Mesenchymal Stem Cells Combined with Tissue Fusion Technology Promoted Wound Healing in Porcine Bowel Anastomosis
Objective. To evaluate the possible biological effect of allogenic mesenchymal stem cells (MSCs) combined with tissue fusion technology on the anastomosis. Methods. Sixteen pigs were divided into a 7 d group and 14 d group, each of which was further subdivided into an MSC-treated group and a control...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2020/5142797 |
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| _version_ | 1832566515795230720 |
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| author | Hong Pan Ping Keun Lam See W. Tong Kevin K. Leung Anthony Y. Teoh Enders K. Ng |
| author_facet | Hong Pan Ping Keun Lam See W. Tong Kevin K. Leung Anthony Y. Teoh Enders K. Ng |
| author_sort | Hong Pan |
| collection | DOAJ |
| description | Objective. To evaluate the possible biological effect of allogenic mesenchymal stem cells (MSCs) combined with tissue fusion technology on the anastomosis. Methods. Sixteen pigs were divided into a 7 d group and 14 d group, each of which was further subdivided into an MSC-treated group and a control group. Five anastomoses per animal were established using LigaSure ForceTriad (Covidien, MA, USA), a tissue sealing system. Cell migration and tissue-specific differentiation potency, in addition to potential cytokine and genetic changes, were investigated. Results. There were no significant between-group differences in postoperative complications and anastomosis burst pressure. The number of proliferating cell nuclear antigen- (PCNA-) positive cells was significantly higher in the MSC-treated group as compared with that in the control group (P=0.021). Labeled MSCs were found in the mucosal layer, villus, and lamina propria, as well as in the lamina muscularis mucosae, where they exhibited characteristics of smooth muscle cells. Conclusions. Grafted MSCs significantly promoted epithelial and connective cell proliferation and maintained their cell migration capacity and differentiation potential in the fused anastomotic tissues, without causing severe postoperative complications. |
| format | Article |
| id | doaj-art-f2387d8fbc0a45468a01ccaea6ea5da4 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-f2387d8fbc0a45468a01ccaea6ea5da42025-02-03T01:04:06ZengWileyStem Cells International1687-966X1687-96782020-01-01202010.1155/2020/51427975142797Mesenchymal Stem Cells Combined with Tissue Fusion Technology Promoted Wound Healing in Porcine Bowel AnastomosisHong Pan0Ping Keun Lam1See W. Tong2Kevin K. Leung3Anthony Y. Teoh4Enders K. Ng5Department of Surgery, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, ChinaDepartment of Surgery, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, ChinaDepartment of Surgery, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, ChinaDepartment of Surgery, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, ChinaDepartment of Surgery, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, ChinaDepartment of Surgery, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, ChinaObjective. To evaluate the possible biological effect of allogenic mesenchymal stem cells (MSCs) combined with tissue fusion technology on the anastomosis. Methods. Sixteen pigs were divided into a 7 d group and 14 d group, each of which was further subdivided into an MSC-treated group and a control group. Five anastomoses per animal were established using LigaSure ForceTriad (Covidien, MA, USA), a tissue sealing system. Cell migration and tissue-specific differentiation potency, in addition to potential cytokine and genetic changes, were investigated. Results. There were no significant between-group differences in postoperative complications and anastomosis burst pressure. The number of proliferating cell nuclear antigen- (PCNA-) positive cells was significantly higher in the MSC-treated group as compared with that in the control group (P=0.021). Labeled MSCs were found in the mucosal layer, villus, and lamina propria, as well as in the lamina muscularis mucosae, where they exhibited characteristics of smooth muscle cells. Conclusions. Grafted MSCs significantly promoted epithelial and connective cell proliferation and maintained their cell migration capacity and differentiation potential in the fused anastomotic tissues, without causing severe postoperative complications.http://dx.doi.org/10.1155/2020/5142797 |
| spellingShingle | Hong Pan Ping Keun Lam See W. Tong Kevin K. Leung Anthony Y. Teoh Enders K. Ng Mesenchymal Stem Cells Combined with Tissue Fusion Technology Promoted Wound Healing in Porcine Bowel Anastomosis Stem Cells International |
| title | Mesenchymal Stem Cells Combined with Tissue Fusion Technology Promoted Wound Healing in Porcine Bowel Anastomosis |
| title_full | Mesenchymal Stem Cells Combined with Tissue Fusion Technology Promoted Wound Healing in Porcine Bowel Anastomosis |
| title_fullStr | Mesenchymal Stem Cells Combined with Tissue Fusion Technology Promoted Wound Healing in Porcine Bowel Anastomosis |
| title_full_unstemmed | Mesenchymal Stem Cells Combined with Tissue Fusion Technology Promoted Wound Healing in Porcine Bowel Anastomosis |
| title_short | Mesenchymal Stem Cells Combined with Tissue Fusion Technology Promoted Wound Healing in Porcine Bowel Anastomosis |
| title_sort | mesenchymal stem cells combined with tissue fusion technology promoted wound healing in porcine bowel anastomosis |
| url | http://dx.doi.org/10.1155/2020/5142797 |
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