Shionone Relieves Urinary Tract Infections by Removing Bacteria from Bladder Epithelial Cells
In clinical practice, urinary tract infections (UTIs) are second only to respiratory infections in terms of infectious diseases. In recent years, drug resistance of Escherichia coli (E. coli) has increased significantly. The therapeutic effects of Shionone on UTI were assessed by modelling UTI in SD...
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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Cellular Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2023/3201540 |
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| author | Hao Yin Jiaoli Zhu Yi Jiang Yijing Mao Chenquan Tang Hui Cao Yufang Huang Huijun Zhu Jianping Luo Qingjiang Jin Qinglei Jin Yi Xue Xin Wang |
| author_facet | Hao Yin Jiaoli Zhu Yi Jiang Yijing Mao Chenquan Tang Hui Cao Yufang Huang Huijun Zhu Jianping Luo Qingjiang Jin Qinglei Jin Yi Xue Xin Wang |
| author_sort | Hao Yin |
| collection | DOAJ |
| description | In clinical practice, urinary tract infections (UTIs) are second only to respiratory infections in terms of infectious diseases. In recent years, drug resistance of Escherichia coli (E. coli) has increased significantly. The therapeutic effects of Shionone on UTI were assessed by modelling UTI in SD rats and SV-HUC-1 cells with E. coli solution. After treatment of Shionone, the UTI rat model showed a decrease in wet weight/body weight of bladder, as well as a reduction in cellular inflammatory infiltration of bladder tissue and a decrease in urinary levels of IL-6, IL-1β, and TNF-α. In addition, the levels of proinflammatory factors were significantly reduced in a dose-dependent manner in UTI cell model treated with different doses of Shionone (5, 10, and 20 μg/kg). The results of immunofluorescence analysis in both in vivo and in vitro experiments revealed that Shionone reduced bacterial load and the number of E. coli colonies growing on the plates was greatly reduced. These results suggested that Shionone has a good therapeutic effect on UTI, achieved by reducing bacterial load in bladder epithelial cells. The data presented here provide a basis for further research into the treatment of UTI. |
| format | Article |
| id | doaj-art-f329f129b7cc4dae9141f9df460ce602 |
| institution | Kabale University |
| issn | 1462-5822 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cellular Microbiology |
| spelling | doaj-art-f329f129b7cc4dae9141f9df460ce6022025-02-03T01:29:51ZengWileyCellular Microbiology1462-58222023-01-01202310.1155/2023/3201540Shionone Relieves Urinary Tract Infections by Removing Bacteria from Bladder Epithelial CellsHao Yin0Jiaoli Zhu1Yi Jiang2Yijing Mao3Chenquan Tang4Hui Cao5Yufang Huang6Huijun Zhu7Jianping Luo8Qingjiang Jin9Qinglei Jin10Yi Xue11Xin Wang12Department of NephrologyDepartment of NephrologyDepartment of NephrologyDepartment of NephrologyDepartment of NephrologyDepartment of NephrologyDepartment of NephrologyDepartment of NephrologyDepartment of NephrologyDepartment of NephrologyDepartment of NephrologyDepartment of NephrologyDepartment of NephrologyIn clinical practice, urinary tract infections (UTIs) are second only to respiratory infections in terms of infectious diseases. In recent years, drug resistance of Escherichia coli (E. coli) has increased significantly. The therapeutic effects of Shionone on UTI were assessed by modelling UTI in SD rats and SV-HUC-1 cells with E. coli solution. After treatment of Shionone, the UTI rat model showed a decrease in wet weight/body weight of bladder, as well as a reduction in cellular inflammatory infiltration of bladder tissue and a decrease in urinary levels of IL-6, IL-1β, and TNF-α. In addition, the levels of proinflammatory factors were significantly reduced in a dose-dependent manner in UTI cell model treated with different doses of Shionone (5, 10, and 20 μg/kg). The results of immunofluorescence analysis in both in vivo and in vitro experiments revealed that Shionone reduced bacterial load and the number of E. coli colonies growing on the plates was greatly reduced. These results suggested that Shionone has a good therapeutic effect on UTI, achieved by reducing bacterial load in bladder epithelial cells. The data presented here provide a basis for further research into the treatment of UTI.http://dx.doi.org/10.1155/2023/3201540 |
| spellingShingle | Hao Yin Jiaoli Zhu Yi Jiang Yijing Mao Chenquan Tang Hui Cao Yufang Huang Huijun Zhu Jianping Luo Qingjiang Jin Qinglei Jin Yi Xue Xin Wang Shionone Relieves Urinary Tract Infections by Removing Bacteria from Bladder Epithelial Cells Cellular Microbiology |
| title | Shionone Relieves Urinary Tract Infections by Removing Bacteria from Bladder Epithelial Cells |
| title_full | Shionone Relieves Urinary Tract Infections by Removing Bacteria from Bladder Epithelial Cells |
| title_fullStr | Shionone Relieves Urinary Tract Infections by Removing Bacteria from Bladder Epithelial Cells |
| title_full_unstemmed | Shionone Relieves Urinary Tract Infections by Removing Bacteria from Bladder Epithelial Cells |
| title_short | Shionone Relieves Urinary Tract Infections by Removing Bacteria from Bladder Epithelial Cells |
| title_sort | shionone relieves urinary tract infections by removing bacteria from bladder epithelial cells |
| url | http://dx.doi.org/10.1155/2023/3201540 |
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