Discovering the interactome, functions, and clinical relevance of enhancer RNAs in kidney renal clear cell carcinoma
Abstract Enhancer RNA (eRNA) has emerged as a key player in cancer biology, influencing various aspects of tumor development and progression. In this study, we investigated the role of eRNAs in kidney renal clear cell carcinoma (KIRC), the most common subtype of renal cell carcinoma. Leveraging high...
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| Format: | Article |
| Language: | English |
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BMC
2025-01-01
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| Series: | BMC Medical Genomics |
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| Online Access: | https://doi.org/10.1186/s12920-024-02081-5 |
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| author | Zhaohui Sun Haojie Du Xudong Zheng Hepeng Zhang Huajie Hu |
| author_facet | Zhaohui Sun Haojie Du Xudong Zheng Hepeng Zhang Huajie Hu |
| author_sort | Zhaohui Sun |
| collection | DOAJ |
| description | Abstract Enhancer RNA (eRNA) has emerged as a key player in cancer biology, influencing various aspects of tumor development and progression. In this study, we investigated the role of eRNAs in kidney renal clear cell carcinoma (KIRC), the most common subtype of renal cell carcinoma. Leveraging high-throughput sequencing data and bioinformatics analysis, we identified differentially expressed eRNAs in KIRC and constructed eRNA-centric regulatory networks. Our findings revealed that up-regulated eRNAs in KIRC potentially regulate immune response and hypoxia pathways, while down-regulated eRNAs may impact ion transport, cell cycle, and metabolism. Furthermore, we developed a diagnostic prediction model based on eRNA expression profiles, demonstrating its effectiveness in KIRC diagnosis. Finally, we elucidated the regulatory mechanism of an eRNA (ENSR00000305834) on the expression of SLC15A2, a potential prognostic biomarker in KIRC, through bioinformatics analysis and in vitro validation experiments. In summary, Our study highlights the clinical significance of eRNAs in KIRC and underscores their potential as therapeutic targets. |
| format | Article |
| id | doaj-art-f1321efc3ae44ea680b81f276212e892 |
| institution | Kabale University |
| issn | 1755-8794 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Medical Genomics |
| spelling | doaj-art-f1321efc3ae44ea680b81f276212e8922025-01-05T12:49:24ZengBMCBMC Medical Genomics1755-87942025-01-0118111410.1186/s12920-024-02081-5Discovering the interactome, functions, and clinical relevance of enhancer RNAs in kidney renal clear cell carcinomaZhaohui Sun0Haojie Du1Xudong Zheng2Hepeng Zhang3Huajie Hu4Yuyao People’s Hospital of Zhejiang ProvinceYuyao People’s Hospital of Zhejiang ProvinceYuyao People’s Hospital of Zhejiang ProvinceYuyao People’s Hospital of Zhejiang ProvinceYuyao People’s Hospital of Zhejiang ProvinceAbstract Enhancer RNA (eRNA) has emerged as a key player in cancer biology, influencing various aspects of tumor development and progression. In this study, we investigated the role of eRNAs in kidney renal clear cell carcinoma (KIRC), the most common subtype of renal cell carcinoma. Leveraging high-throughput sequencing data and bioinformatics analysis, we identified differentially expressed eRNAs in KIRC and constructed eRNA-centric regulatory networks. Our findings revealed that up-regulated eRNAs in KIRC potentially regulate immune response and hypoxia pathways, while down-regulated eRNAs may impact ion transport, cell cycle, and metabolism. Furthermore, we developed a diagnostic prediction model based on eRNA expression profiles, demonstrating its effectiveness in KIRC diagnosis. Finally, we elucidated the regulatory mechanism of an eRNA (ENSR00000305834) on the expression of SLC15A2, a potential prognostic biomarker in KIRC, through bioinformatics analysis and in vitro validation experiments. In summary, Our study highlights the clinical significance of eRNAs in KIRC and underscores their potential as therapeutic targets.https://doi.org/10.1186/s12920-024-02081-5eRNAKIRCE-P loopsTFSLC15A2ENSR00000305834 |
| spellingShingle | Zhaohui Sun Haojie Du Xudong Zheng Hepeng Zhang Huajie Hu Discovering the interactome, functions, and clinical relevance of enhancer RNAs in kidney renal clear cell carcinoma BMC Medical Genomics eRNA KIRC E-P loops TF SLC15A2 ENSR00000305834 |
| title | Discovering the interactome, functions, and clinical relevance of enhancer RNAs in kidney renal clear cell carcinoma |
| title_full | Discovering the interactome, functions, and clinical relevance of enhancer RNAs in kidney renal clear cell carcinoma |
| title_fullStr | Discovering the interactome, functions, and clinical relevance of enhancer RNAs in kidney renal clear cell carcinoma |
| title_full_unstemmed | Discovering the interactome, functions, and clinical relevance of enhancer RNAs in kidney renal clear cell carcinoma |
| title_short | Discovering the interactome, functions, and clinical relevance of enhancer RNAs in kidney renal clear cell carcinoma |
| title_sort | discovering the interactome functions and clinical relevance of enhancer rnas in kidney renal clear cell carcinoma |
| topic | eRNA KIRC E-P loops TF SLC15A2 ENSR00000305834 |
| url | https://doi.org/10.1186/s12920-024-02081-5 |
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