Effect of Drying Methods on the Phenolic Profile and Antioxidant Capacity of <i>Pithecellobium dulce</i> (Roxb.) Benth. Aril and Its Inhibitory Properties on Human SW480 Colon Adenocarcinoma Cells
<i>Pithecellobium dulce</i> (<i>P. dulce</i>) is a Mexican plant that is consumed raw or in different preparations, and its anti-inflammatory and antioxidant properties have traditionally been useful in treating several conditions. However, the post-harvest drying process can...
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2025-01-01
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| author | Ángel Félix Vargas-Madriz Aarón Kuri-García Ivan Luzardo-Ocampo Roberto Augusto Ferriz-Martínez Teresa García-Gasca Carlos Saldaña Haidel Vargas-Madriz Salvador Horacio Guzmán-Maldonado Jorge Luis Chávez-Servín |
| author_facet | Ángel Félix Vargas-Madriz Aarón Kuri-García Ivan Luzardo-Ocampo Roberto Augusto Ferriz-Martínez Teresa García-Gasca Carlos Saldaña Haidel Vargas-Madriz Salvador Horacio Guzmán-Maldonado Jorge Luis Chávez-Servín |
| author_sort | Ángel Félix Vargas-Madriz |
| collection | DOAJ |
| description | <i>Pithecellobium dulce</i> (<i>P. dulce</i>) is a Mexican plant that is consumed raw or in different preparations, and its anti-inflammatory and antioxidant properties have traditionally been useful in treating several conditions. However, the post-harvest drying process can alter the content of bioactive compounds in <i>P. dulce</i>. This study aims to evaluate the impact of different drying methods on the phenolic profile and antioxidant capacity of this plant, as well as its inhibitory effect on human SW480 colon adenocarcinoma cells. After oven drying, the samples showed a higher amount (<i>p</i> < 0.05) of phenolic compounds, up to 1149.45 ± 69.27 mg GAE/100 g LE, which is 80% more than the freeze-dried samples. Also, the antioxidant capacity was higher in oven-dried samples, with 44.63 ± 2.00 µmol Trolox equivalents/g LE, 108% more than the freeze-dried method. Methanolic extraction, in turn, yielded better results than aqueous and ethanolic extractions. Up to 14 polyphenolic compounds were detected in oven-dried samples. For in vitro assays in SW480 cells, the 50% <i>v</i>/<i>v</i> methanolic extract was used. From this extract, the median lethal concentration (LC<sub>50</sub>) was determined to be 13.76 mg/mL, which represents the concentration necessary to inhibit the growth of half of the cancer cells of this cell line. The extract led to cell cycle arrest in the G1 phase and an increase in apoptosis-induced cell death. The <i>P. dulce</i> extract augmented p53 and decreased KRAS gene expressions. Results suggested pro-apoptotic mechanisms in colon cancer cells in vitro linked to <i>P. dulce</i> bioactive compounds, which are better preserved when oven-dried plants are subjected to methanolic extraction. |
| format | Article |
| id | doaj-art-f20c598633764f969c1b4778c98a7e0d |
| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-f20c598633764f969c1b4778c98a7e0d2025-01-24T13:43:12ZengMDPI AGMolecules1420-30492025-01-0130223310.3390/molecules30020233Effect of Drying Methods on the Phenolic Profile and Antioxidant Capacity of <i>Pithecellobium dulce</i> (Roxb.) Benth. Aril and Its Inhibitory Properties on Human SW480 Colon Adenocarcinoma CellsÁngel Félix Vargas-Madriz0Aarón Kuri-García1Ivan Luzardo-Ocampo2Roberto Augusto Ferriz-Martínez3Teresa García-Gasca4Carlos Saldaña5Haidel Vargas-Madriz6Salvador Horacio Guzmán-Maldonado7Jorge Luis Chávez-Servín8Laboratorio de Biología Celular y Molecular, School of Natural Sciences, Universidad Autonoma de Queretaro, Av. de las Ciencias S/N, Juriquilla, Queretaro 76230, MexicoLaboratorio de Biología Celular y Molecular, School of Natural Sciences, Universidad Autonoma de Queretaro, Av. de las Ciencias S/N, Juriquilla, Queretaro 76230, MexicoInstitute for Obesity Research, Tecnológico de Monterrey, Av. Eugenio Garza Sada 2501, Monterrey 64841, MexicoLaboratorio de Biología Celular y Molecular, School of Natural Sciences, Universidad Autonoma de Queretaro, Av. de las Ciencias S/N, Juriquilla, Queretaro 76230, MexicoLaboratorio de Biología Celular y Molecular, School of Natural Sciences, Universidad Autonoma de Queretaro, Av. de las Ciencias S/N, Juriquilla, Queretaro 76230, MexicoLaboratorio de Biofísica de Membranas y Nanotecnología and Laboratorio Nacional de Visualización Científica Avanzada (LAVIS), School of Natural Sciences, Universidad Autonoma de