A novel roseosiphovirus infecting dinoroseobacter shibae DFL12T represents a new genus
Abstract Bacteria belonging to the Roseobacter clade are key players in marine ecosystems, contributing significantly to carbon and sulfur cycles. Marine viruses, particularly those targeting Roseobacter, play crucial roles in regulating microbial communities and biogeochemical processes. Despite th...
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BMC
2025-02-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-025-11274-w |
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| author | Nana Wei Longfei Lu Yingying Li Bo Ding Lanlan Cai Yunlan Yang |
| author_facet | Nana Wei Longfei Lu Yingying Li Bo Ding Lanlan Cai Yunlan Yang |
| author_sort | Nana Wei |
| collection | DOAJ |
| description | Abstract Bacteria belonging to the Roseobacter clade are key players in marine ecosystems, contributing significantly to carbon and sulfur cycles. Marine viruses, particularly those targeting Roseobacter, play crucial roles in regulating microbial communities and biogeochemical processes. Despite their importance, phages infecting organisms of the Roseobacter clade remain poorly understood. In this study, a novel roseophage, vB_DshS-R26L (R26L), infecting Dinoroseobacter shibae DFL12T, was isolated and characterized in terms of physiological and genomic properties. R26L has siphovirus morphology with an elongated head and a long, non-flexible tail. The phage has a narrow host range and demonstrates a long infection cycle with a latent period of 3.5 h and a burst size of 22 plaque-forming units (PFU cell− 1). R26L possesses a circular, double-stranded DNA genome of 79,534 bp with a G + C content of 62.6%, encoding a total of 116 open reading frames. Notably, seven auxiliary metabolic genes (AMGs), including those related to phosphate metabolism and queuosine biosynthesis, were identified. Phylogenetic and taxonomic analyses revealed that R26L represents a new genus, with its highest intergenomic similarities being 54.7% to another roseophage (R5C). By elucidating the unique characteristics of R26L, this study highlights the complexity of phage infections and the genomic diversity of roseophages, offering valuable insights into the ecological significance of Roseobacter–phage interactions in marine environments. |
| format | Article |
| id | doaj-art-f16cc520a2cc495a97524912c900443b |
| institution | Kabale University |
| issn | 1471-2164 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj-art-f16cc520a2cc495a97524912c900443b2025-02-09T12:13:59ZengBMCBMC Genomics1471-21642025-02-0126111010.1186/s12864-025-11274-wA novel roseosiphovirus infecting dinoroseobacter shibae DFL12T represents a new genusNana Wei0Longfei Lu1Yingying Li2Bo Ding3Lanlan Cai4Yunlan Yang5State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen UniversityKey Laboratory of Tropical Marine Ecosystem and Bioresource, Fourth Institute of Oceanography, Ministry of Natural ResourcesState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen UniversityState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen UniversityEarth, Ocean and Atmospheric Sciences Thrust, The Hong Kong University of Science and Technology (Guangzhou)Archaeal Biology Center, Synthetic Biology Research Center, Shenzhen Key Laboratory of Marine Microbiome Engineering, Key Laboratory of Marine Microbiome Engineering of Guangdong Higher Education Institutes, Institute for Advanced Study, Shenzhen UniversityAbstract Bacteria belonging to the Roseobacter clade are key players in marine ecosystems, contributing significantly to carbon and sulfur cycles. Marine viruses, particularly those targeting Roseobacter, play crucial roles in regulating microbial communities and biogeochemical processes. Despite their importance, phages infecting organisms of the Roseobacter clade remain poorly understood. In this study, a novel roseophage, vB_DshS-R26L (R26L), infecting Dinoroseobacter shibae DFL12T, was isolated and characterized in terms of physiological and genomic properties. R26L has siphovirus morphology with an elongated head and a long, non-flexible tail. The phage has a narrow host range and demonstrates a long infection cycle with a latent period of 3.5 h and a burst size of 22 plaque-forming units (PFU cell− 1). R26L possesses a circular, double-stranded DNA genome of 79,534 bp with a G + C content of 62.6%, encoding a total of 116 open reading frames. Notably, seven auxiliary metabolic genes (AMGs), including those related to phosphate metabolism and queuosine biosynthesis, were identified. Phylogenetic and taxonomic analyses revealed that R26L represents a new genus, with its highest intergenomic similarities being 54.7% to another roseophage (R5C). By elucidating the unique characteristics of R26L, this study highlights the complexity of phage infections and the genomic diversity of roseophages, offering valuable insights into the ecological significance of Roseobacter–phage interactions in marine environments.https://doi.org/10.1186/s12864-025-11274-wRoseophageBiological featuresGenomic analysisTaxonomy |
| spellingShingle | Nana Wei Longfei Lu Yingying Li Bo Ding Lanlan Cai Yunlan Yang A novel roseosiphovirus infecting dinoroseobacter shibae DFL12T represents a new genus BMC Genomics Roseophage Biological features Genomic analysis Taxonomy |
| title | A novel roseosiphovirus infecting dinoroseobacter shibae DFL12T represents a new genus |
| title_full | A novel roseosiphovirus infecting dinoroseobacter shibae DFL12T represents a new genus |
| title_fullStr | A novel roseosiphovirus infecting dinoroseobacter shibae DFL12T represents a new genus |
| title_full_unstemmed | A novel roseosiphovirus infecting dinoroseobacter shibae DFL12T represents a new genus |
| title_short | A novel roseosiphovirus infecting dinoroseobacter shibae DFL12T represents a new genus |
| title_sort | novel roseosiphovirus infecting dinoroseobacter shibae dfl12t represents a new genus |
| topic | Roseophage Biological features Genomic analysis Taxonomy |
| url | https://doi.org/10.1186/s12864-025-11274-w |
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