Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy
Electron spin resonance (ESR) method is a simple method for detecting various free radicals simultaneously and directly. However, ESR spin trap method is unsuited to analyze weak ESR signals in organs because of water-induced dielectric loss (WIDL). To minimize WIDL occurring in biotissues and to im...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2011/973172 |
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| _version_ | 1841524833868840960 |
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| author | Shinobu Ito Tomohisa Mori Hideko Kanazawa Toshiko Sawaguchi |
| author_facet | Shinobu Ito Tomohisa Mori Hideko Kanazawa Toshiko Sawaguchi |
| author_sort | Shinobu Ito |
| collection | DOAJ |
| description | Electron spin resonance (ESR) method is a simple method for detecting various free radicals simultaneously and directly. However, ESR spin trap method is unsuited to analyze weak ESR signals in organs because of water-induced dielectric loss (WIDL). To minimize WIDL occurring in biotissues and to improve detection sensitivity to free radicals in tissues, ESR cuvette was modified and used with 5,5-dimethtyl-1-pyrroline N-oxide (DMPO). The tissue samples were mouse brain, hart, lung, liver, kidney, pancreas, muscle, skin, and whole blood, where various ESR spin adduct signals including DMPO-ascorbyl radical (AsA∗), DMPO-superoxide anion radical (OOH), and DMPO-hydrogen radical (H) signal were detected. Postmortem changes in DMPO-AsA∗ and DMPO-OOH were observed in various tissues of mouse. The signal peak of spin adduct was monitored until the 205th day postmortem. DMPO-AsA∗ in liver (y=113.8–40.7 log (day), R1=-0.779, R2=0.6, P<.001) was found to linearly decrease with the logarithm of postmortem duration days. Therefore, DMPO-AsA∗ signal may be suitable for detecting an oxidation stress tracer from tissue in comparison with other spin adduct signal on ESR spin trap method. |
| format | Article |
| id | doaj-art-f145135a60b247278f7163dfd28f7150 |
| institution | Kabale University |
| issn | 1687-8191 1687-8205 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Toxicology |
| spelling | doaj-art-f145135a60b247278f7163dfd28f71502025-02-03T05:47:17ZengWileyJournal of Toxicology1687-81911687-82052011-01-01201110.1155/2011/973172973172Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance SpectroscopyShinobu Ito0Tomohisa Mori1Hideko Kanazawa2Toshiko Sawaguchi3I.T.O. Provitamin Research Center, 1-6-7-3F Nakamachi, Musashino, Tokyo, JapanDepartment of Pharmacology and Experimental Neuroscience, College of Medicine, University of Nebraska at Omaha, Omaha, NE 68182, USAFaculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo 105-8512, JapanDepartment of Occupational Therapy, Faculty of Regional Health Therapy, Teikyo Heisei University, 4-1 Uruido-minami, Ichihara, Chiba, JapanElectron spin resonance (ESR) method is a simple method for detecting various free radicals simultaneously and directly. However, ESR spin trap method is unsuited to analyze weak ESR signals in organs because of water-induced dielectric loss (WIDL). To minimize WIDL occurring in biotissues and to improve detection sensitivity to free radicals in tissues, ESR cuvette was modified and used with 5,5-dimethtyl-1-pyrroline N-oxide (DMPO). The tissue samples were mouse brain, hart, lung, liver, kidney, pancreas, muscle, skin, and whole blood, where various ESR spin adduct signals including DMPO-ascorbyl radical (AsA∗), DMPO-superoxide anion radical (OOH), and DMPO-hydrogen radical (H) signal were detected. Postmortem changes in DMPO-AsA∗ and DMPO-OOH were observed in various tissues of mouse. The signal peak of spin adduct was monitored until the 205th day postmortem. DMPO-AsA∗ in liver (y=113.8–40.7 log (day), R1=-0.779, R2=0.6, P<.001) was found to linearly decrease with the logarithm of postmortem duration days. Therefore, DMPO-AsA∗ signal may be suitable for detecting an oxidation stress tracer from tissue in comparison with other spin adduct signal on ESR spin trap method.http://dx.doi.org/10.1155/2011/973172 |
| spellingShingle | Shinobu Ito Tomohisa Mori Hideko Kanazawa Toshiko Sawaguchi Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy Journal of Toxicology |
| title | Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy |
| title_full | Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy |
| title_fullStr | Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy |
| title_full_unstemmed | Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy |
| title_short | Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy |
| title_sort | estimation of the postmortem duration of mouse tissue by electron spin resonance spectroscopy |
| url | http://dx.doi.org/10.1155/2011/973172 |
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