CAV1 promotes epithelial-to-mesenchymal transition (EMT) and chronic renal allograft interstitial fibrosis by activating the ferroptosis pathway

BackgroundChronic allograft dysfunction (CAD) stands as a critical factor that limits the long-term viability of transplanted kidneys. Ferroptosis is an iron-dependent form of programmed cell death increasingly linked to chronic fibrosis. However, the mechanism by which ferroptosis contributes to th...

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Main Authors: Qianguang Han, Bin Ni, Wei Bao, Junqi Zhang, Ming Zheng, Jinxu Miu, Zijie Wang, Jingwen Yuan, Jun Tao, Zhijian Han, Min Gu, Xiaobing Ju, Ruoyun Tan
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-02-01
Series:Frontiers in Immunology
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Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2025.1523855/full
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author Qianguang Han
Bin Ni
Wei Bao
Junqi Zhang
Ming Zheng
Jinxu Miu
Zijie Wang
Jingwen Yuan
Jun Tao
Zhijian Han
Min Gu
Xiaobing Ju
Ruoyun Tan
author_facet Qianguang Han
Bin Ni
Wei Bao
Junqi Zhang
Ming Zheng
Jinxu Miu
Zijie Wang
Jingwen Yuan
Jun Tao
Zhijian Han
Min Gu
Xiaobing Ju
Ruoyun Tan
author_sort Qianguang Han
collection DOAJ
description BackgroundChronic allograft dysfunction (CAD) stands as a critical factor that limits the long-term viability of transplanted kidneys. Ferroptosis is an iron-dependent form of programmed cell death increasingly linked to chronic fibrosis. However, the mechanism by which ferroptosis contributes to the onset and progression of CAD remains unclear.MethodsThis study analyzed transcriptome data from renal transplant biopsy samples in the Gene Expression Omnibus (GEO), through clinical samples, animal models, and cell experiments, this study investigated the mechanism by which Caveolin-1 (CAV1) promotes CAD through the regulation of the ferroptosis pathway.ResultsThe elevated levels of CAV1 were found to positively correlate with CAD incidence. Clinical and animal model validation confirmed heightened CAV1 expression in CAD. In vitro experiments demonstrated that CAV1 can directly promote chronic renal allograft interstitial fibrosis by regulating ferroptosis in renal tubular epithelial cells; additionally, it can promote epithelial-to-mesenchymal transition (EMT) by secreting Interleukin- 6 (IL-6), thereby further contributing to CAD.ConclusionCAV1 plays a critical role in the development of CAD by promoting EMT and chronic renal allograft interstitial fibrosis through the ferroptosis pathway. Adjusting ferroptosis by altering the expression abundance of CAV1 may become an important method for the prevention and treatment of CAD in the future.
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institution Kabale University
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spelling doaj-art-f201ea63bf184367b43b2f290702ce752025-02-12T07:25:56ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-02-011610.3389/fimmu.2025.15238551523855CAV1 promotes epithelial-to-mesenchymal transition (EMT) and chronic renal allograft interstitial fibrosis by activating the ferroptosis pathwayQianguang Han0Bin Ni1Wei Bao2Junqi Zhang3Ming Zheng4Jinxu Miu5Zijie Wang6Jingwen Yuan7Jun Tao8Zhijian Han9Min Gu10Xiaobing Ju11Ruoyun Tan12Department of Urology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, ChinaDepartment of Urology, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, ChinaDepartment of Urology, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, ChinaDepartment of Urology, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, ChinaDepartment of Urology, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, ChinaDepartment of Urology, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, ChinaDepartment of Urology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, ChinaDepartment of Urology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, ChinaDepartment of Urology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, ChinaDepartment of Urology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, ChinaDepartment of Urology, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, ChinaDepartment of Urology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, ChinaDepartment of Urology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, ChinaBackgroundChronic allograft dysfunction (CAD) stands as a critical factor that limits the long-term viability of transplanted kidneys. Ferroptosis is an iron-dependent form of programmed cell death increasingly linked to chronic fibrosis. However, the mechanism by which ferroptosis contributes to the onset and progression of CAD remains unclear.MethodsThis study analyzed transcriptome data from renal transplant biopsy samples in the Gene Expression Omnibus (GEO), through clinical samples, animal models, and cell experiments, this study investigated the mechanism by which Caveolin-1 (CAV1) promotes CAD through the regulation of the ferroptosis pathway.ResultsThe elevated levels of CAV1 were found to positively correlate with CAD incidence. Clinical and animal model validation confirmed heightened CAV1 expression in CAD. In vitro experiments demonstrated that CAV1 can directly promote chronic renal allograft interstitial fibrosis by regulating ferroptosis in renal tubular epithelial cells; additionally, it can promote epithelial-to-mesenchymal transition (EMT) by secreting Interleukin- 6 (IL-6), thereby further contributing to CAD.ConclusionCAV1 plays a critical role in the development of CAD by promoting EMT and chronic renal allograft interstitial fibrosis through the ferroptosis pathway. Adjusting ferroptosis by altering the expression abundance of CAV1 may become an important method for the prevention and treatment of CAD in the future.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1523855/fullchronic renal graft dysfunction (CAD)epithelial-mesenchymal transition (EMT)ferroptosisCaveolin-1(CAV1)Interleukin-6 (IL-6)
spellingShingle Qianguang Han
Bin Ni
Wei Bao
Junqi Zhang
Ming Zheng
Jinxu Miu
Zijie Wang
Jingwen Yuan
Jun Tao
Zhijian Han
Min Gu
Xiaobing Ju
Ruoyun Tan
CAV1 promotes epithelial-to-mesenchymal transition (EMT) and chronic renal allograft interstitial fibrosis by activating the ferroptosis pathway
Frontiers in Immunology
chronic renal graft dysfunction (CAD)
epithelial-mesenchymal transition (EMT)
ferroptosis
Caveolin-1(CAV1)
Interleukin-6 (IL-6)
title CAV1 promotes epithelial-to-mesenchymal transition (EMT) and chronic renal allograft interstitial fibrosis by activating the ferroptosis pathway
title_full CAV1 promotes epithelial-to-mesenchymal transition (EMT) and chronic renal allograft interstitial fibrosis by activating the ferroptosis pathway
title_fullStr CAV1 promotes epithelial-to-mesenchymal transition (EMT) and chronic renal allograft interstitial fibrosis by activating the ferroptosis pathway
title_full_unstemmed CAV1 promotes epithelial-to-mesenchymal transition (EMT) and chronic renal allograft interstitial fibrosis by activating the ferroptosis pathway
title_short CAV1 promotes epithelial-to-mesenchymal transition (EMT) and chronic renal allograft interstitial fibrosis by activating the ferroptosis pathway
title_sort cav1 promotes epithelial to mesenchymal transition emt and chronic renal allograft interstitial fibrosis by activating the ferroptosis pathway
topic chronic renal graft dysfunction (CAD)
epithelial-mesenchymal transition (EMT)
ferroptosis
Caveolin-1(CAV1)
Interleukin-6 (IL-6)
url https://www.frontiersin.org/articles/10.3389/fimmu.2025.1523855/full
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