Protocol for analysis of miRNAs in human melanoma cells
Summary: MicroRNAs (miRNAs) significantly contribute to melanoma plasticity, a key factor in metastasis and therapy resistance. Here, we provide a protocol for analysis of miRNAs in human melanoma cells. We describe two approaches for plating cells, either using cell chambers or attaching cells to m...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725002679 |
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| author | Evelyn Lattmann Tessa Guggiari Patrick Turko Mitchell P. Levesque |
| author_facet | Evelyn Lattmann Tessa Guggiari Patrick Turko Mitchell P. Levesque |
| author_sort | Evelyn Lattmann |
| collection | DOAJ |
| description | Summary: MicroRNAs (miRNAs) significantly contribute to melanoma plasticity, a key factor in metastasis and therapy resistance. Here, we provide a protocol for analysis of miRNAs in human melanoma cells. We describe two approaches for plating cells, either using cell chambers or attaching cells to microscopy slides by means of centrifugation. We first focus on cell pretreatment and staining procedures using the RNAscope Plus small RNA-RNA high-definition (RNAscope Plus smRNA-RNA HD) assay. We then detail the quantitative analysis of miRNA expression and co-localization. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-f22fd2f8b43242f5bdc53bfc7ea513f4 |
| institution | OA Journals |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-f22fd2f8b43242f5bdc53bfc7ea513f42025-08-20T02:00:52ZengElsevierSTAR Protocols2666-16672025-06-016210386110.1016/j.xpro.2025.103861Protocol for analysis of miRNAs in human melanoma cellsEvelyn Lattmann0Tessa Guggiari1Patrick Turko2Mitchell P. Levesque3Department of Dermatology, University of Zurich, University Hospital Zurich, 8952 Schlieren, Switzerland; Corresponding authorDepartment of Dermatology, University of Zurich, University Hospital Zurich, 8952 Schlieren, SwitzerlandDepartment of Dermatology, University of Zurich, University Hospital Zurich, 8952 Schlieren, SwitzerlandDepartment of Dermatology, University of Zurich, University Hospital Zurich, 8952 Schlieren, Switzerland; Corresponding authorSummary: MicroRNAs (miRNAs) significantly contribute to melanoma plasticity, a key factor in metastasis and therapy resistance. Here, we provide a protocol for analysis of miRNAs in human melanoma cells. We describe two approaches for plating cells, either using cell chambers or attaching cells to microscopy slides by means of centrifugation. We first focus on cell pretreatment and staining procedures using the RNAscope Plus small RNA-RNA high-definition (RNAscope Plus smRNA-RNA HD) assay. We then detail the quantitative analysis of miRNA expression and co-localization. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725002679Cell BiologyCell cultureCell-based AssaysCancerMicroscopyIn Situ Hybridization |
| spellingShingle | Evelyn Lattmann Tessa Guggiari Patrick Turko Mitchell P. Levesque Protocol for analysis of miRNAs in human melanoma cells STAR Protocols Cell Biology Cell culture Cell-based Assays Cancer Microscopy In Situ Hybridization |
| title | Protocol for analysis of miRNAs in human melanoma cells |
| title_full | Protocol for analysis of miRNAs in human melanoma cells |
| title_fullStr | Protocol for analysis of miRNAs in human melanoma cells |
| title_full_unstemmed | Protocol for analysis of miRNAs in human melanoma cells |
| title_short | Protocol for analysis of miRNAs in human melanoma cells |
| title_sort | protocol for analysis of mirnas in human melanoma cells |
| topic | Cell Biology Cell culture Cell-based Assays Cancer Microscopy In Situ Hybridization |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725002679 |
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