Allergen degradation of bee pollen by lactic acid bacteria fermentation and its alleviatory effects on allergic reactions in BALB/c mice
Food allergy as a global health problem threatens food industry. Bee pollen (BP) is a typical food with allergenic potentials, although it performs various nutritional/pharmacological functions to humans. In this study, lactic acid bacteria (LAB) were used to ferment Brassica napus BP for alleviatin...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2024-01-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023001568 |
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| author | Shuting Yin Qiangqiang Li Yuxiao Tao Enning Zhou Kun Wang Wanwen Chen Xiangxin Li Liming Wu |
| author_facet | Shuting Yin Qiangqiang Li Yuxiao Tao Enning Zhou Kun Wang Wanwen Chen Xiangxin Li Liming Wu |
| author_sort | Shuting Yin |
| collection | DOAJ |
| description | Food allergy as a global health problem threatens food industry. Bee pollen (BP) is a typical food with allergenic potentials, although it performs various nutritional/pharmacological functions to humans. In this study, lactic acid bacteria (LAB) were used to ferment Brassica napus BP for alleviating its allergenicity. Four novel allergens (glutaredoxin, oleosin-B2, catalase and lipase) were identified with significant decreases in LAB-fermented BP (FBP) than natural BP by proteomics. Meanwhile, metabolomics analysis showed significant increases of 28 characteristic oligopeptides and amino acids in FBP versus BP, indicating the degradation of LAB on allergens. Moreover, FBP showed alleviatory effects in BALB/c mice, which relieved pathological symptoms and lowered production of allergic mediators. Microbial high-throughput sequencing analysis showed that FBP could regulate gut microbiota and metabolism to strengthen immunity, which were closely correlated with the alleviation of allergic reactivity. These findings could contribute to the development and utilization of hypoallergenic BP products. |
| format | Article |
| id | doaj-art-f2b4d2217ee549c8941261659c882f93 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-f2b4d2217ee549c8941261659c882f932025-02-03T04:09:17ZengTsinghua University PressFood Science and Human Wellness2213-45302024-01-01131349359Allergen degradation of bee pollen by lactic acid bacteria fermentation and its alleviatory effects on allergic reactions in BALB/c miceShuting Yin0Qiangqiang Li1Yuxiao Tao2Enning Zhou3Kun Wang4Wanwen Chen5Xiangxin Li6Liming Wu7Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaInstitute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China; Corresponding author at: Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaInstitute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaInstitute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaDepartment of Animal Science, McGill University, Quebec H9X 3V9, CanadaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaInstitute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaInstitute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China; Corresponding author at: Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, ChinaFood allergy as a global health problem threatens food industry. Bee pollen (BP) is a typical food with allergenic potentials, although it performs various nutritional/pharmacological functions to humans. In this study, lactic acid bacteria (LAB) were used to ferment Brassica napus BP for alleviating its allergenicity. Four novel allergens (glutaredoxin, oleosin-B2, catalase and lipase) were identified with significant decreases in LAB-fermented BP (FBP) than natural BP by proteomics. Meanwhile, metabolomics analysis showed significant increases of 28 characteristic oligopeptides and amino acids in FBP versus BP, indicating the degradation of LAB on allergens. Moreover, FBP showed alleviatory effects in BALB/c mice, which relieved pathological symptoms and lowered production of allergic mediators. Microbial high-throughput sequencing analysis showed that FBP could regulate gut microbiota and metabolism to strengthen immunity, which were closely correlated with the alleviation of allergic reactivity. These findings could contribute to the development and utilization of hypoallergenic BP products.http://www.sciencedirect.com/science/article/pii/S2213453023001568Alleviatory effectsLactic acid bacteria fermentationBee pollen allergenGut microbiotaHost metabolism |
| spellingShingle | Shuting Yin Qiangqiang Li Yuxiao Tao Enning Zhou Kun Wang Wanwen Chen Xiangxin Li Liming Wu Allergen degradation of bee pollen by lactic acid bacteria fermentation and its alleviatory effects on allergic reactions in BALB/c mice Food Science and Human Wellness Alleviatory effects Lactic acid bacteria fermentation Bee pollen allergen Gut microbiota Host metabolism |
| title | Allergen degradation of bee pollen by lactic acid bacteria fermentation and its alleviatory effects on allergic reactions in BALB/c mice |
| title_full | Allergen degradation of bee pollen by lactic acid bacteria fermentation and its alleviatory effects on allergic reactions in BALB/c mice |
| title_fullStr | Allergen degradation of bee pollen by lactic acid bacteria fermentation and its alleviatory effects on allergic reactions in BALB/c mice |
| title_full_unstemmed | Allergen degradation of bee pollen by lactic acid bacteria fermentation and its alleviatory effects on allergic reactions in BALB/c mice |
| title_short | Allergen degradation of bee pollen by lactic acid bacteria fermentation and its alleviatory effects on allergic reactions in BALB/c mice |
| title_sort | allergen degradation of bee pollen by lactic acid bacteria fermentation and its alleviatory effects on allergic reactions in balb c mice |
| topic | Alleviatory effects Lactic acid bacteria fermentation Bee pollen allergen Gut microbiota Host metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023001568 |
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