Intraspecific variability of Puccinia triticina on hexaploid Triticum species and Aegilops trivialis
The analysis of pathogen intraspecific differentiation is a necessary stage for the development of a strategy of wheat leaf rust resistance breeding. To characterize the structure of the populations, virulence to Thatcher lines with leaf rust resistance genes (TcLr) and microsatellite markers may be...
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2017-11-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/1187 |
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| author | E. I. Gultyaeva E. L. Shaydayuk I. A. Kazartsev |
| author_facet | E. I. Gultyaeva E. L. Shaydayuk I. A. Kazartsev |
| author_sort | E. I. Gultyaeva |
| collection | DOAJ |
| description | The analysis of pathogen intraspecific differentiation is a necessary stage for the development of a strategy of wheat leaf rust resistance breeding. To characterize the structure of the populations, virulence to Thatcher lines with leaf rust resistance genes (TcLr) and microsatellite markers may be used. The purpose of the present research is to study the intraspecific variability of the fungus Puccinia triticina Erikss. on Triticum L. hexaploid species and Aegilops trivialis (Zhuk.) Migusch. et Chak. using SSR-markers. Leaves of Triticum aestivum L., T. compactum Host., T. macha Dekapr. et Menabde, T. petropavlovskyi Udacz. et Migusch., T. spelta L., T. sphaerococcum Perc., T. vavilovii Jakubz. and Ae. trivialis with uredinia were collected on the experimental field of the Dagestan experiment station of VIR (Derbent) in 2014. For SSR-analysis 109, monopustule isolates previously characterized for virulence to 20 TcLr-lines were used. As a result of 18 microsatellite loci polymorphism analysis, 16 genotypes were identified. Genetic similarity between collections of isolates from the hexaploid types of wheat was significantly higher based on microsatellite markers than based on virulence. Microsatellite analysis confirmed a high similarity between Derbent isolates of P. triticina coming from the hexaploid Triticum species (including isolates from common wheat) and Aegilops trivialis. |
| format | Article |
| id | doaj-art-f2981454fb24473abb8adf073018e379 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2017-11-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-f2981454fb24473abb8adf073018e3792025-02-01T09:58:04ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592017-11-0121667167610.18699/VJ17.28-o668Intraspecific variability of Puccinia triticina on hexaploid Triticum species and Aegilops trivialisE. I. Gultyaeva0E. L. Shaydayuk1I. A. Kazartsev2All-Russian Institute of Plant Protection, St. Petersburg, PushkinAll-Russian Institute of Plant Protection, St. Petersburg, PushkinAll-Russian Institute of Plant Protection, St. Petersburg, PushkinThe analysis of pathogen intraspecific differentiation is a necessary stage for the development of a strategy of wheat leaf rust resistance breeding. To characterize the structure of the populations, virulence to Thatcher lines with leaf rust resistance genes (TcLr) and microsatellite markers may be used. The purpose of the present research is to study the intraspecific variability of the fungus Puccinia triticina Erikss. on Triticum L. hexaploid species and Aegilops trivialis (Zhuk.) Migusch. et Chak. using SSR-markers. Leaves of Triticum aestivum L., T. compactum Host., T. macha Dekapr. et Menabde, T. petropavlovskyi Udacz. et Migusch., T. spelta L., T. sphaerococcum Perc., T. vavilovii Jakubz. and Ae. trivialis with uredinia were collected on the experimental field of the Dagestan experiment station of VIR (Derbent) in 2014. For SSR-analysis 109, monopustule isolates previously characterized for virulence to 20 TcLr-lines were used. As a result of 18 microsatellite loci polymorphism analysis, 16 genotypes were identified. Genetic similarity between collections of isolates from the hexaploid types of wheat was significantly higher based on microsatellite markers than based on virulence. Microsatellite analysis confirmed a high similarity between Derbent isolates of P. triticina coming from the hexaploid Triticum species (including isolates from common wheat) and Aegilops trivialis.https://vavilov.elpub.ru/jour/article/view/1187genetic structure of populationpuccinia triticinaaegilops trivialistriticum spp.ssr-markers |
| spellingShingle | E. I. Gultyaeva E. L. Shaydayuk I. A. Kazartsev Intraspecific variability of Puccinia triticina on hexaploid Triticum species and Aegilops trivialis Вавиловский журнал генетики и селекции genetic structure of population puccinia triticina aegilops trivialis triticum spp. ssr-markers |
| title | Intraspecific variability of Puccinia triticina on hexaploid Triticum species and Aegilops trivialis |
| title_full | Intraspecific variability of Puccinia triticina on hexaploid Triticum species and Aegilops trivialis |
| title_fullStr | Intraspecific variability of Puccinia triticina on hexaploid Triticum species and Aegilops trivialis |
| title_full_unstemmed | Intraspecific variability of Puccinia triticina on hexaploid Triticum species and Aegilops trivialis |
| title_short | Intraspecific variability of Puccinia triticina on hexaploid Triticum species and Aegilops trivialis |
| title_sort | intraspecific variability of puccinia triticina on hexaploid triticum species and aegilops trivialis |
| topic | genetic structure of population puccinia triticina aegilops trivialis triticum spp. ssr-markers |
| url | https://vavilov.elpub.ru/jour/article/view/1187 |
| work_keys_str_mv | AT eigultyaeva intraspecificvariabilityofpucciniatriticinaonhexaploidtriticumspeciesandaegilopstrivialis AT elshaydayuk intraspecificvariabilityofpucciniatriticinaonhexaploidtriticumspeciesandaegilopstrivialis AT iakazartsev intraspecificvariabilityofpucciniatriticinaonhexaploidtriticumspeciesandaegilopstrivialis |