Multi-component screening coupled with ultrasound-assisted green extraction based on HPLC-HRMS for bio-actives analysis in saffron (Crocus sativus L.)
A database of 115 bio-actives from seven subclasses, including information on retention time, parent ion m/z, fragment m/z, and isotopic fit, was established, and an instrumental method for simultaneous analysis of these targets was optimised. An ultrasound-assisted preparation method at 50 °C for 5...
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Elsevier
2025-01-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157525000835 |
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| author | Shouying Wang Yiqing Song Miaomiao Chen Bing Bai Lin Zhou Changyan Zhou Yongchun Zhang Zhiying Huang Wenshuai Si |
| author_facet | Shouying Wang Yiqing Song Miaomiao Chen Bing Bai Lin Zhou Changyan Zhou Yongchun Zhang Zhiying Huang Wenshuai Si |
| author_sort | Shouying Wang |
| collection | DOAJ |
| description | A database of 115 bio-actives from seven subclasses, including information on retention time, parent ion m/z, fragment m/z, and isotopic fit, was established, and an instrumental method for simultaneous analysis of these targets was optimised. An ultrasound-assisted preparation method at 50 °C for 50 min with a 1:60 solid-liquid ratio, water as the solvent was proposed for the screening of unknown components and the antioxidant analysis in saffron. The self-built database and untargeted analysis identified 32 and 103 bio-actives in saffron, respectively. The comparative analysis revealed that the antioxidant capacity of saffron petals was superior to that of stigmas. Correlation and multivariate statistical analyses indicated that terpenoids may be the main active substances in stigmas, while flavonoids and carboxylic acid derivatives play a pivotal role in conferring antioxidant activity to de-stigmatised saffron. The method serves as an ideal tool for mining the functional components of saffron and other agricultural products. |
| format | Article |
| id | doaj-art-f22c1eb1ad324d6288564f5ae16f6c14 |
| institution | Kabale University |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-f22c1eb1ad324d6288564f5ae16f6c142025-02-12T05:32:41ZengElsevierFood Chemistry: X2590-15752025-01-0125102236Multi-component screening coupled with ultrasound-assisted green extraction based on HPLC-HRMS for bio-actives analysis in saffron (Crocus sativus L.)Shouying Wang0Yiqing Song1Miaomiao Chen2Bing Bai3Lin Zhou4Changyan Zhou5Yongchun Zhang6Zhiying Huang7Wenshuai Si8Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaInstitute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaSchool of Health Sciences and Engineering, Shanghai University of Technology, Shanghai 200093, ChinaInstitute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaForest and Fruit Tree Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaInstitute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaForest and Fruit Tree Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaInstitute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; Corresponding authors at: Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; School of Health Sciences and Engineering, Shanghai University of Technology, Shanghai 200093, China; Corresponding authors at: Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.A database of 115 bio-actives from seven subclasses, including information on retention time, parent ion m/z, fragment m/z, and isotopic fit, was established, and an instrumental method for simultaneous analysis of these targets was optimised. An ultrasound-assisted preparation method at 50 °C for 50 min with a 1:60 solid-liquid ratio, water as the solvent was proposed for the screening of unknown components and the antioxidant analysis in saffron. The self-built database and untargeted analysis identified 32 and 103 bio-actives in saffron, respectively. The comparative analysis revealed that the antioxidant capacity of saffron petals was superior to that of stigmas. Correlation and multivariate statistical analyses indicated that terpenoids may be the main active substances in stigmas, while flavonoids and carboxylic acid derivatives play a pivotal role in conferring antioxidant activity to de-stigmatised saffron. The method serves as an ideal tool for mining the functional components of saffron and other agricultural products.http://www.sciencedirect.com/science/article/pii/S2590157525000835Crocus sativus L., bioactive components, HPLC-HRMS, multivariate statistical analysis, antioxidant |
| spellingShingle | Shouying Wang Yiqing Song Miaomiao Chen Bing Bai Lin Zhou Changyan Zhou Yongchun Zhang Zhiying Huang Wenshuai Si Multi-component screening coupled with ultrasound-assisted green extraction based on HPLC-HRMS for bio-actives analysis in saffron (Crocus sativus L.) Food Chemistry: X Crocus sativus L., bioactive components, HPLC-HRMS, multivariate statistical analysis, antioxidant |
| title | Multi-component screening coupled with ultrasound-assisted green extraction based on HPLC-HRMS for bio-actives analysis in saffron (Crocus sativus L.) |
| title_full | Multi-component screening coupled with ultrasound-assisted green extraction based on HPLC-HRMS for bio-actives analysis in saffron (Crocus sativus L.) |
| title_fullStr | Multi-component screening coupled with ultrasound-assisted green extraction based on HPLC-HRMS for bio-actives analysis in saffron (Crocus sativus L.) |
| title_full_unstemmed | Multi-component screening coupled with ultrasound-assisted green extraction based on HPLC-HRMS for bio-actives analysis in saffron (Crocus sativus L.) |
| title_short | Multi-component screening coupled with ultrasound-assisted green extraction based on HPLC-HRMS for bio-actives analysis in saffron (Crocus sativus L.) |
| title_sort | multi component screening coupled with ultrasound assisted green extraction based on hplc hrms for bio actives analysis in saffron crocus sativus l |
| topic | Crocus sativus L., bioactive components, HPLC-HRMS, multivariate statistical analysis, antioxidant |
| url | http://www.sciencedirect.com/science/article/pii/S2590157525000835 |
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