiting migration, while DM88 beads reduced migration. Both empty and L. plantarum-loaded beads decreased CCL20 production. Alginate/pectin beads with L. plantarum increased IL-8, while chitosan/pectin beads delayed migration, reduced CCL20 secretion, and modulated IL-8 levels.In conclusion, DM88 pectin beads without L. plantarum and DM18 beads containing L. plantarum supported cell proliferation over migration during epithelial wound healing. These findings suggest that pectin-based encapsulation systems may play a role in promoting gut health, protecting barrier integrity, and delivering therapeutic agents. |
| format | Article |
| id | doaj-art-f34d5ca0c4e84dc7af128e3c613d2e4e |
| institution | DOAJ |
| issn | 2667-0259 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Hydrocolloids for Health |
| spelling | doaj-art-f34d5ca0c4e84dc7af128e3c613d2e4e2025-08-20T03:08:17ZengElsevierFood Hydrocolloids for Health2667-02592025-06-01710021810.1016/j.fhfh.2025.100218Lactobacillus plantarum in high methoxylated pectin-based encapsulation systems supports gut epithelial wound healing in vitroFelipe Galvez-Jiron0Lucas de Freitas Pedrosa1Luis Silva Lagos2Xin Tang3Traudy Wandersleben4Rodrigo Navia5Stephan Drusch6Francisca Acevedo7Paul de Vos8Immunoendocrinology, Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands; Doctorado en Ciencias Mención Biología Celular y Molecular Aplicada, Facultad de Medicina, Universidad de La Frontera, Temuco, 4811230, Chile; Millennium Nucleus Bioproducts, Genomics and Environmental Microbiology (BioGEM), Avenida España 1680, 2390123, Valparaíso, Chile; Corresponding author at: Hanzeplein 1, EA11, 9713 GZ, Groningen, the Netherlands.Immunoendocrinology, Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands; Department of Food Science and Experimental Nutrition, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, BrazilImmunoendocrinology, Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, the NetherlandsImmunoendocrinology, Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, the NetherlandsDepartamento de Ciencias Químicas y Recursos Naturales, Facultad de Ingeniería y Ciencias, Universidad de La Frontera, Avenida Francisco Salazar, 01145, Temuco, ChileMillennium Nucleus Bioproducts, Genomics and Environmental Microbiology (BioGEM), Avenida España 1680, 2390123, Valparaíso, Chile; Facultad de Ingeniería, Universidad San Sebastián, Bellavista 7, Santiago, Chile & Centre for Biotechnology and Bioengineering (CeBiB), AFB240001, ChileTechnische Universität Berlin, Institute of Food Technology and Food Chemistry, Department of Food Technology and Food Material Science, Königin-Luise-Str. 22, 14195, Berlin, GermanyMillennium Nucleus Bioproducts, Genomics and Environmental Microbiology (BioGEM), Avenida España 1680, 2390123, Valparaíso, Chile; Center of Excellence in Traslational Medicine (CEMT), Faculty of Medicine, and Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Casilla 54-D, Temuco, Chile; Department of Basic Sciences, Faculty of Medicine, Universidad de La Frontera, Casilla 54-D, Temuco, ChileImmunoendocrinology, Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands; Centre for Healthy Eating & Food Innovation (HEFI), Maastricht University - campus Venlo, 5928, SZ, Venlo, the NetherlandsWound healing and gut epithelial barrier regulation are crucial for intestinal homeostasis. Effective repair of gut epithelial wounds is crucial for re-establishing the mucosal barrier and resolving inflammation. Pectins, versatile polysaccharides in food, protect sensitive components, such as living bacteria, during gastrointestinal transit and support wound healing by promoting cell proliferation and migration.This study investigated the effects of encapsulated Lactobacillus plantarum WCFS1 on cell proliferation and migration during epithelial wound healing, using pectin-based beads stabilized with alginate or chitosan. Pectin from lemon, with two degrees of methyl esterification (DM18 and DM88), produced four microbead types. T84 cells were incubated with these beads for 24 hours. The results revealed that cell proliferation and migration were influenced by the bead type, the degree of methyl esterification of the pectin, and the inclusion of L. plantarum. Empty DM88 pectin beads improved cell proliferation while reducing migration tendencies, whereas DM18 beads delayed migration.Beads containing L. plantarum demonstrated different effects: DM18 beads enhanced proliferation without inhibiting migration, while DM88 beads reduced migration. Both empty and L. plantarum-loaded beads decreased CCL20 production. Alginate/pectin beads with L. plantarum increased IL-8, while chitosan/pectin beads delayed migration, reduced CCL20 secretion, and modulated IL-8 levels.In conclusion, DM88 pectin beads without L. plantarum and DM18 beads containing L. plantarum supported cell proliferation over migration during epithelial wound healing. These findings suggest that pectin-based encapsulation systems may play a role in promoting gut health, protecting barrier integrity, and delivering therapeutic agents.http://www.sciencedirect.com/science/article/pii/S266702592500024XPectin beadsProliferationMigrationInterleukin 8CC chemokine ligand 20 |
| spellingShingle | Felipe Galvez-Jiron Lucas de Freitas Pedrosa Luis Silva Lagos Xin Tang Traudy Wandersleben Rodrigo Navia Stephan Drusch Francisca Acevedo Paul de Vos Lactobacillus plantarum in high methoxylated pectin-based encapsulation systems supports gut epithelial wound healing in vitro Food Hydrocolloids for Health Pectin beads Proliferation Migration Interleukin 8 CC chemokine ligand 20 |
| title | Lactobacillus plantarum in high methoxylated pectin-based encapsulation systems supports gut epithelial wound healing in vitro |
| title_full | Lactobacillus plantarum in high methoxylated pectin-based encapsulation systems supports gut epithelial wound healing in vitro |
| title_fullStr | Lactobacillus plantarum in high methoxylated pectin-based encapsulation systems supports gut epithelial wound healing in vitro |
| title_full_unstemmed | Lactobacillus plantarum in high methoxylated pectin-based encapsulation systems supports gut epithelial wound healing in vitro |
| title_short | Lactobacillus plantarum in high methoxylated pectin-based encapsulation systems supports gut epithelial wound healing in vitro |
| title_sort | lactobacillus plantarum in high methoxylated pectin based encapsulation systems supports gut epithelial wound healing in vitro |
| topic | Pectin beads Proliferation Migration Interleukin 8 CC chemokine ligand 20 |
| url | http://www.sciencedirect.com/science/article/pii/S266702592500024X |
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