Laser Ablation Electrospray Ionization Time-of-Flight Mass Spectrometry for Direct Analysis of Biological Tissue
Direct analysis and identification of biological tissue is significant for clinical applications. In this study, porcine liver and kidney have been analyzed using laser ablation electrospray ionization time-of-flight mass spectrometry (LAESI-TOFMS). This method showed good reproducibility for the sa...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2019/1417035 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850164403438092288 |
|---|---|
| author | Wenzhao Zhou Yan Hong Chaoqun Huang Chengyin Shen Yannan Chu |
| author_facet | Wenzhao Zhou Yan Hong Chaoqun Huang Chengyin Shen Yannan Chu |
| author_sort | Wenzhao Zhou |
| collection | DOAJ |
| description | Direct analysis and identification of biological tissue is significant for clinical applications. In this study, porcine liver and kidney have been analyzed using laser ablation electrospray ionization time-of-flight mass spectrometry (LAESI-TOFMS). This method showed good reproducibility for the same types of tissue and is capable of distinguishing different tissue species. The margin assessment was also performed using porcine renal tissue, and the response time was less than 6 s. Furthermore, human hepatocarcinoma tissue and normal tissue were identified using this method. Our results indicate that LAESI-TOFMS is a feasible approach for direct identification of tumor tissue and potential for assessment of the resection margin. |
| format | Article |
| id | doaj-art-f2ea195ad7d949769a2fe750bbf49721 |
| institution | OA Journals |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-f2ea195ad7d949769a2fe750bbf497212025-08-20T02:21:59ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732019-01-01201910.1155/2019/14170351417035Laser Ablation Electrospray Ionization Time-of-Flight Mass Spectrometry for Direct Analysis of Biological TissueWenzhao Zhou0Yan Hong1Chaoqun Huang2Chengyin Shen3Yannan Chu4Anhui Province Key Laboratory of Medical Physics and Technology, Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, ChinaSchool of Electrical and Information Engineering, Anhui University of Science and Technology, Huainan, Anhui 232001, ChinaAnhui Province Key Laboratory of Medical Physics and Technology, Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, ChinaAnhui Province Key Laboratory of Medical Physics and Technology, Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, ChinaAnhui Province Key Laboratory of Medical Physics and Technology, Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, ChinaDirect analysis and identification of biological tissue is significant for clinical applications. In this study, porcine liver and kidney have been analyzed using laser ablation electrospray ionization time-of-flight mass spectrometry (LAESI-TOFMS). This method showed good reproducibility for the same types of tissue and is capable of distinguishing different tissue species. The margin assessment was also performed using porcine renal tissue, and the response time was less than 6 s. Furthermore, human hepatocarcinoma tissue and normal tissue were identified using this method. Our results indicate that LAESI-TOFMS is a feasible approach for direct identification of tumor tissue and potential for assessment of the resection margin.http://dx.doi.org/10.1155/2019/1417035 |
| spellingShingle | Wenzhao Zhou Yan Hong Chaoqun Huang Chengyin Shen Yannan Chu Laser Ablation Electrospray Ionization Time-of-Flight Mass Spectrometry for Direct Analysis of Biological Tissue Journal of Analytical Methods in Chemistry |
| title | Laser Ablation Electrospray Ionization Time-of-Flight Mass Spectrometry for Direct Analysis of Biological Tissue |
| title_full | Laser Ablation Electrospray Ionization Time-of-Flight Mass Spectrometry for Direct Analysis of Biological Tissue |
| title_fullStr | Laser Ablation Electrospray Ionization Time-of-Flight Mass Spectrometry for Direct Analysis of Biological Tissue |
| title_full_unstemmed | Laser Ablation Electrospray Ionization Time-of-Flight Mass Spectrometry for Direct Analysis of Biological Tissue |
| title_short | Laser Ablation Electrospray Ionization Time-of-Flight Mass Spectrometry for Direct Analysis of Biological Tissue |
| title_sort | laser ablation electrospray ionization time of flight mass spectrometry for direct analysis of biological tissue |
| url | http://dx.doi.org/10.1155/2019/1417035 |
| work_keys_str_mv | AT wenzhaozhou laserablationelectrosprayionizationtimeofflightmassspectrometryfordirectanalysisofbiologicaltissue AT yanhong laserablationelectrosprayionizationtimeofflightmassspectrometryfordirectanalysisofbiologicaltissue AT chaoqunhuang laserablationelectrosprayionizationtimeofflightmassspectrometryfordirectanalysisofbiologicaltissue AT chengyinshen laserablationelectrosprayionizationtimeofflightmassspectrometryfordirectanalysisofbiologicaltissue AT yannanchu laserablationelectrosprayionizationtimeofflightmassspectrometryfordirectanalysisofbiologicaltissue |