Alleviation of DSS-induced colitis by Meconopsis polysaccharides correlated with reduced PI3K/AKT signaling and gut microbiome diversity
IntroductionInflammatory bowel disease (IBD) is a recurrent gastrointestinal disorder that significantly impacts patients’ quality of life globally. This study focuses on the polysaccharides (MP) extracted from Meconopsis integrifolia, to investigate its role in alleviating DSS (dextran sulfate sodi...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-02-01
|
| Series: | Frontiers in Pharmacology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2025.1459668/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823856547058417664 |
|---|---|
| author | Jun Dai Weidong Wang Fangfang He Yujuan Wang Denglang Zou |
| author_facet | Jun Dai Weidong Wang Fangfang He Yujuan Wang Denglang Zou |
| author_sort | Jun Dai |
| collection | DOAJ |
| description | IntroductionInflammatory bowel disease (IBD) is a recurrent gastrointestinal disorder that significantly impacts patients’ quality of life globally. This study focuses on the polysaccharides (MP) extracted from Meconopsis integrifolia, to investigate its role in alleviating DSS (dextran sulfate sodium)-induced colitis in mice.MethodsThe study commenced with a comprehensive chemical characterization of Meconopsis polysaccharides. Subsequently, the colitis-alleviating activity of MP was validated through in vivo experiments.ResultsThe results revealed that MP is primarily composed of ten monosaccharides, exhibits good thermal stability, and has a relatively uniform molecular weight distribution. In vivo experiments demonstrated that MP significantly mitigated DSS-induced weight loss, increased DAI, colon shortening, and tissue damage in mice. Furthermore, MP reduced the levels of inflammatory cytokines such as IL-1β, TNF-α, and IL-6 in serum. Mechanistically, MP exerted its anti-inflammatory effects by inhibiting the activation of the PI3K/AKT signaling pathway. Additionally, MP promoted gut microbiota diversity and regulated SCFA concentrations, contributing to an improved intestinal microenvironment and alleviation of colitis symptoms.DiscussionOur findings highlight the superior effectiveness of Meconopsis polysaccharides in alleviating DSS-induced colitis and open new avenues for targeted therapeutic strategies in the treatment of IBD. |
| format | Article |
| id | doaj-art-f340d511fbe34e9ea969ae103939bc05 |
| institution | Kabale University |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj-art-f340d511fbe34e9ea969ae103939bc052025-02-12T07:26:34ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-02-011610.3389/fphar.2025.14596681459668Alleviation of DSS-induced colitis by Meconopsis polysaccharides correlated with reduced PI3K/AKT signaling and gut microbiome diversityJun Dai0Weidong Wang1Fangfang He2Yujuan Wang3Denglang Zou4Engineering technology research center of Plant Cell Engineering, West AnHui University, Lu’an, ChinaCollege of Pharmacy, Hubei University of Chinese Medicine, Wuhan, ChinaCollege of Pharmacy, Hubei University of Chinese Medicine, Wuhan, ChinaEngineering technology research center of Plant Cell Engineering, West AnHui University, Lu’an, ChinaCollege of Pharmacy, Hubei University of Chinese Medicine, Wuhan, ChinaIntroductionInflammatory bowel disease (IBD) is a recurrent gastrointestinal disorder that significantly impacts patients’ quality of life globally. This study focuses on the polysaccharides (MP) extracted from Meconopsis integrifolia, to investigate its role in alleviating DSS (dextran sulfate sodium)-induced colitis in mice.MethodsThe study commenced with a comprehensive chemical characterization of Meconopsis polysaccharides. Subsequently, the colitis-alleviating activity of MP was validated through in vivo experiments.ResultsThe results revealed that MP is primarily composed of ten monosaccharides, exhibits good thermal stability, and has a relatively uniform molecular weight distribution. In vivo experiments demonstrated that MP significantly mitigated DSS-induced weight loss, increased DAI, colon shortening, and tissue damage in mice. Furthermore, MP reduced the levels of inflammatory cytokines such as IL-1β, TNF-α, and IL-6 in serum. Mechanistically, MP exerted its anti-inflammatory effects by inhibiting the activation of the PI3K/AKT signaling pathway. Additionally, MP promoted gut microbiota diversity and regulated SCFA concentrations, contributing to an improved intestinal microenvironment and alleviation of colitis symptoms.DiscussionOur findings highlight the superior effectiveness of Meconopsis polysaccharides in alleviating DSS-induced colitis and open new avenues for targeted therapeutic strategies in the treatment of IBD.https://www.frontiersin.org/articles/10.3389/fphar.2025.1459668/fullMeconopsis polysaccharidesDSS-induced colitisPI3K/Akt signaling pathwaygut microbiome diversityshort-chain fatty acids |
| spellingShingle | Jun Dai Weidong Wang Fangfang He Yujuan Wang Denglang Zou Alleviation of DSS-induced colitis by Meconopsis polysaccharides correlated with reduced PI3K/AKT signaling and gut microbiome diversity Frontiers in Pharmacology Meconopsis polysaccharides DSS-induced colitis PI3K/Akt signaling pathway gut microbiome diversity short-chain fatty acids |
| title | Alleviation of DSS-induced colitis by Meconopsis polysaccharides correlated with reduced PI3K/AKT signaling and gut microbiome diversity |
| title_full | Alleviation of DSS-induced colitis by Meconopsis polysaccharides correlated with reduced PI3K/AKT signaling and gut microbiome diversity |
| title_fullStr | Alleviation of DSS-induced colitis by Meconopsis polysaccharides correlated with reduced PI3K/AKT signaling and gut microbiome diversity |
| title_full_unstemmed | Alleviation of DSS-induced colitis by Meconopsis polysaccharides correlated with reduced PI3K/AKT signaling and gut microbiome diversity |
| title_short | Alleviation of DSS-induced colitis by Meconopsis polysaccharides correlated with reduced PI3K/AKT signaling and gut microbiome diversity |
| title_sort | alleviation of dss induced colitis by meconopsis polysaccharides correlated with reduced pi3k akt signaling and gut microbiome diversity |
| topic | Meconopsis polysaccharides DSS-induced colitis PI3K/Akt signaling pathway gut microbiome diversity short-chain fatty acids |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2025.1459668/full |
| work_keys_str_mv | AT jundai alleviationofdssinducedcolitisbymeconopsispolysaccharidescorrelatedwithreducedpi3kaktsignalingandgutmicrobiomediversity AT weidongwang alleviationofdssinducedcolitisbymeconopsispolysaccharidescorrelatedwithreducedpi3kaktsignalingandgutmicrobiomediversity AT fangfanghe alleviationofdssinducedcolitisbymeconopsispolysaccharidescorrelatedwithreducedpi3kaktsignalingandgutmicrobiomediversity AT yujuanwang alleviationofdssinducedcolitisbymeconopsispolysaccharidescorrelatedwithreducedpi3kaktsignalingandgutmicrobiomediversity AT denglangzou alleviationofdssinducedcolitisbymeconopsispolysaccharidescorrelatedwithreducedpi3kaktsignalingandgutmicrobiomediversity |