Queretaro, Av. de las Ciencias S/N, Juriquilla, Queretaro 76230, MexicoDepartamento de Producción Agrícola, Centro Universitario de la Costa Sur, Universidad de Guadalajara—UDG, Av. Independencia Nacional 141, Guadalajara 48900, MexicoLaboratorio de Alimentos, Centro de Investigación Regional del Centro, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Campos Experimental Bajío, Km. 6, Carr. Celaya-San Miguel de Allende, Celaya 38810, MexicoLaboratorio de Biología Celular y Molecular, School of Natural Sciences, Universidad Autonoma de Queretaro, Av. de las Ciencias S/N, Juriquilla, Queretaro 76230, Mexico<i>Pithecellobium dulce</i> (<i>P. dulce</i>) is a Mexican plant that is consumed raw or in different preparations, and its anti-inflammatory and antioxidant properties have traditionally been useful in treating several conditions. However, the post-harvest drying process can alter the content of bioactive compounds in <i>P. dulce</i>. This study aims to evaluate the impact of different drying methods on the phenolic profile and antioxidant capacity of this plant, as well as its inhibitory effect on human SW480 colon adenocarcinoma cells. After oven drying, the samples showed a higher amount (<i>p</i> < 0.05) of phenolic compounds, up to 1149.45 ± 69.27 mg GAE/100 g LE, which is 80% more than the freeze-dried samples. Also, the antioxidant capacity was higher in oven-dried samples, with 44.63 ± 2.00 µmol Trolox equivalents/g LE, 108% more than the freeze-dried method. Methanolic extraction, in turn, yielded better results than aqueous and ethanolic extractions. Up to 14 polyphenolic compounds were detected in oven-dried samples. For in vitro assays in SW480 cells, the 50% <i>v</i>/<i>v</i> methanolic extract was used. From this extract, the median lethal concentration (LC<sub>50</sub>) was determined to be 13.76 mg/mL, which represents the concentration necessary to inhibit the growth of half of the cancer cells of this cell line. The extract led to cell cycle arrest in the G1 phase and an increase in apoptosis-induced cell death. The <i>P. dulce</i> extract augmented p53 and decreased KRAS gene expressions. Results suggested pro-apoptotic mechanisms in colon cancer cells in vitro linked to <i>P. dulce</i> bioactive compounds, which are better preserved when oven-dried plants are subjected to methanolic extraction.https://www.mdpi.com/1420-3049/30/2/233<i>Pithecellobium dulce</i>anti-cancerantioxidantdrying methodsphenolic compounds |
| spellingShingle | Ángel Félix Vargas-Madriz Aarón Kuri-García Ivan Luzardo-Ocampo Roberto Augusto Ferriz-Martínez Teresa García-Gasca Carlos Saldaña Haidel Vargas-Madriz Salvador Horacio Guzmán-Maldonado Jorge Luis Chávez-Servín Effect of Drying Methods on the Phenolic Profile and Antioxidant Capacity of <i>Pithecellobium dulce</i> (Roxb.) Benth. Aril and Its Inhibitory Properties on Human SW480 Colon Adenocarcinoma Cells Molecules <i>Pithecellobium dulce</i> anti-cancer antioxidant drying methods phenolic compounds |
| title | Effect of Drying Methods on the Phenolic Profile and Antioxidant Capacity of <i>Pithecellobium dulce</i> (Roxb.) Benth. Aril and Its Inhibitory Properties on Human SW480 Colon Adenocarcinoma Cells |
| title_full | Effect of Drying Methods on the Phenolic Profile and Antioxidant Capacity of <i>Pithecellobium dulce</i> (Roxb.) Benth. Aril and Its Inhibitory Properties on Human SW480 Colon Adenocarcinoma Cells |
| title_fullStr | Effect of Drying Methods on the Phenolic Profile and Antioxidant Capacity of <i>Pithecellobium dulce</i> (Roxb.) Benth. Aril and Its Inhibitory Properties on Human SW480 Colon Adenocarcinoma Cells |
| title_full_unstemmed | Effect of Drying Methods on the Phenolic Profile and Antioxidant Capacity of <i>Pithecellobium dulce</i> (Roxb.) Benth. Aril and Its Inhibitory Properties on Human SW480 Colon Adenocarcinoma Cells |
| title_short | Effect of Drying Methods on the Phenolic Profile and Antioxidant Capacity of <i>Pithecellobium dulce</i> (Roxb.) Benth. Aril and Its Inhibitory Properties on Human SW480 Colon Adenocarcinoma Cells |
| title_sort | effect of drying methods on the phenolic profile and antioxidant capacity of i pithecellobium dulce i roxb benth aril and its inhibitory properties on human sw480 colon adenocarcinoma cells |
| topic | <i>Pithecellobium dulce</i> anti-cancer antioxidant drying methods phenolic compounds |
| url | https://www.mdpi.com/1420-3049/30/2/233 |
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