Micro-channels array device fabricated via two photon lithography for cell migration studies in Neuroblastoma metastatic disseminationAll original data are available at: .
Detailed studies of cells migration are key in understanding tumors metastatic spread. We used two-photon polymerization (2PP) to create precise microdevices for studying cell migration through micro-channels at a single cell resolution. Micro-channels are designed to mimic the structure of lymphati...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | OpenNano |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352952025000027 |
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| author | Sara Micheli Caterina Piunti Elisa Varaschin Marianna Peditto Maria Luz Suarez Marco Sorgato Elisa Cimetta |
| author_facet | Sara Micheli Caterina Piunti Elisa Varaschin Marianna Peditto Maria Luz Suarez Marco Sorgato Elisa Cimetta |
| author_sort | Sara Micheli |
| collection | DOAJ |
| description | Detailed studies of cells migration are key in understanding tumors metastatic spread. We used two-photon polymerization (2PP) to create precise microdevices for studying cell migration through micro-channels at a single cell resolution. Micro-channels are designed to mimic the structure of lymphatic vessels, conduits for cell movement in vivo. Neuroblastoma (NB) and human Mesenchymal Stem Cells (MSCs) represent the main tumor and its primary metastatic site. Our results revealed distinctive behaviors of NB and MSCs, both individually and in co-culture, hinting at a tumor-suppressive role of MSCs inhibiting NB migration. Pre-exposure of MSCs to NB-derived extracellular vesicles (EVs) significantly increased their motility towards tumor cells. Our platform more effectively replicates the in vivo environment of metastatic migration, with results providing new insights into the early dissemination of NB. Such microdevices hold great promise for advancing our understanding of metastasis and aiding the development of targeted anti-cancer therapies. |
| format | Article |
| id | doaj-art-f167907f8f434b509e9bdcab6f5f5667 |
| institution | Kabale University |
| issn | 2352-9520 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | OpenNano |
| spelling | doaj-art-f167907f8f434b509e9bdcab6f5f56672025-01-18T05:04:51ZengElsevierOpenNano2352-95202025-03-0122100233Micro-channels array device fabricated via two photon lithography for cell migration studies in Neuroblastoma metastatic disseminationAll original data are available at: .Sara Micheli0Caterina Piunti1Elisa Varaschin2Marianna Peditto3Maria Luz Suarez4Marco Sorgato5Elisa Cimetta6Department of Industrial Engineering (DII), University of Padua, Padova 35131, Italy; Fondazione Istituto di Ricerca Pediatrica Città della Speranza (IRP), Corso Stati Uniti 4, Padova 35127, ItalyDepartment of Industrial Engineering (DII), University of Padua, Padova 35131, Italy; Fondazione Istituto di Ricerca Pediatrica Città della Speranza (IRP), Corso Stati Uniti 4, Padova 35127, ItalyDepartment of Industrial Engineering (DII), University of Padua, Padova 35131, ItalyDepartment of Industrial Engineering (DII), University of Padua, Padova 35131, ItalyDepartment of Industrial Engineering (DII), University of Padua, Padova 35131, ItalyDepartment of Industrial Engineering (DII), University of Padua, Padova 35131, ItalyDepartment of Industrial Engineering (DII), University of Padua, Padova 35131, Italy; Fondazione Istituto di Ricerca Pediatrica Città della Speranza (IRP), Corso Stati Uniti 4, Padova 35127, Italy; Corresponding author.Detailed studies of cells migration are key in understanding tumors metastatic spread. We used two-photon polymerization (2PP) to create precise microdevices for studying cell migration through micro-channels at a single cell resolution. Micro-channels are designed to mimic the structure of lymphatic vessels, conduits for cell movement in vivo. Neuroblastoma (NB) and human Mesenchymal Stem Cells (MSCs) represent the main tumor and its primary metastatic site. Our results revealed distinctive behaviors of NB and MSCs, both individually and in co-culture, hinting at a tumor-suppressive role of MSCs inhibiting NB migration. Pre-exposure of MSCs to NB-derived extracellular vesicles (EVs) significantly increased their motility towards tumor cells. Our platform more effectively replicates the in vivo environment of metastatic migration, with results providing new insights into the early dissemination of NB. Such microdevices hold great promise for advancing our understanding of metastasis and aiding the development of targeted anti-cancer therapies.http://www.sciencedirect.com/science/article/pii/S2352952025000027Cancer cell motilityMetastasisMicroscale devicesTwo-photon polymerization (2PP) |
| spellingShingle | Sara Micheli Caterina Piunti Elisa Varaschin Marianna Peditto Maria Luz Suarez Marco Sorgato Elisa Cimetta Micro-channels array device fabricated via two photon lithography for cell migration studies in Neuroblastoma metastatic disseminationAll original data are available at: . OpenNano Cancer cell motility Metastasis Microscale devices Two-photon polymerization (2PP) |
| title | Micro-channels array device fabricated via two photon lithography for cell migration studies in Neuroblastoma metastatic disseminationAll original data are available at: . |
| title_full | Micro-channels array device fabricated via two photon lithography for cell migration studies in Neuroblastoma metastatic disseminationAll original data are available at: . |
| title_fullStr | Micro-channels array device fabricated via two photon lithography for cell migration studies in Neuroblastoma metastatic disseminationAll original data are available at: . |
| title_full_unstemmed | Micro-channels array device fabricated via two photon lithography for cell migration studies in Neuroblastoma metastatic disseminationAll original data are available at: . |
| title_short | Micro-channels array device fabricated via two photon lithography for cell migration studies in Neuroblastoma metastatic disseminationAll original data are available at: . |
| title_sort | micro channels array device fabricated via two photon lithography for cell migration studies in neuroblastoma metastatic disseminationall original data are available at |
| topic | Cancer cell motility Metastasis Microscale devices Two-photon polymerization (2PP) |
| url | http://www.sciencedirect.com/science/article/pii/S2352952025000027 |
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