Assessment of line differentiation in the Thoroughbred horse breed using DNA microsatellite loci
The Thoroughbred (TB) horse is the best racehorse breed used in the racing industry. This breed has had a closed studbook for about 300 years. In Russia TB horses have been bred since the second half of the XVIII century. The modern Russian Stud Book register of TB horses is partially presented by s...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2019-08-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/2207 |
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| author | L. A. Khrabrova N. V. Blohina O. I. Suleymanov G. А. Rozhdestvenskaya V. F. Pustovoy |
| author_facet | L. A. Khrabrova N. V. Blohina O. I. Suleymanov G. А. Rozhdestvenskaya V. F. Pustovoy |
| author_sort | L. A. Khrabrova |
| collection | DOAJ |
| description | The Thoroughbred (TB) horse is the best racehorse breed used in the racing industry. This breed has had a closed studbook for about 300 years. In Russia TB horses have been bred since the second half of the XVIII century. The modern Russian Stud Book register of TB horses is partially presented by stallions and broodmares imported from different countries. The genealogical structure of the breed is represented by 17 lines, among which the Northern Dancer line dominates (30.9 %). The study of features of different lines of TB was carried out on 17 loci of DNA microsatellites (VHL20, HTG4, AHT4, HMS7, HTG6, AHT5, HMS6, ASB23, ASB2, HTG10, HTG7, HMS3, HMS2, ASB17, LEX3, HMS1 and CA425) to assess genetic differentiation of the genealogical structure. The results of the DNA typing of 8091 Thoroughbred horses across microsatellite loci show that the gene pool of the domestic population is represented by 100 alleles typical of the breed. A comparative analysis of the genotypes of horses representing different lines indicates that they differ in the number of alleles (85–99), allele frequencies, the level of polymorphism Ae (2.93–3.48) and the degree of the observed heterozygosity Ho (0.653–0.739). The genetic distances between the lines varied in a wide range from 0.014 (Nasrullah – Northern Dancer) to 0.125 (Massine – Teddy). The correspondence to HWE was maintained in most lines, which is confirmed by the negative values of Fis. Cluster analysis demonstrated the correspondence of the obtained dendrogram of Nei’s genetic distances to its genealogical scheme of lines. Genetic differentiation of lines by index Fst varied in a range 0.005–0.073 at the mean value Fst = 0.024. The data indicate genetic differentiation of lines of TB at the STR markers and confirm the effectiveness of the system of linear breeding for the maintenance of interbreed biodiversity. |
| format | Article |
| id | doaj-art-f34a1bbb2f304695ba067b8a738f6993 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2019-08-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-f34a1bbb2f304695ba067b8a738f69932025-02-01T09:58:07ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592019-08-0123556957410.18699/VJ19.526944Assessment of line differentiation in the Thoroughbred horse breed using DNA microsatellite lociL. A. Khrabrova0N. V. Blohina1O. I. Suleymanov2G. А. Rozhdestvenskaya3V. F. Pustovoy4All-Russian Research Institute for Horse BreedingAll-Russian Research Institute for Horse BreedingAll-Russian Research Institute for Horse BreedingAll-Russian Research Institute for Horse BreedingAll-Russian Research Institute for Horse BreedingThe Thoroughbred (TB) horse is the best racehorse breed used in the racing industry. This breed has had a closed studbook for about 300 years. In Russia TB horses have been bred since the second half of the XVIII century. The modern Russian Stud Book register of TB horses is partially presented by stallions and broodmares imported from different countries. The genealogical structure of the breed is represented by 17 lines, among which the Northern Dancer line dominates (30.9 %). The study of features of different lines of TB was carried out on 17 loci of DNA microsatellites (VHL20, HTG4, AHT4, HMS7, HTG6, AHT5, HMS6, ASB23, ASB2, HTG10, HTG7, HMS3, HMS2, ASB17, LEX3, HMS1 and CA425) to assess genetic differentiation of the genealogical structure. The results of the DNA typing of 8091 Thoroughbred horses across microsatellite loci show that the gene pool of the domestic population is represented by 100 alleles typical of the breed. A comparative analysis of the genotypes of horses representing different lines indicates that they differ in the number of alleles (85–99), allele frequencies, the level of polymorphism Ae (2.93–3.48) and the degree of the observed heterozygosity Ho (0.653–0.739). The genetic distances between the lines varied in a wide range from 0.014 (Nasrullah – Northern Dancer) to 0.125 (Massine – Teddy). The correspondence to HWE was maintained in most lines, which is confirmed by the negative values of Fis. Cluster analysis demonstrated the correspondence of the obtained dendrogram of Nei’s genetic distances to its genealogical scheme of lines. Genetic differentiation of lines by index Fst varied in a range 0.005–0.073 at the mean value Fst = 0.024. The data indicate genetic differentiation of lines of TB at the STR markers and confirm the effectiveness of the system of linear breeding for the maintenance of interbreed biodiversity.https://vavilov.elpub.ru/jour/article/view/2207genetic diversityhorselinear structuremicrosatellite dnapopulation analysisthoroughbred |
| spellingShingle | L. A. Khrabrova N. V. Blohina O. I. Suleymanov G. А. Rozhdestvenskaya V. F. Pustovoy Assessment of line differentiation in the Thoroughbred horse breed using DNA microsatellite loci Вавиловский журнал генетики и селекции genetic diversity horse linear structure microsatellite dna population analysis thoroughbred |
| title | Assessment of line differentiation in the Thoroughbred horse breed using DNA microsatellite loci |
| title_full | Assessment of line differentiation in the Thoroughbred horse breed using DNA microsatellite loci |
| title_fullStr | Assessment of line differentiation in the Thoroughbred horse breed using DNA microsatellite loci |
| title_full_unstemmed | Assessment of line differentiation in the Thoroughbred horse breed using DNA microsatellite loci |
| title_short | Assessment of line differentiation in the Thoroughbred horse breed using DNA microsatellite loci |
| title_sort | assessment of line differentiation in the thoroughbred horse breed using dna microsatellite loci |
| topic | genetic diversity horse linear structure microsatellite dna population analysis thoroughbred |
| url | https://vavilov.elpub.ru/jour/article/view/2207 |
| work_keys_str_mv | AT lakhrabrova assessmentoflinedifferentiationinthethoroughbredhorsebreedusingdnamicrosatelliteloci AT nvblohina assessmentoflinedifferentiationinthethoroughbredhorsebreedusingdnamicrosatelliteloci AT oisuleymanov assessmentoflinedifferentiationinthethoroughbredhorsebreedusingdnamicrosatelliteloci AT garozhdestvenskaya assessmentoflinedifferentiationinthethoroughbredhorsebreedusingdnamicrosatelliteloci AT vfpustovoy assessmentoflinedifferentiationinthethoroughbredhorsebreedusingdnamicrosatelliteloci |