Optimization of UAE-NADES green extraction of bioactive compounds from chickpea (Cicer arietinum L.) sprouts using simplex lattice mixture design methodology
In the present study, a statistical tool called the simplex lattice mixture design method was used to create a new formulation of Natural Deep Eutectic Solvent (NADES), which is derived from a combination of three compounds (citric acid, glycerol, and water) to extract bioactive compounds from chick...
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Elsevier
2025-01-01
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| Series: | Ultrasonics Sonochemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417724004358 |
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| author | Waseem Khalid Hyrije Koraqi Imed E Benmebarek Andrés Moreno Tawfiq Alsulami Robert Mugabi Gulzar Ahmad Nayik |
| author_facet | Waseem Khalid Hyrije Koraqi Imed E Benmebarek Andrés Moreno Tawfiq Alsulami Robert Mugabi Gulzar Ahmad Nayik |
| author_sort | Waseem Khalid |
| collection | DOAJ |
| description | In the present study, a statistical tool called the simplex lattice mixture design method was used to create a new formulation of Natural Deep Eutectic Solvent (NADES), which is derived from a combination of three compounds (citric acid, glycerol, and water) to extract bioactive compounds from chickpea (Cicer arietinum L.) sprouts. The mixture (natural deep eutectic solvent) was formulated by combining three solvents including citric acid, glycerol, and water. The extraction was performed in a sonication bath for 30 min. The simultaneous optimization was performed to obtain the highest total polyphenol content (TPC), total flavonoid content (TFC) and antioxidants activity. The highest values of total polyphenol content (TPC), total flavonoid content (TFC) and antioxidant activity were 128.0 ± 0.2 mg GAE/100 g, 38.61 ± 0.03 mg CE/100 g and 2117 ± 1.8 µmol TE/100 g respectively. HPLC-DAD of the optimized extract was utilized for quantification of polyphenol compounds showing catechin as the main compound followed by chlorogenic acid, epicatechin, syringic acid, rutin, gallic acid, kaempferol 3-glucoside, ferulic acid, and coumaric acid. These findings may represent a significant advancement in the management of phenolic compound extraction for targeted uses, such as serving as alternatives to traditional antioxidants primarily employed in the food industry to improve nutritional quality. Furthermore, our research has shown that mixture designs are an efficient and useful method for structuring and optimizing experimental parameters to achieve the most accurate results with the minimum number of experiments. |
| format | Article |
| id | doaj-art-f11120ac86d640979aee081ff813b4b7 |
| institution | Kabale University |
| issn | 1350-4177 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj-art-f11120ac86d640979aee081ff813b4b72025-01-11T06:38:46ZengElsevierUltrasonics Sonochemistry1350-41772025-01-01112107186Optimization of UAE-NADES green extraction of bioactive compounds from chickpea (Cicer arietinum L.) sprouts using simplex lattice mixture design methodologyWaseem Khalid0Hyrije Koraqi1Imed E Benmebarek2Andrés Moreno3Tawfiq Alsulami4Robert Mugabi5Gulzar Ahmad Nayik6Department of Organic Chemistry, Faculty of Chemical Sciences and Technologies, University of Castilla La Mancha, 13071 Ciudad Real, Spain; Department of Molecular Food Chemistry and Food Development, Institute of Food and One Health, Gottfried Wilhelm Leibniz University Hannover, Hannover, GermanyFaculty of Food Science and Biotechnology, UBT-Higher Education Institution, Pristina-KosovoDepartment of Organic Chemistry, Faculty of Chemical Sciences and Technologies, University of Castilla La Mancha, 13071 Ciudad Real, SpainDepartment of Organic Chemistry, Faculty of Chemical Sciences and Technologies, University of Castilla La Mancha, 13071 Ciudad Real, Spain; Corresponding authors.Department of Food Science & Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Food Technology and Nutrition, Makerere University, Kampala, Uganda; Corresponding authors.Marwadi University Research Centre, Department of Microbiology, Marwadi University, Rajkot, Gujarat 360003, India; Corresponding authors.In the present study, a statistical tool called the simplex lattice mixture design method was used to create a new formulation of Natural Deep Eutectic Solvent (NADES), which is derived from a combination of three compounds (citric acid, glycerol, and water) to extract bioactive compounds from chickpea (Cicer arietinum L.) sprouts. The mixture (natural deep eutectic solvent) was formulated by combining three solvents including citric acid, glycerol, and water. The extraction was performed in a sonication bath for 30 min. The simultaneous optimization was performed to obtain the highest total polyphenol content (TPC), total flavonoid content (TFC) and antioxidants activity. The highest values of total polyphenol content (TPC), total flavonoid content (TFC) and antioxidant activity were 128.0 ± 0.2 mg GAE/100 g, 38.61 ± 0.03 mg CE/100 g and 2117 ± 1.8 µmol TE/100 g respectively. HPLC-DAD of the optimized extract was utilized for quantification of polyphenol compounds showing catechin as the main compound followed by chlorogenic acid, epicatechin, syringic acid, rutin, gallic acid, kaempferol 3-glucoside, ferulic acid, and coumaric acid. These findings may represent a significant advancement in the management of phenolic compound extraction for targeted uses, such as serving as alternatives to traditional antioxidants primarily employed in the food industry to improve nutritional quality. Furthermore, our research has shown that mixture designs are an efficient and useful method for structuring and optimizing experimental parameters to achieve the most accurate results with the minimum number of experiments.http://www.sciencedirect.com/science/article/pii/S1350417724004358Chickpea sproutsNovel extractionMixture design-simplex latticeAntioxidants polyphenol content |
| spellingShingle | Waseem Khalid Hyrije Koraqi Imed E Benmebarek Andrés Moreno Tawfiq Alsulami Robert Mugabi Gulzar Ahmad Nayik Optimization of UAE-NADES green extraction of bioactive compounds from chickpea (Cicer arietinum L.) sprouts using simplex lattice mixture design methodology Ultrasonics Sonochemistry Chickpea sprouts Novel extraction Mixture design-simplex lattice Antioxidants polyphenol content |
| title | Optimization of UAE-NADES green extraction of bioactive compounds from chickpea (Cicer arietinum L.) sprouts using simplex lattice mixture design methodology |
| title_full | Optimization of UAE-NADES green extraction of bioactive compounds from chickpea (Cicer arietinum L.) sprouts using simplex lattice mixture design methodology |
| title_fullStr | Optimization of UAE-NADES green extraction of bioactive compounds from chickpea (Cicer arietinum L.) sprouts using simplex lattice mixture design methodology |
| title_full_unstemmed | Optimization of UAE-NADES green extraction of bioactive compounds from chickpea (Cicer arietinum L.) sprouts using simplex lattice mixture design methodology |
| title_short | Optimization of UAE-NADES green extraction of bioactive compounds from chickpea (Cicer arietinum L.) sprouts using simplex lattice mixture design methodology |
| title_sort | optimization of uae nades green extraction of bioactive compounds from chickpea cicer arietinum l sprouts using simplex lattice mixture design methodology |
| topic | Chickpea sprouts Novel extraction Mixture design-simplex lattice Antioxidants polyphenol content |
| url | http://www.sciencedirect.com/science/article/pii/S1350417724004358 |
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