Exercise and Omega-3 Polyunsaturated Fatty Acid Supplementation for the Treatment of Hepatic Steatosis in Hyperphagic OLETF Rats
Background and Aims. This study examined if exercise and omega-3 fatty acid (n3PUFA) supplementation is an effective treatment for hepatic steatosis in obese, hyperphagic Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Methods. Male OLETF rats were divided into 4 groups (n=8/group): (1) remained sed...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Nutrition and Metabolism |
| Online Access: | http://dx.doi.org/10.1155/2012/268680 |
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| author | Sarah J. Borengasser R. Scott Rector Grace M. Uptergrove E. Matthew Morris James W. Perfield Frank W. Booth Kevin L. Fritsche Jamal A. Ibdah John P. Thyfault |
| author_facet | Sarah J. Borengasser R. Scott Rector Grace M. Uptergrove E. Matthew Morris James W. Perfield Frank W. Booth Kevin L. Fritsche Jamal A. Ibdah John P. Thyfault |
| author_sort | Sarah J. Borengasser |
| collection | DOAJ |
| description | Background and Aims. This study examined if exercise and omega-3 fatty acid (n3PUFA) supplementation is an effective treatment for hepatic steatosis in obese, hyperphagic Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Methods. Male OLETF rats were divided into 4 groups (n=8/group): (1) remained sedentary (SED), (2) access to running wheels; (EX) (3) a diet supplemented with 3% of energy from fish oil (n3PUFA-SED); and (4) n3PUFA supplementation plus EX (n3PUFA+EX). The 8 week treatments began at 13 weeks, when hepatic steatosis is present in OLETF-SED rats. Results. EX alone lowered hepatic triglyceride (TAG) while, in contrast, n3PUFAs failed to lower hepatic TAG and blunted the ability of EX to decrease hepatic TAG levels in n3PUFAs+EX. Insulin sensitivity was improved in EX animals, to a lesser extent in n3PUFA+EX rats, and did not differ between n3PUFA-SED and SED rats. Only the EX group displayed higher complete hepatic fatty acid oxidation (FAO) to CO2 and carnitine palmitoyl transferase-1 activity. EX also lowered hepatic fatty acid synthase protein while both EX and n3PUFA+EX decreased stearoyl CoA desaturase-1 protein. Conclusions. Exercise lowers hepatic steatosis through increased complete hepatic FAO, insulin sensitivity, and reduced expression of de novo fatty acid synthesis proteins while n3PUFAs had no effect. |
| format | Article |
| id | doaj-art-f2385f7d1d2f4563b700f9c8e97644fc |
| institution | Kabale University |
| issn | 2090-0724 2090-0732 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nutrition and Metabolism |
| spelling | doaj-art-f2385f7d1d2f4563b700f9c8e97644fc2025-02-03T01:00:01ZengWileyJournal of Nutrition and Metabolism2090-07242090-07322012-01-01201210.1155/2012/268680268680Exercise and Omega-3 Polyunsaturated Fatty Acid Supplementation for the Treatment of Hepatic Steatosis in Hyperphagic OLETF RatsSarah J. Borengasser0R. Scott Rector1Grace M. Uptergrove2E. Matthew Morris3James W. Perfield4Frank W. Booth5Kevin L. Fritsche6Jamal A. Ibdah7John P. Thyfault8Departments of Nutrition and Exercise Physiology, University of Missouri, Columbia, MO 65201, USADepartments of Nutrition and Exercise Physiology, University of Missouri, Columbia, MO 65201, USAInternal Medicine-Division of Gastroenterology, University of Missouri, Columbia, MO 65201, USADepartments of Nutrition and Exercise Physiology, University of Missouri, Columbia, MO 65201, USADepartments of Nutrition and Exercise Physiology, University of Missouri, Columbia, MO 65201, USADepartment of Biomedical Sciences, University of Missouri, Columbia, MO 65201, USADivision of Animal Sciences, University of Missouri, Columbia, MO 65201, USAInternal Medicine-Division of Gastroenterology, University of Missouri, Columbia, MO 65201, USADepartments of Nutrition and Exercise Physiology, University of Missouri, Columbia, MO 65201, USABackground and Aims. This study examined if exercise and omega-3 fatty acid (n3PUFA) supplementation is an effective treatment for hepatic steatosis in obese, hyperphagic Otsuka Long-Evans Tokushima Fatty (OLETF) rats. Methods. Male OLETF rats were divided into 4 groups (n=8/group): (1) remained sedentary (SED), (2) access to running wheels; (EX) (3) a diet supplemented with 3% of energy from fish oil (n3PUFA-SED); and (4) n3PUFA supplementation plus EX (n3PUFA+EX). The 8 week treatments began at 13 weeks, when hepatic steatosis is present in OLETF-SED rats. Results. EX alone lowered hepatic triglyceride (TAG) while, in contrast, n3PUFAs failed to lower hepatic TAG and blunted the ability of EX to decrease hepatic TAG levels in n3PUFAs+EX. Insulin sensitivity was improved in EX animals, to a lesser extent in n3PUFA+EX rats, and did not differ between n3PUFA-SED and SED rats. Only the EX group displayed higher complete hepatic fatty acid oxidation (FAO) to CO2 and carnitine palmitoyl transferase-1 activity. EX also lowered hepatic fatty acid synthase protein while both EX and n3PUFA+EX decreased stearoyl CoA desaturase-1 protein. Conclusions. Exercise lowers hepatic steatosis through increased complete hepatic FAO, insulin sensitivity, and reduced expression of de novo fatty acid synthesis proteins while n3PUFAs had no effect.http://dx.doi.org/10.1155/2012/268680 |
| spellingShingle | Sarah J. Borengasser R. Scott Rector Grace M. Uptergrove E. Matthew Morris James W. Perfield Frank W. Booth Kevin L. Fritsche Jamal A. Ibdah John P. Thyfault Exercise and Omega-3 Polyunsaturated Fatty Acid Supplementation for the Treatment of Hepatic Steatosis in Hyperphagic OLETF Rats Journal of Nutrition and Metabolism |
| title | Exercise and Omega-3 Polyunsaturated Fatty Acid Supplementation for the Treatment of Hepatic Steatosis in Hyperphagic OLETF Rats |
| title_full | Exercise and Omega-3 Polyunsaturated Fatty Acid Supplementation for the Treatment of Hepatic Steatosis in Hyperphagic OLETF Rats |
| title_fullStr | Exercise and Omega-3 Polyunsaturated Fatty Acid Supplementation for the Treatment of Hepatic Steatosis in Hyperphagic OLETF Rats |
| title_full_unstemmed | Exercise and Omega-3 Polyunsaturated Fatty Acid Supplementation for the Treatment of Hepatic Steatosis in Hyperphagic OLETF Rats |
| title_short | Exercise and Omega-3 Polyunsaturated Fatty Acid Supplementation for the Treatment of Hepatic Steatosis in Hyperphagic OLETF Rats |
| title_sort | exercise and omega 3 polyunsaturated fatty acid supplementation for the treatment of hepatic steatosis in hyperphagic oletf rats |
| url | http://dx.doi.org/10.1155/2012/268680 |
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