Integrated multi-omics reveals different host crosstalk of atopic dermatitis-enriched Bifidobacterium longum Strains

Abstract The infant gut microbiome is essential for long-term health and is linked to atopic dermatitis (AD), although the underlying mechanisms are not fully understood. This study investigated gut microbiome-host interactions in 31 infants with AD and 29 healthy controls using multi-omics approach...

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Main Authors: Hoon Je Seong, Yoon Mee Park, Bong-Soo Kim, Hyun Ju Yoo, Taeyune Kim, Sun Mi Yoon, Jeong-Hyun Kim, So-Yeon Lee, Yun Kyung Lee, Dong-Woo Lee, Myung Hee Nam, Soo-Jong Hong
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:npj Biofilms and Microbiomes
Online Access:https://doi.org/10.1038/s41522-025-00714-w
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Summary:Abstract The infant gut microbiome is essential for long-term health and is linked to atopic dermatitis (AD), although the underlying mechanisms are not fully understood. This study investigated gut microbiome-host interactions in 31 infants with AD and 29 healthy controls using multi-omics approaches, including metagenomic, host transcriptomic, and metabolomic analyses. Microbial diversity was significantly altered in AD, with Bifidobacterium longum and Clostridium innocuum associated with these changes. At the strain-level, only B. longum differed significantly between groups, with pangenome analyses identifying genetic variations potentially affecting amino acid and lipid metabolites. Notably, B. longum subclade I, which was more prevalent in healthy controls, correlated with host transcriptomic pathways involved in phosphatidylinositol 3-kinase-AKT signaling and neuroactive ligand-receptor pathways, as well as specific metabolites, including tetrahydrocortisol and ornithine. These findings highlight the role of B. longum strain-level variation in infants, offering new insights into microbiome-host interactions related to AD.
ISSN:2055-5008