Evaluation of 2′-Deoxy-2′-Flouro-5-Methyl-1-β--Arabinofuranosyluracil as a Potential Gene Imaging Agent for Expression In Vivo
2′-Deoxy-2′-flouro-5-methyl-1-β- d -arabinofuranosyluracil (FMAU) has been evaluated in HT-29 cells as a potential positron emission tomography (PET) radiotracer for imaging HSV-tk gene expression in vivo. In vitro experiments demonstrate that the accumulation of [ 14 C]-FMAU in HSV-tk -expressing c...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2002-04-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1162/15353500200202100 |
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| author | Mian M. Alauddin Atranik Shahinian Erlinda M. Gordon Peter S. Conti |
| author_facet | Mian M. Alauddin Atranik Shahinian Erlinda M. Gordon Peter S. Conti |
| author_sort | Mian M. Alauddin |
| collection | DOAJ |
| description | 2′-Deoxy-2′-flouro-5-methyl-1-β- d -arabinofuranosyluracil (FMAU) has been evaluated in HT-29 cells as a potential positron emission tomography (PET) radiotracer for imaging HSV-tk gene expression in vivo. In vitro experiments demonstrate that the accumulation of [ 14 C]-FMAU in HSV-tk -expressing cells is 2.4-fold ( p < .02), 4.0-fold ( p < .001), and 5.3-fold ( p < .001) higher than the wild-type cells at 1, 3, and 5 hr, respectively. In vivo studies revealed that the tumor uptake in HSV-tk -expressing cells was 2.3-fold ( p < .001), 3.0-fold ( p < .001), and 5.5-fold ( p < .001) higher than the control cells at 1, 2, and 5 hr, respectively. FMAU was found to be more sensitive compared to our earlier studies using 9-[(3- 18 F-fluoro-1-hydroxy-2-propoxy)methyl]-guanine ([ 18 F]-FHPG) and 9-(4-[ 18 F]-fluoro-3-hydroxy-methylbutyl)guanine ([ 18 F]-FHBG) in the same cell lines, although, the specificity was less than FHBG. These results suggest that while FMAU labeled with PET isotopes may be useful for imaging HSV-tk -expressing tumors in vivo, multitracer studies across additional tumor models are necessary in order to identify an optimal PET radiotracer. |
| format | Article |
| id | doaj-art-f255ad84326c4aa59ecc9c8ef9d97501 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2002-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-f255ad84326c4aa59ecc9c8ef9d975012025-01-02T22:37:56ZengSAGE PublishingMolecular Imaging1536-01212002-04-01110.1162/1535350020020210010.1162_15353500200202100Evaluation of 2′-Deoxy-2′-Flouro-5-Methyl-1-β--Arabinofuranosyluracil as a Potential Gene Imaging Agent for Expression In VivoMian M. AlauddinAtranik ShahinianErlinda M. GordonPeter S. Conti2′-Deoxy-2′-flouro-5-methyl-1-β- d -arabinofuranosyluracil (FMAU) has been evaluated in HT-29 cells as a potential positron emission tomography (PET) radiotracer for imaging HSV-tk gene expression in vivo. In vitro experiments demonstrate that the accumulation of [ 14 C]-FMAU in HSV-tk -expressing cells is 2.4-fold ( p < .02), 4.0-fold ( p < .001), and 5.3-fold ( p < .001) higher than the wild-type cells at 1, 3, and 5 hr, respectively. In vivo studies revealed that the tumor uptake in HSV-tk -expressing cells was 2.3-fold ( p < .001), 3.0-fold ( p < .001), and 5.5-fold ( p < .001) higher than the control cells at 1, 2, and 5 hr, respectively. FMAU was found to be more sensitive compared to our earlier studies using 9-[(3- 18 F-fluoro-1-hydroxy-2-propoxy)methyl]-guanine ([ 18 F]-FHPG) and 9-(4-[ 18 F]-fluoro-3-hydroxy-methylbutyl)guanine ([ 18 F]-FHBG) in the same cell lines, although, the specificity was less than FHBG. These results suggest that while FMAU labeled with PET isotopes may be useful for imaging HSV-tk -expressing tumors in vivo, multitracer studies across additional tumor models are necessary in order to identify an optimal PET radiotracer.https://doi.org/10.1162/15353500200202100 |
| spellingShingle | Mian M. Alauddin Atranik Shahinian Erlinda M. Gordon Peter S. Conti Evaluation of 2′-Deoxy-2′-Flouro-5-Methyl-1-β--Arabinofuranosyluracil as a Potential Gene Imaging Agent for Expression In Vivo Molecular Imaging |
| title | Evaluation of 2′-Deoxy-2′-Flouro-5-Methyl-1-β--Arabinofuranosyluracil as a Potential Gene Imaging Agent for Expression In Vivo |
| title_full | Evaluation of 2′-Deoxy-2′-Flouro-5-Methyl-1-β--Arabinofuranosyluracil as a Potential Gene Imaging Agent for Expression In Vivo |
| title_fullStr | Evaluation of 2′-Deoxy-2′-Flouro-5-Methyl-1-β--Arabinofuranosyluracil as a Potential Gene Imaging Agent for Expression In Vivo |
| title_full_unstemmed | Evaluation of 2′-Deoxy-2′-Flouro-5-Methyl-1-β--Arabinofuranosyluracil as a Potential Gene Imaging Agent for Expression In Vivo |
| title_short | Evaluation of 2′-Deoxy-2′-Flouro-5-Methyl-1-β--Arabinofuranosyluracil as a Potential Gene Imaging Agent for Expression In Vivo |
| title_sort | evaluation of 2 deoxy 2 flouro 5 methyl 1 β arabinofuranosyluracil as a potential gene imaging agent for expression in vivo |
| url | https://doi.org/10.1162/15353500200202100 |
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