Comparison of methods for measuring oxygen consumption rate of Hyriopsis cumingii
Oxygen consumption rate is an important physiological indicator for evaluating the environmental adaptation of bivalves. Generally, oxygen consumption rate of bivalve is measured with closed respirometry or flow-through respirometry. So far, the influence of different respirometry on oxygen consumpt...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2013-07-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2012.10.231 |
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| Summary: | Oxygen consumption rate is an important physiological indicator for evaluating the environmental adaptation of bivalves. Generally, oxygen consumption rate of bivalve is measured with closed respirometry or flow-through respirometry. So far, the influence of different respirometry on oxygen consumption rate of bivalve has not been well evaluated.In this paper, we reported the oxygen consumption rate of two-year-old freshwater mussel Hyriopsis cumingii (mussel mass (41.95±6.23) g) measured with closed respirometry, flow-through respirometry and flow-closed respirometry (oxygen consumption rate was measured with closed respirometry after the mussel was acclimated in flow-through respirometer for 2 h), respectively. The measurement of oxygen consumption rate lasted for 2 h, during which water temperature was 22 ℃. After the measurement, water samples were collected at the upper, middle and lower stratifications of the respiration chamber by siphon, and DO (dissolved oxygen) concentrations and pH values were measured. In the respiration chambers of the closed respirometry and flow - closed respirometry, significant stratification in DO concentration was detected.The results showed that the DO concentration was significantly higher at the upper or middle stratification than that at the lower stratification in the respiration chambers of the closed or flow-closed respirometry, whereas no significant DO stratification was detected in the respiration chambers of the flow-through respirometry. The oxygen consumption rates of the mussel based on DO concentrations at the upper and middle stratifications using closed respirometry were (100.9±49.5) and (199.7±49.9) mg/(kg·h) respectively, which were significantly lower than the oxygen consumption rate [(1 710.8±509.6) mg/(kg·h)] calculated based on DO concentration at the lower stratification. The oxygen consumption rates of the mussel based on DO concentrations at the upper and middle stratifications using flow -closed respirometry were (390.06±88.2) and (653.3±78.7) mg/(kg·h) respectively, which were significantly lower than the oxygen consumption rate [(1 116.4±300.9) mg/(kg ·h)] calculated based on DO concentration at the lower stratification. The errors introduced by the DO stratification were calibrated by measuring DO concentrations at different stratifications in the respiration chambers and calculating the oxygen consumption rate with a calibrated equation. The result indicated that the calibrated oxygen consumption rates measured with the closed and flow -closed respirometry were (552.8±115.1) and (625.4±72.4) mg/(kg·h) respectively, which did not statistically differ from the oxygen consumption rate measured with the flow-through respirometry [(504.7±103.1) mg/(kg·h)].The above results reveal that the oxygen consumption rate of the mussel can be erroneously estimated using closed or flow-closed respirometry without the calibration of DO stratification in the respiration chambers. Using the flow-through respirometry can diminish the stress of operation disturbance and monitor the oxygen consumption rate of the tested mussel at a long time scale. Therefore, the flow-through respirometry was recommended for measuring the oxygen consumption rate of bivalve. |
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| ISSN: | 1008-9209 2097-5155 |