Immunological efficiency of Haemophilus influenzae type b polyribosyl ribitol phosphate combined with detoxified lipooligosaccharide in a rabbit model
Background and Objectives: Haemophilus influenzae type b (Hib) could cause severe life-threatening infections in children. Combine vaccines have reduced invasive diseases, but disease management is still necessary. The aim of this research was to evaluate the immunological efficiency of polyribosyl...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2025-02-01
|
| Series: | Iranian Journal of Microbiology |
| Subjects: | |
| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/3250 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823863964855959552 |
|---|---|
| author | Amin Arsang Seyed Davar Siadat Masoumeh Saberpour Farshad Nojoomi |
| author_facet | Amin Arsang Seyed Davar Siadat Masoumeh Saberpour Farshad Nojoomi |
| author_sort | Amin Arsang |
| collection | DOAJ |
| description |
Background and Objectives: Haemophilus influenzae type b (Hib) could cause severe life-threatening infections in children. Combine vaccines have reduced invasive diseases, but disease management is still necessary. The aim of this research was to evaluate the immunological efficiency of polyribosyl-ribitol-phosphate combined with detoxified lipooligosaccharide (PRP-dLOS) in a rabbit model.
Materials and Methods: PRP purification, LOS extraction, and endotoxin evaluation were performed using modified CY medium, hot phenol, and limulus amebocyte lysate methods, respectively. Rabbit groups were immunized with PRP (10 µg), dLOS (20 µg), and PRP-dLOS combine (10 µg+20 µg) three times on days 0, 14, and 28. Serum samples were acquired on days 0, 14, and 28 post-immunization, then IgM and IgG levels were assayed by enzyme-linked immunosorbent assay.
Results: The concentrations of PRP, dLOS, and endotoxin were 1160 mg/L, 440 μg/mL, and 1450 EU/mL, respectively. PRP-dLOS combine led to a significant increase in IgG and IgM levels on days 14 and 28 post-immunization. After immunization with PRP-dLOS combine, serum levels of IgM and IgG increased from 16.8 to 29.3 µg/mL and 29.8 to 61.4 µg/mL, respectively from day 14 to day 28.
Conclusion: PRP-dLOS combine is a promising approach for Hib management without the fear of delay in immune responses and interference with other vaccines.
|
| format | Article |
| id | doaj-art-f1ddb25b8b084b00907e54061f506a23 |
| institution | Kabale University |
| issn | 2008-3289 2008-4447 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj-art-f1ddb25b8b084b00907e54061f506a232025-02-09T09:01:27ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472025-02-01171Immunological efficiency of Haemophilus influenzae type b polyribosyl ribitol phosphate combined with detoxified lipooligosaccharide in a rabbit modelAmin Arsang0Seyed Davar Siadat1Masoumeh Saberpour2Farshad Nojoomi3Department of Mycobacteriology & Pulmonary Research, Pasteur Institute of Iran, Tehran, IranDepartment of Mycobacteriology & Pulmonary Research, Pasteur Institute of Iran, Tehran, IranDepartment of Influenza and Other Respiratory Viruses, Pasteur Institute of Iran, Tehran, IranInfectious Diseases Research Center, AJA University of Medical Sciences, Tehran, Iran Background and Objectives: Haemophilus influenzae type b (Hib) could cause severe life-threatening infections in children. Combine vaccines have reduced invasive diseases, but disease management is still necessary. The aim of this research was to evaluate the immunological efficiency of polyribosyl-ribitol-phosphate combined with detoxified lipooligosaccharide (PRP-dLOS) in a rabbit model. Materials and Methods: PRP purification, LOS extraction, and endotoxin evaluation were performed using modified CY medium, hot phenol, and limulus amebocyte lysate methods, respectively. Rabbit groups were immunized with PRP (10 µg), dLOS (20 µg), and PRP-dLOS combine (10 µg+20 µg) three times on days 0, 14, and 28. Serum samples were acquired on days 0, 14, and 28 post-immunization, then IgM and IgG levels were assayed by enzyme-linked immunosorbent assay. Results: The concentrations of PRP, dLOS, and endotoxin were 1160 mg/L, 440 μg/mL, and 1450 EU/mL, respectively. PRP-dLOS combine led to a significant increase in IgG and IgM levels on days 14 and 28 post-immunization. After immunization with PRP-dLOS combine, serum levels of IgM and IgG increased from 16.8 to 29.3 µg/mL and 29.8 to 61.4 µg/mL, respectively from day 14 to day 28. Conclusion: PRP-dLOS combine is a promising approach for Hib management without the fear of delay in immune responses and interference with other vaccines. https://ijm.tums.ac.ir/index.php/ijm/article/view/3250Haemophilus influenzae type b;Immunological;Lipooligosaccharide;Meningitis |
| spellingShingle | Amin Arsang Seyed Davar Siadat Masoumeh Saberpour Farshad Nojoomi Immunological efficiency of Haemophilus influenzae type b polyribosyl ribitol phosphate combined with detoxified lipooligosaccharide in a rabbit model Iranian Journal of Microbiology Haemophilus influenzae type b; Immunological; Lipooligosaccharide; Meningitis |
| title | Immunological efficiency of Haemophilus influenzae type b polyribosyl ribitol phosphate combined with detoxified lipooligosaccharide in a rabbit model |
| title_full | Immunological efficiency of Haemophilus influenzae type b polyribosyl ribitol phosphate combined with detoxified lipooligosaccharide in a rabbit model |
| title_fullStr | Immunological efficiency of Haemophilus influenzae type b polyribosyl ribitol phosphate combined with detoxified lipooligosaccharide in a rabbit model |
| title_full_unstemmed | Immunological efficiency of Haemophilus influenzae type b polyribosyl ribitol phosphate combined with detoxified lipooligosaccharide in a rabbit model |
| title_short | Immunological efficiency of Haemophilus influenzae type b polyribosyl ribitol phosphate combined with detoxified lipooligosaccharide in a rabbit model |
| title_sort | immunological efficiency of haemophilus influenzae type b polyribosyl ribitol phosphate combined with detoxified lipooligosaccharide in a rabbit model |
| topic | Haemophilus influenzae type b; Immunological; Lipooligosaccharide; Meningitis |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/3250 |
| work_keys_str_mv | AT aminarsang immunologicalefficiencyofhaemophilusinfluenzaetypebpolyribosylribitolphosphatecombinedwithdetoxifiedlipooligosaccharideinarabbitmodel AT seyeddavarsiadat immunologicalefficiencyofhaemophilusinfluenzaetypebpolyribosylribitolphosphatecombinedwithdetoxifiedlipooligosaccharideinarabbitmodel AT masoumehsaberpour immunologicalefficiencyofhaemophilusinfluenzaetypebpolyribosylribitolphosphatecombinedwithdetoxifiedlipooligosaccharideinarabbitmodel AT farshadnojoomi immunologicalefficiencyofhaemophilusinfluenzaetypebpolyribosylribitolphosphatecombinedwithdetoxifiedlipooligosaccharideinarabbitmodel |