LEA Gene Expression Assessment in Advanced Mutant Rice Genotypes under Drought Stress
Late embryogenesis abundant (LEA) proteins are primarily found in plants stem, roots, and other organs and play significant roles in tolerance to several abiotic stresses. Plants synthesize a discrete set of LEA proteins in response to drought stress. In this study, the expression patterns of LEA ge...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2019/8406036 |
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| author | Zarifth Shafika Kamarudin Mohd Rafii Yusop Mohd Razi Ismail Mahmud Tengku Muda Mohamed Abdul Rahim Harun Oladosu Yusuff Usman Magaji Arolu Fatai |
| author_facet | Zarifth Shafika Kamarudin Mohd Rafii Yusop Mohd Razi Ismail Mahmud Tengku Muda Mohamed Abdul Rahim Harun Oladosu Yusuff Usman Magaji Arolu Fatai |
| author_sort | Zarifth Shafika Kamarudin |
| collection | DOAJ |
| description | Late embryogenesis abundant (LEA) proteins are primarily found in plants stem, roots, and other organs and play significant roles in tolerance to several abiotic stresses. Plants synthesize a discrete set of LEA proteins in response to drought stress. In this study, the expression patterns of LEA genes were investigated in two advanced mutant rice genotypes subjected to the drought stress condition and different physiological traits including photosynthetic rate, leaf chlorophyll content, and photosystem II (PSII) photochemical efficiency (Fv/Fm) which were analyzed to confirm their drought tolerance. Five LEA genes (OsLEA1, OsLEA2, OsLEA3, OsLEA4, and OsLEA5) were used in the evaluation of rice genotypes and were significantly upregulated by more than 4-fold for MR219-4 and MR219-9. The upregulated genes by these two varieties showed high similarity with the drought-tolerant check variety, Aeron1. This indicates that these advanced mutant genotypes have better tolerance to drought stress. The changes in the expression level of LEA genes among the selected rice genotypes under drought stress were further confirmed. Hence, LEA genes could be served as a potential tool for drought tolerance determination in rice. MR219-4 and MR219-9 were found to be promising in breeding for drought tolerance as they offer better physiological adaptation to drought stress. |
| format | Article |
| id | doaj-art-f15e3c990b6e4af5ba2588712695e040 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-f15e3c990b6e4af5ba2588712695e0402025-02-03T06:08:31ZengWileyInternational Journal of Genomics2314-436X2314-43782019-01-01201910.1155/2019/84060368406036LEA Gene Expression Assessment in Advanced Mutant Rice Genotypes under Drought StressZarifth Shafika Kamarudin0Mohd Rafii Yusop1Mohd Razi Ismail2Mahmud Tengku Muda Mohamed3Abdul Rahim Harun4Oladosu Yusuff5Usman Magaji6Arolu Fatai7Department of Crop Sciences, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaDepartment of Crop Sciences, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaInstitute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaDepartment of Crop Sciences, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaAgrotechnology and Biosciences Division, Malaysian Nuclear Agency, 43600 Kajang, Selangor, MalaysiaInstitute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaInstitute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaInstitute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, MalaysiaLate embryogenesis abundant (LEA) proteins are primarily found in plants stem, roots, and other organs and play significant roles in tolerance to several abiotic stresses. Plants synthesize a discrete set of LEA proteins in response to drought stress. In this study, the expression patterns of LEA genes were investigated in two advanced mutant rice genotypes subjected to the drought stress condition and different physiological traits including photosynthetic rate, leaf chlorophyll content, and photosystem II (PSII) photochemical efficiency (Fv/Fm) which were analyzed to confirm their drought tolerance. Five LEA genes (OsLEA1, OsLEA2, OsLEA3, OsLEA4, and OsLEA5) were used in the evaluation of rice genotypes and were significantly upregulated by more than 4-fold for MR219-4 and MR219-9. The upregulated genes by these two varieties showed high similarity with the drought-tolerant check variety, Aeron1. This indicates that these advanced mutant genotypes have better tolerance to drought stress. The changes in the expression level of LEA genes among the selected rice genotypes under drought stress were further confirmed. Hence, LEA genes could be served as a potential tool for drought tolerance determination in rice. MR219-4 and MR219-9 were found to be promising in breeding for drought tolerance as they offer better physiological adaptation to drought stress.http://dx.doi.org/10.1155/2019/8406036 |
| spellingShingle | Zarifth Shafika Kamarudin Mohd Rafii Yusop Mohd Razi Ismail Mahmud Tengku Muda Mohamed Abdul Rahim Harun Oladosu Yusuff Usman Magaji Arolu Fatai LEA Gene Expression Assessment in Advanced Mutant Rice Genotypes under Drought Stress International Journal of Genomics |
| title | LEA Gene Expression Assessment in Advanced Mutant Rice Genotypes under Drought Stress |
| title_full | LEA Gene Expression Assessment in Advanced Mutant Rice Genotypes under Drought Stress |
| title_fullStr | LEA Gene Expression Assessment in Advanced Mutant Rice Genotypes under Drought Stress |
| title_full_unstemmed | LEA Gene Expression Assessment in Advanced Mutant Rice Genotypes under Drought Stress |
| title_short | LEA Gene Expression Assessment in Advanced Mutant Rice Genotypes under Drought Stress |
| title_sort | lea gene expression assessment in advanced mutant rice genotypes under drought stress |
| url | http://dx.doi.org/10.1155/2019/8406036 |
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