Application of HPLC Combined with Laser Induced Fluorescence for Protein Profile Analysis of Tissue Homogenates in Cervical Cancer
A highly objective method, High Performance Liquid Chromatography with Laser Induced Fluorescence (HPLC-LIF) technique was used to study the protein profiles of normal and cervical cancer tissue homogenates. A total of 44 samples including normal cervical biopsy samples from the hysterectomy patien...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/976421 |
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| author | Sujatha Bhat Ajeetkumar Patil Lavanya Rai V. B. Kartha Santhosh Chidangil |
| author_facet | Sujatha Bhat Ajeetkumar Patil Lavanya Rai V. B. Kartha Santhosh Chidangil |
| author_sort | Sujatha Bhat |
| collection | DOAJ |
| description | A highly objective method, High Performance Liquid Chromatography with Laser Induced Fluorescence (HPLC-LIF) technique was used to study the protein profiles of normal and cervical cancer tissue homogenates. A total of 44 samples including normal cervical biopsy samples from the hysterectomy patients and the patients suffering from different stages of the cervical cancer were recorded by HPLC-LIF and analysed by Principle Component Analysis (PCA) to get statistical information on different tissue components. Discrimination of different stages of the samples was carried out by considering three parameters—scores of factor, spectral residual, and Mahalanobis Distance. Diagnostic accuracy of the method was evaluated using Receiver Operating Characteristic (ROC) analysis, and Youden's index (J) plots. The PCA results showed high sensitivity and specificity (∼100) for cervical cancer diagnosis. ROC and Youden's index curves for both normal and malignant standard sets show good diagnostic accuracy with high AUC values. The statistical analysis has shown that the differences in protein profiles can be used to diagnose biochemical changes in the tissue, and thus can be readily applied for the detection of cervical cancer, even in situations where a histopathology examination is not easy because of nonavailability of experienced pathologists. |
| format | Article |
| id | doaj-art-f1ab0e879cdc41adaf1cdcf92a31391e |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-f1ab0e879cdc41adaf1cdcf92a31391e2025-02-03T01:31:27ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/976421976421Application of HPLC Combined with Laser Induced Fluorescence for Protein Profile Analysis of Tissue Homogenates in Cervical CancerSujatha Bhat0Ajeetkumar Patil1Lavanya Rai2V. B. Kartha3Santhosh Chidangil4KMC International Center, Manipal University, Manipal 576104, IndiaCentre for Atomic and Molecular Physics, Manipal University, Karnataka, Manipal 576104, IndiaDepartment of Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal 576104, IndiaCentre for Atomic and Molecular Physics, Manipal University, Karnataka, Manipal 576104, IndiaCentre for Atomic and Molecular Physics, Manipal University, Karnataka, Manipal 576104, IndiaA highly objective method, High Performance Liquid Chromatography with Laser Induced Fluorescence (HPLC-LIF) technique was used to study the protein profiles of normal and cervical cancer tissue homogenates. A total of 44 samples including normal cervical biopsy samples from the hysterectomy patients and the patients suffering from different stages of the cervical cancer were recorded by HPLC-LIF and analysed by Principle Component Analysis (PCA) to get statistical information on different tissue components. Discrimination of different stages of the samples was carried out by considering three parameters—scores of factor, spectral residual, and Mahalanobis Distance. Diagnostic accuracy of the method was evaluated using Receiver Operating Characteristic (ROC) analysis, and Youden's index (J) plots. The PCA results showed high sensitivity and specificity (∼100) for cervical cancer diagnosis. ROC and Youden's index curves for both normal and malignant standard sets show good diagnostic accuracy with high AUC values. The statistical analysis has shown that the differences in protein profiles can be used to diagnose biochemical changes in the tissue, and thus can be readily applied for the detection of cervical cancer, even in situations where a histopathology examination is not easy because of nonavailability of experienced pathologists.http://dx.doi.org/10.1100/2012/976421 |
| spellingShingle | Sujatha Bhat Ajeetkumar Patil Lavanya Rai V. B. Kartha Santhosh Chidangil Application of HPLC Combined with Laser Induced Fluorescence for Protein Profile Analysis of Tissue Homogenates in Cervical Cancer The Scientific World Journal |
| title | Application of HPLC Combined with Laser Induced Fluorescence for Protein Profile Analysis of Tissue Homogenates in Cervical Cancer |
| title_full | Application of HPLC Combined with Laser Induced Fluorescence for Protein Profile Analysis of Tissue Homogenates in Cervical Cancer |
| title_fullStr | Application of HPLC Combined with Laser Induced Fluorescence for Protein Profile Analysis of Tissue Homogenates in Cervical Cancer |
| title_full_unstemmed | Application of HPLC Combined with Laser Induced Fluorescence for Protein Profile Analysis of Tissue Homogenates in Cervical Cancer |
| title_short | Application of HPLC Combined with Laser Induced Fluorescence for Protein Profile Analysis of Tissue Homogenates in Cervical Cancer |
| title_sort | application of hplc combined with laser induced fluorescence for protein profile analysis of tissue homogenates in cervical cancer |
| url | http://dx.doi.org/10.1100/2012/976421 |
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